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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54918Full metadata record
| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 李雨 | |
| dc.contributor.author | Ya-Chu Chang | en |
| dc.contributor.author | 張雅筑 | zh_TW |
| dc.date.accessioned | 2021-06-16T03:41:32Z | - |
| dc.date.available | 2018-03-13 | |
| dc.date.copyright | 2015-03-13 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2015-02-12 | |
| dc.identifier.citation | 參考文獻及書目
[1] 行政院衛生署, “101年國人主要死因統計結果”,2013 / 06 / 06 [2] Wang, X. B., Huang, Y., Holzel, R., Burt, J. P. H. and Pethig, R., “Theoretical and experimental investigations of the interdependence of the dielectric, dielectrophoretic and electrorotational behaviour of colloidal particles”, J. Phys. D: Appl. Phys., 26, 312-322, (1993). [3] Ormerod, M. G., “Flow Cytometry, 3rd Ed,” Oxford University Press, (2000). [4] Lo, Y. J., Lei, U and Yang, P. C., “Selective separation and isolation of particles/cells of similar sizes using dielectrophoresis,” MicroTAS 2011 Conference, Paper No. M21G, 2-6 October 2011, Seattle, USA. [5] Lo Y. J., “Generalized Dielectrophoresis near Walls – Theory, Experiment and Application,” Doctoral Dissertation,National Taiwan University,(2010). [6] 李東霖, “在微流系統中利用介電泳力捕捉及分離微粒與細胞”, 國立台灣大學應用力學所碩士論文, (2013). [7] Chuang, C.-H. and Huang,Y.-W., “Condensation of fluorescent nanoparticles using a DEP chipwith a dot-electrode array”, Microelectronic Engineering 97 317–323,( 2012). [8] Alshareef, M., Metrakos, N., Perez, E. J., Azer, F., Yang, F., Yang, X. and Wang, G., “Separation of tumor cells with dielectrophoresis-based microfluidic chip” Biomicrofluidics 7, 011803,(2013). [9] Wang, M.-W., “Using Dielectrophoresis to Trap Nanobead/Stem CellCompounds in Continuous Flow”, Journal of The Electrochemical Society, 156(8), G97-G102, (2009). [10] Suehiro, J., Ohtsubo, A., Hatano, T. and Hara, M., “Selective detection of bacteria by adielectrophoretic impedance measurement method using an anti-body immobilzed electrodechip”. Sens. Actuators B 119, 319–326, (2006). [11] Iliescu, C., Tresset, G., Yu, L. and Xu, G., “3D Dielectrophoretic Chips: Trappingand Separation of Cell Populations”, Romanian Journal of Information Science and Technology, 13,49-64, (2010). [12] Pohl, H.A.,“The Motion and precipitation of suspensoids in divergent electric fields,” J. Appl. Phys. 22, 869 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54918 | - |
| dc.description.abstract | 本論文工作乃利用微流及介電泳技術，設計並製作一於流動液體中捕捉與分離粒子(含細胞)的微流裝置。該裝置在一微流道頂壁建有凹槽，在凹槽上下游流道底壁建有電極，以産生負介電泳力選擇性地將具特定Kr值(柯莫氏因子的實部)的粒子推入凹槽内、具其他Kr值的粒子則被流動液體帶往下游，而得以將不同介電特性的粒子分離。此裝置為他人在文獻中所提出者，本文將該裝置修改使能對欲分離的兩種粒子分别精確計數、而能算出對目標粒子在給定參數下的捕捉率及分離純度，所以本文所發展裝置能用於探取分離不同粒子所需的適當操作參數、而是此類以粒子固有物理特性作分離依據的裝置所必需的。就聚苯乙烯(PS)粒子與癌細胞(含肺癌細胞CL1-0與CL1-5及腸癌細胞Colo205)的分離，在操作頻率1MHz、流量40ul/hr以下時，二者可100%分離；就CL1-0與Colo205在導電度0.11S/m的溶液中，在流量30ul/hr及操作頻率為70kHz時分離率可達80%以上。本文亦對所提出裝置在幾何及操作參數上作了數值及實驗分析。 | zh_TW |
