中波段紅外線的抗乳癌效果研究

Abstract

Broad-band infrared (IR) light is used in scientific, military, medical and industrial applications, but only a few about narrow band IR radiation is applied in biomedical uses. Breast cancer is one of the cancer-related leading causes of death worldwide. Breast cancer-related mortality is associated with the development of metastatic potential to lymph nodes and distant organs. Therefore, we aimed to investigate the effects of narrow band MIR on metastatic breast cancer cells. Middle infrared (MIR) in the wavelength range of 3 to 5 μm region was used to irradiate MDA-MB-231 breast cancer cells. In our results, we found MIR exposure inhibited breast cancer cell proliferation and induced morphological changes but did not affect normal breast cells. To figure out the MIR-triggered molecular mechanism in breast cancer cells, we performed isobaric tags for relative and absolute quantification (iTRAQ)-coupled LC-MS/MS system and identified 81 up-regulated and 86 down-regulated proteins. We identified these differentially expressed proteins were contributing to cell cycle progression and focal adhesion by bioinformatics functional enrichment analysis. Furthermore, we used western blotting to validate the differentially expressed proteins and the results showed the consistent with iTRAQ data. We also found that MIR increased the accumulation of G2/M cell population during cell cycle progression and the microtubule arrangement of MIR-exposed cells showed formed microtubule-organizing center (MTOC) without condensed chromatin, which is the feature of late interphase. These evidences suggested that MIR reduced cell proliferation by inhibiting mitosis process and migration activity and invasion ability by altering the localization of the focal adhesion molecules. In summary, MIR could inhibit MDA-MB-231 cell proliferation, migration activity and invasion ability by changing the protein expression level. Our finding suggest that MIR could be a potential implementation of breast cancer therapy.