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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔡偉博(Wei-bor Tsai) | |
| dc.contributor.author | Wei-Ting Lin | en |
| dc.contributor.author | 林韋廷 | zh_TW |
| dc.date.accessioned | 2021-06-16T05:30:20Z | - |
| dc.date.available | 2019-08-22 | |
| dc.date.copyright | 2014-08-22 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-13 | |
| dc.identifier.citation | [1] Glazer ES, Curley SA. The Ongoing History of Thermal Therapy for Cancer.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56473 | - |
| dc.description.abstract | 溫熱療法，將癌細胞組織加熱到 41~45 oC 可以明顯增加使用放射或是化學
性治療法的效果。本論文目的是為了發展出含有光敏感分子之電紡絲應用於溫熱 至療法來治療表面癌症腫瘤，例如皮膚癌。聚左乳酸與銅葉綠素鈉所噴成的電紡 絲，可以經由照射 532 nm 的綠光雷射來加熱，達到殺死細胞的目的。 在第二部分我們將苯乙烯與銅葉綠素鈉聚合以後，得到接枝有銅葉綠素鈉單 體的聚苯乙烯，並且將此產物噴成電紡絲後來應用，之後經由 MTT assay、染 trypan blue、live/dead 三種實驗來觀察綠光雷射強度與照射時間對細胞的影響，最後達到可以控制照射綠光雷射的強度與照射時間，來達到殺死癌細胞的目的，並且不會對周遭正常細胞造成明顯損害，我們可以將此結果應用於溫熱治療法來治療大範圍表面癌症腫瘤。 | zh_TW |
| dc.description.abstract | Hyperthermia, the heating of cancerous tissues to between 41 and 45 oC, has
been shown to improve the efficacy of cancer therapy when used in conjunction with irradiation and/or chemotherapy. The objective of this study was to develop an incorporation of electrospun sheet with photosensitizer for photodynamic therapy in surface cancer tumor, such as skin cancer. The blend of poly-L-lactic acid (PLLA) and chlorophyllin sodium copper (CSC) was electrospinning a sheet was developed. The electrospun sheets could be remotely heated upon exposure to an external wave length 532 nm green laser to kill cells. In the second part of the study, for the purpose to produce high percentage chlorophyllin sodium copper contain polystyrene , we used chlorophyllin sodium copper and styrene with ATRP, and electrospinning to sheet for the hyperthermia purpose. Then we used MTT assay、trypan blue stain and live/dead stain to observe the effect of green laser intensity and irradiation time to the cell viability. Finally we can achieve the purpose of killing cancer cell by control the green laser intensity and irradiation time without hurting the normal cell around. We can use this result on the hyperthermia of in surface cancer tumor, such as skin cancer. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T05:30:20Z (GMT). No. of bitstreams: 1 ntu-103-R01524081-1.pdf: 2326908 bytes, checksum: 5369865cda03a6318ccb50fd3707c522 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | Content
Content ...................................................................................................................................... iv List of Tables .......................................................................................................................... viii List of Figures .......................................................................................................................... ix Chapter 1 Introduction ............................................................................................................. 1 1.1 Thermal therapy for cancer ........................................................................................... 1 1.1.1 Hyperthermia ........................................................................................................... 2 1.1.2 Photothermal therapy .............................................................................................. 3 1.2 Photosensitizer ................................................................................................................ 4 1.2.1 Overview of photosensitizer ..................................................................................... 4 1.2.2 Porphyrin-based molecules ..................................................................................... 7 1.3 Electrospinning ............................................................................................................. 11 1.3.1 Overview of electrospinning ............................................................................. 11 1.3.2 Electrospinning parameters ............................................................................. 14 1.4 Motive and aim ............................................................................................................. 15 1.5 Research frame work .................................................................................................... 16 Chapter 2 Materials and Methods .......................................................................................... 17v 2.1 Materials ................................................................................................................ 17 2.1.1 Photosensitizer ....................................................................................................... 17 2.1.2 Electrospinning ...................................................................................................... 17 2.1.3 Cytotoxicity assay (3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazoliumbromide, MTT assay) ..................................................................................................................... 17 2.1.4 Mouse fibroblast-like cell line L929 culture ......................................................... 