Andrographolide對於傷口癒合之促進效用：活體與細胞上的研究

Abstract

Wound healing is a strictly regulated process. Once the process is not well controlled, patients suffered from the pain and it may cause serious outcome, such as amputation. Finding drugs to accelerate wound healing process is necessary and urgent. Andrographolide (AND) is a major bioactive phytoconstituent in various parts of A. paniculata, particularly in the leaves. AND contains anti-inflammatory, antibacterial, and antifungal activities. Whether AND can accelerate wound healing is still not understood, this study explore the effects of AND on skin wound healing process and its underlying mechanisms. C57BL/6 mice were used in the in vivo wound healing assay. Mice were anesthetized and two wounds were made on the back. Hydrogen gel containing DMSO (CTL) or AND was administered on the wounds every day. Pictures were taken every two days, and the surface wound areas were quantified by Image Pro. Wounds were then cut on day 3、5 and 7 to analyze the histological changes of skin with H&E and trichrome stain. The wound size was smaller and the collagen synthesis was more in AND treated wound. With these analysis, AND can significantly accelerate wound healing. Next, we further investigated the mechanisms underlying AND-accelerated wound healing. With in vitro cell model, macrophages and fibroblasts are key modulators of wound healing process in the inflammation and proliferation phases. Raw 264.7 and NIH3T3 were used to observe the AND-induced anti-inflammatory, cell proliferative, migratory, and collagen productive effects. AND can not only significantly attenuate LPS-induced COX-2 expression and NO production in macrophages, but also expedite the proliferation and migration in fibroblasts through ERK and PI3K/AKT signaling pathways. With the results in the in vivo and in vitro models, AND has the potential to be a new drug for wound healing. Further development of AND may lead to a beneficial therapeutic agent for the treatment of wound healing disease and shorten the healing time of wound.