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標題: | 利用介電泳與微流技術捕捉與分離細胞 Cell Capture and Separation Using Dielectrophoresis and Microfluidics |
作者: | Ya-Chu Chang 張雅筑 |
指導教授: | 李雨 |
關鍵字: | 生物晶片,介電泳力,細胞捕捉與分離, Microfluidics device,Dielectrophoresis,Particle capture and separation, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 本論文工作乃利用微流及介電泳技術,設計並製作一於流動液體中捕捉與分離粒子(含細胞)的微流裝置。該裝置在一微流道頂壁建有凹槽,在凹槽上下游流道底壁建有電極,以産生負介電泳力選擇性地將具特定Kr值(柯莫氏因子的實部)的粒子推入凹槽内、具其他Kr值的粒子則被流動液體帶往下游,而得以將不同介電特性的粒子分離。此裝置為他人在文獻中所提出者,本文將該裝置修改使能對欲分離的兩種粒子分别精確計數、而能算出對目標粒子在給定參數下的捕捉率及分離純度,所以本文所發展裝置能用於探取分離不同粒子所需的適當操作參數、而是此類以粒子固有物理特性作分離依據的裝置所必需的。就聚苯乙烯(PS)粒子與癌細胞(含肺癌細胞CL1-0與CL1-5及腸癌細胞Colo205)的分離,在操作頻率1MHz、流量40ul/hr以下時,二者可100%分離;就CL1-0與Colo205在導電度0.11S/m的溶液中,在流量30ul/hr及操作頻率為70kHz時分離率可達80%以上。本文亦對所提出裝置在幾何及操作參數上作了數值及實驗分析。 A device for selective particle capture and separation using microfluidics and dielectrophoresis was developed in the thesis. The device is a micro channel with grooves built on its ceiling. Electrodes are built on the bottom surface of the device on both the upstream and downstream sides of the groove. Negative dielectrophoretic force is exerted on the test particle when it passes through the electrode gap. Particles with selected Kr (the real part of the Clausius-Mossotti factor) will be pushed into the channel and are thus captured. Other particles with different Kr will be carried downstream, and thus particles with different dielectric properties are separated. The device was reported in the literature, but here we propose a modify version of the device such that the two particles to be separated can be counted, and thus the capture rate and purity of separation can be evaluated accurately. Therefore, the modified device proposed here is capable of determining suitable operation parameters for a given separation, which is necessary for a separating device based on the intrinsic physical properties of particles. As for the separation of polystyrene particles and cancer cells (including the lung cancer cells CL1-0 and CL1-5, and the colorectal cancer cells Colo205), 100% can be separated under a frequency 1 MHz and a background flow rate below 40ul/hr. 80% separation can be achieved for the mixture of CL1-0 and Colo205 in a medium with conductivity 0.11S/m, at a flow rate 30ul/hr and frequency 70kHz. The geometric and operation parameters of the device were also studied numerically and experimentally. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54918 |
全文授權: | 有償授權 |
顯示於系所單位: | 應用力學研究所 |
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ntu-103-1.pdf 目前未授權公開取用 | 3.14 MB | Adobe PDF |
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