17 2.2 Experimental instrument and materials ...................................................................... 18 2.2.1 Experimental instrument .................................................................................. 18 2.2.2 Experimental consumables............................................................................. 19 2.3 Solution formula........................................................................................................... 20 2.4 Methods ......................................................................................................................... 21 2.4.1 Porphyrin-based molecules heat efficiency comparison ...................................... 21 2.4.2 Evaluation of the cytotoxicity of CSC ................................................................... 21 2.4.3 Thermal properties of CSC .................................................................................... 22 2.4.4 Preparation of photosensitizer contained polymer solution. ................................ 22 2.4.5 Synthesis of styrene and photosensitive copolymer .............................................. 23 2.4.6 Preparation of electrospinning nanofiber sheets ................................................. 23vi 2.4.7 Structure of PLLA and PLLA/CSC fibers ............................................................ 24 2.4.8 Cytotoxic assay of PLLA/CSC electrospinning sheets ......................................... 24 2.4.9 Photothermal Conversion Experiments ................................................................ 25 2.4.10 Photothermal hyperthermia on L929 Cells ........................................................ 25 2.4.11 Photothermal hyperthermia via culturing cells on TCPS cover by PLLA/CSC sheets ............................................................................................................................... 26 Chapter 3 Characterization and photosensity analysis of PLLA/CSC electrospinning sheets ................................................................................................................................................. 27 3.1 Porphyrin-based molecules heat efficiency comparison ............................................. 27 3.2 Synthesis of PLLA/CSC electrospun ........................................................................... 27 3.3 Structure of PLLA and PLLA/CSC fibers ................................................................... 27 3.4 Cytotoxic assay of CSC ................................................................................................. 28 3.5 Cytotoxic assay of PLLA/CSC electrospinning sheets ................................................ 28 3.6 Thermal properties of CSC ........................................................................................... 29 3.7 Thermal properties of PLLA/CSC eletrospinning nanofiber sheet ............................ 29 3.8 L929 adhesion and proliferation on PLLA/CSC fibers ............................................... 30 3.9 Irradiation effects to L929 cells ................................................................................... 30vii 3.10 Photothermal hyperthermia via culturing cells on PLLA/CSC sheets ..................... 30 3.11 Photothermal hyperthermia via culturing cells on TCPS cover by PLLA/CSC sheets ............................................................................................................................................. 31 3.12 Discussion ................................................................................................................... 32 Chapter4 Characterization and photosensity analysis of PS-CSC electrospinning sheets .. 49 4.1 Synthesis of PS-CSC ..................................................................................................... 49 4.2 Structure of PS and PS-CSC fibers ............................................................................. 49 4.3 Thermal properties of PS-CSC eletrospinning nanofiber sheet ................................. 50 4.4 Photothermal hyperthermia via culturing cells on TCPS cover by PS-CSC sheets ... 50 4.5 Discussion ..................................................................................................................... 51 Chapter 5 Conclusion ............................................................................................................. 57 Reference ................................................................................................................................ 58 | |
| dc.language.iso | en | |
| dc.subject | 綠光雷射 | zh_TW |
| dc.subject | 光熱療法 | zh_TW |
| dc.subject | 電訪絲 | zh_TW |
| dc.subject | electropsun | en |
| dc.subject | photothermal therapies | en |
| dc.subject | green laser | en |
| dc.title | 含光敏感分子之奈米電紡絲纖維應用於癌症之熱治療 | zh_TW |
| dc.title | Electrospun Nanofibers Containing photosensitive polymer for hyperthermia in Cancer Therapy | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 游佳欣(Jiashing Yu),林睿哲(Jui-Che Lin) | |
| dc.subject.keyword | 電訪絲,光熱療法,綠光雷射, | zh_TW |
| dc.subject.keyword | electropsun,photothermal therapies,green laser, | en |
| dc.relation.page | 60 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-14 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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