光質對水耕栽培薏苡芽菜抗氧化活性之影響

Abstract

芽菜，無汙染且高營養價值是目前受青睞的綠色食品，無論是豆類、穀類或蔬菜類等，皆可以芽菜栽培模式進行生產。本研究將探討光質對水耕栽培薏苡芽菜之抗氧化活性影響，糙薏仁先經消毒、浸種及催芽步驟後，移植至不同光線和水耕液的生長條件下進行發芽，並於栽培過程中以總酚含量、清除DPPH自由基能力及總抗氧化能力 (Trolox equivalent antioxidant capacity) 指標，來評估薏苡芽菜機能性功效。 結果顯示，光線和水耕液會產生交互作用。在去離子水栽培條件下藍光和紅光會抑制芽菜莖的伸長，而養液栽培則反之。經由光線的照射可提高芽菜總多酚含量，在栽培第三天時，白光組、藍光組及紅光組其總多酚含量皆較黑暗組高，分別為提高了41%、68%及18%；在栽培第七天，僅有藍光組總酚類含量較黑暗組高出40%；而養液栽培其總多酚含量低於去離子水栽培。皮爾遜相關性分析可知總酚類含量與清除DPPH自由基能力及總抗氧化能力之間的相關係數為0.70、0.69，而清除DPPH自由基能力和總抗氧化能力的相關係為為0.58。表示總多酚含量與抗氧化活性有密切的相關。 薏苡種子透過發芽可增加本身的營養價值，亦能以芽菜進行生產，薏苡芽菜富含豐富的酚類化合物，賦有抗氧化機能性，極具保健素材之潛力。

Sprouts are currently favored in the world, either beans, grains or vegetables can used to be sprout. This study established adlay sprout cultivation model. Adlay seed via disinfection, soaking and priming ,then transfered to germination under different lighting sources and hydroponic solutions . Using the phenolic contents (TPC), scavenging DPPH radicals ability (DPPH) and Trolox equivalent antioxidant capacity (TEAC) to assess the functional of adlay sprout. The results show that interaction between lights and hydroponic solutions. Blue and red light are slightly inhibit the growth of sprout with water hydroponic solution. On the contrary, blue and red light promote the growth of sprout with nutrient solution. But light illumination can enhance the total polyphenol content of adlay sprout. On 3 days after sowing, the total polyphenol content of the white, blue and red groups with water hydroponic solution were higher than the darkness control by 41%, 68 % and 18% respectively. On 7 DAS, only the blue group was 40% higher than the darkness control in the total phenol content. Using the Pearson correlation analysis the coefficient among these antioxidant capacities. The coefficient between TPC and DPPH is 0.69. TPC and TEAC is 0.69. DPPH and TEAC is 0.58. Data shows the total polyphenol content and antioxidant capacities are closely related. Adlay seed increase the nutritional value by germination, and it can be used to produce sprouts. Adlay sprouts are rich in phenolic compounds and excellent antioxidant capacity. It has the potential in the nutraceutical applications.