請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/5349
標題: | 具多功能之形貌控制金奈米粒子與上轉換奈米粒子複合材料 Multifunctional Nanocomposite of Upconversion Nanoparticle with Different Morphology of Gold Nanoparticles |
作者: | Po-Han Lee 李柏漢 |
指導教授: | 劉如熹 |
關鍵字: | 上轉換奈米粒子,金奈米粒子,二氧化矽殼層,光熱治療, upconversion nanoparticles,gold nanoparticles,silica shell,photothermal therapy, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 上轉換奈米粒子(UCNPs)為一可吸收多顆近紅外光激發光子並轉換為可見光波段光子之發光材料,此特性使其於生醫領域具高度應用潛力。目前上轉換奈米粒子相關之研究可分為直接利用其放光特性應用於生物標記,或者間接轉換釋放之光能於分子檢測及癌症治療之領域,於癌症治療領域主要為結合光敏劑進行光動力療法,利用活性氧物質以致使腫瘤癌細胞死亡。除光動力療法外,上轉換奈米粒子於光熱治療部分之研究仍具許多應用潛力。本研究所合成之多功能奈米複合材料為結合上轉換奈米粒子與不同形貌之金奈米粒子,利用金奈米粒子於特定入射光波長之具表面電漿共振之性質,並透過表面電漿共振所吸收之光能轉換為熱能,搭配具多重放光特性之上轉換奈米粒子提供光能來源以產生熱能致使細胞死亡。本研究利用厚度3 ± 0.5 nm之二氧化矽殼層包覆於大小為19 ± 1.1 nm之上轉換奈米粒子表面,並修飾胺基於以連接大小為3.6 ± 1.11 nm之球狀金奈米粒子(UCNP@SiO2-AuNPs)或徑長比為2.5之棒狀金奈米粒子(UCNP@SiO2-AuNRs),探討不同形貌之金奈米粒子於此複合材料之光熱轉換效率差異。於細胞毒性測試中,本研究所合成之奈米複合材料即便於250 μg/mL高濃度下,對於口腔癌細胞依然具相當低之毒性,可驗證其為低毒性之奈米材料。於光熱治療實驗中,UCNP@SiO2-AuNRs組別相較於UCNP@SiO2-AuNPs及其餘對照組,細胞死亡之數目與範圍皆明顯提升,顯示棒狀金奈米粒子具較強之光熱治療效果。本研究所製備之奈米複合材料,克服傳統金奈米粒子於生物標記之限制,並結合上轉換奈米粒子之螢光成像與金奈米粒子之光熱轉換特性,成功製備具多功能之奈米複合材料。 Upconversion nanoparticles (UCNPs) refer to a highly potent nanomaterial for bioassay and bioimage because it can convert two or more low energy photons to a high energy photon. We demonstrate a nanocomposite consisting of UCNPs and gold nanoparticles as a multifunctional nanomaterial to apply in biomedical application. Noble metallic nanoparticles such as gold nanoparticle have a unique optical property because of the surface plasmon resonance (SPR). Furthermore NaYF4:Yb/Er upconversion nanoparticles emit the photoluminescence from green-to-red which can provide gold nanoparticles inducing photothermal effect. We further used silica coated UCNPs to functionalize amino groups on silica shell to combine 3.5 nm gold nanoparticles (UCNP@SiO2-AuNPs) or gold nanorods (UCNP@SiO2-AuNRs). The size of UCNPs and UCNP@SiO2 are define as 19 ± 1.1 nm and 26 ± 0.5 nm by HRTEM, respectively, and the silica shell is 3 ± 0.5 nm. According to the result of photoluminescence spectra, the intensity of UCNP@SiO2-AuNRs is lower than UCNP@SiO2-AuNPs and this confirm highly energy transfer from UCNP to gold nanorods. In vitro cytotoxicity study, this nanocomposites is nontoxicty to oral cancer cell through the MTT assay even the concentration of nanocomposites approaching 250 μg/mL. The photothermal effect of UCNP@SiO2-AuNRs is demonstrated by irradiating a 980 nm laser and staining with trypan blue, there is huge cell death compare to UCNP@SiO2-AuNPs. In summary, we successfully develop nanocomposites based on UCNPs which reveal obviously photothermal effect and cell imaging to overcome non-fluorescent material like gold nanorods as nanocarriers in biological application. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/5349 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 化學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-103-1.pdf | 5.58 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。