加壓催產素在斑馬魚離子調節機制中扮演的角色

Abstract

血管加壓素(AVP)是由大腦下視丘神經元製造，儲存在腦下垂體後葉的荷爾蒙，先前許多研究發現其在哺乳動物體內的功能，主要是參與腎小管中水分的再吸收以及離子的運輸，也有研究發現AVP會調節細胞內的酸鹼值。而AVP的同源基因加壓催產素 (AVT)，存在於非哺乳類的脊椎動物及兩生類體內，先前研究已知AVT具有協助魚類適應不同離子濃度與滲透壓環境的功能，但是如何調控魚類體內離子與滲透壓的詳細路徑仍然有待釐清。本研究利用斑馬魚為模式物種，探討AVT在斑馬魚體內調節離子運輸的機制。(為防止研究構想被國外競爭者抄襲，實驗細節內容請與指導教授黃鵬鵬博士連繫) 。總結以上實驗結果，AVT在斑馬魚體內調節離子吸收的功能上具有重要角色。

Arginine vasopressin (AVP), a hormone produced by neurosecretory cells in the posterior pituitary gland of the brain, is one of the neurohypophysial peptides involved in water reabsorption and ion regulation in mammalian kidneys. Its homologous oligopeptide arginine vasotocin (AVT), which is normally expressed in non-mammalian vertebrates and amphibians, has also been proposed to be involved in regulating the ionic/osmotic homeostasis of body fluid in fish; however, the detailed regulatory pathways remain to be clarified. Zebrafish was used as animal models in the present study to test the hypothesis if AVT controls ion transport mechanisms through regulating the expression of the related ion transporters. (To prevent the proposed research ideas from being copied by other competitor labs, please contact Dr. P. P. Hwang for the detailed data). Taken all together into account, AVT appears to play an important role in zebrafish body fluid ionic homeostasis.