| dc.description.abstract | A device for selective particle capture and separation using microfluidics and dielectrophoresis was developed in the thesis. The device is a micro channel with grooves built on its ceiling. Electrodes are built on the bottom surface of the device on both the upstream and downstream sides of the groove. Negative dielectrophoretic force is exerted on the test particle when it passes through the electrode gap. Particles with selected Kr (the real part of the Clausius-Mossotti factor) will be pushed into the channel and are thus captured. Other particles with different Kr will be carried downstream, and thus particles with different dielectric properties are separated. The device was reported in the literature, but here we propose a modify version of the device such that the two particles to be separated can be counted, and thus the capture rate and purity of separation can be evaluated accurately. Therefore, the modified device proposed here is capable of determining suitable operation parameters for a given separation, which is necessary for a separating device based on the intrinsic physical properties of particles. As for the separation of polystyrene particles and cancer cells (including the lung cancer cells CL1-0 and CL1-5, and the colorectal cancer cells Colo205), 100% can be separated under a frequency 1 MHz and a background flow rate below 40ul/hr. 80% separation can be achieved for the mixture of CL1-0 and Colo205 in a medium with conductivity 0.11S/m, at a flow rate 30ul/hr and frequency 70kHz. The geometric and operation parameters of the device were also studied numerically and experimentally. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T03:41:32Z (GMT). No. of bitstreams: 1 ntu-103-R01543027-1.pdf: 3215157 bytes, checksum: fff40198d60468e17721f4ad83f177ee (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書 # 誌謝 I 中文摘要 II ABSTRACT III 目錄 IV 圖目錄 VII 表目錄 IX 第1章 緒論 1 1.1 研究背景與動機 1 1.2 研究目的 2 1.3 文獻回顧 6 1.4 本文架構 7 第2章 理論 9 2.1 有效偶極矩 9 2.2 介電泳力介紹 11 2.3 等效圓球模型 13 2.4 正負介電泳力 14 2.5 流體拖曳力 17 2.6 粒子/細胞軌跡 18 第3章 實驗方法與設備 19 3.1 運用微機電技術製作電極晶片 20 3.1.1 電極設計 20 3.1.2 清洗基材 21 3.1.3 金屬層蒸鍍 22 3.1.4 電極微影製程 23 3.2 利用微機電技術製作微流道晶片 26 3.2.1 微流道設計 26 3.2.2 微流道母模製作 26 3.2.3 PDMS澆注與翻模 29 3.2.4 元件接合與外部連結 29 3.3 實驗溶液與生物微粒選配 30 3.3.1 細胞培養液的調配 31 3.3.2 肺腺癌細胞(CL1-0、CL1-5)繼代培養與冷凍保存 31 3.3.3 人體結腸癌細胞(Colo205)繼代培養與冷凍保存 33 3.3.4 實驗溶液的調配 33 3.3.5 實驗細胞螢光處理 34 3.4 實驗設備與架設 35 3.4.1 實驗設備 35 3.4.2 實驗架設 37 3.5 COMSOL計算的邊界設定 38 第4章 實驗結果與討論 40 4.1 實驗測量方式與定義 40 4.1.1 捕捉率、分離率與純度定義 42 4.1.2 細胞計數與尺寸量測 43 4.2 單入口流道-細胞捕捉 45 4.2.1 凹槽高度對捕捉力的影響 48 4.2.2 凹槽寬度對捕捉力的影響 51 4.2.3 電極間距對捕捉力的影響 53 4.2.4 微流道高度對捕捉率的影響 53 4.2.5 PS particle捕捉率實驗 55 4.3 雙入口流道-微粒分離 58 4.3.1 PS particle與細胞分離結果 59 4.3.2 CL1-0（FL）與CL1-5分離結果 62 4.4 Colo205與CL1-0（FL）分離結果 62 第5章 結論與未來展望 65 5.1 結論 65 5.2 未來展望 65 參考文獻及書目 67 | |
| dc.language.iso | zh-TW | |
| dc.subject | 細胞捕捉與分離 | zh_TW |
| dc.subject | 生物晶片 | zh_TW |
| dc.subject | 介電泳力 | zh_TW |
| dc.subject | Microfluidics device | en |
| dc.subject | Particle capture and separation | en |
| dc.subject | Dielectrophoresis | en |
| dc.title | 利用介電泳與微流技術捕捉與分離細胞 | zh_TW |
| dc.title | Cell Capture and Separation
Using Dielectrophoresis and Microfluidics | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 楊政穎,江宏仁,許聿翔 | |
| dc.subject.keyword | 生物晶片,介電泳力,細胞捕捉與分離, | zh_TW |
| dc.subject.keyword | Microfluidics device,Dielectrophoresis,Particle capture and separation, | en |
| dc.relation.page | 69 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-02-12 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| Appears in Collections: | 應用力學研究所 | |
Files in This Item:
| File | Size | Format | |
|---|---|---|---|
| ntu-103-1.pdf Restricted Access | 3.14 MB | Adobe PDF |
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