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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 廖文亮(Wen-Liang Liao) | |
| dc.contributor.author | Po-Hao Liao | en |
| dc.contributor.author | 廖柏豪 | zh_TW |
| dc.date.accessioned | 2021-06-15T16:47:24Z | - |
| dc.date.available | 2017-08-20 | |
| dc.date.copyright | 2015-08-20 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-10 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/53150 | - |
| dc.description.abstract | 本研究將靈芝菌絲體固態培養基依相同比例含量且以不同溶劑，包括甲醇、乙醇、醋酸乙酯以及水進行培養基之萃取，將萃取物添加於飼料中探討對點帶石斑魚(Epinephelus coioides)之成長、非特異性免疫效果及血液生化指標的影響。成長檢測的項目包括增重率(Percentage weight gain, PWG)及飼料效率(Feed efficiency, FE)；非特異性免疫檢測的項目包括白血球超氧陰離子(O_2^-)產出率及溶菌酶活性(Lysozyme activity)；血液生化指標檢測的項目包括鹼性磷酸酶(Alkaline phosphatase, ALP)活性、三酸甘油脂(Triglyceride, TG)及膽固醇(Cholesterol, Cho)。
實驗中於飼料添加使用甲醇、乙醇、醋酸乙酯、水萃取及直接添加之靈芝固態培養基1.25%，使用平均體重9.52±1.18 g之點帶石斑魚，每組12尾，進行3重複，飼養35天，第0、21及35天進行秤重，於第35天檢測其非特異性免疫數值及血液生化指標。結果顯示飼料中添加靈芝固態培養基之飼料在增重率上以甲醇最好，飼料效率上以乙醇萃取的成長效果最佳，白血球超氧陰離子(O_2^-)產出率以醋酸乙酯萃取的表現最好，溶菌酶活性以乙醇萃取的活性表現最高，鹼性磷酸酶活性以甲醇萃取的效果較佳，三酸甘油脂及膽固醇皆因飼料添加靈芝固態培養基而有顯著下降(P<0.05)。 | zh_TW |
| dc.description.abstract | In this study, we use different extract solvent including methanol, ethanol, ethyl acetate and water, to extract Ganoderma lucidum mycelium solid medium. Then extraction and solid medium directly was supplemented 1.25% to the dietary, each diet was fed triplicate groups of fish ( initial body weight: 9.52±1.18g ) for 35 days. This research studied the effects of dietary on the growth, non-specific immune parameters and blood biochemical indices of orange-spotted grouper (Epinephelus coioides).
In this experiment, the growth results shows that the percentage of weight gain (203%) of orange-spotted grouper fed with diet extracted by methanol are higher than other groups and the feed converted efficiency (107%) of orange-spotted grouper fed with diet extracted by ethanol are higher than other groups. The non-specific immune responses shows the diet extracted by ethyl acetate (0.436±0.047) is higher production of superoxide anion(O_2^-) than other treatments, the diet extracted by ethanol (49.46±2.00)is higher than other treatments in lysozyme activity. In the blood biochemical indices shows the diet extracted by methanol has higher alkaline phosphatase activity, the concentration of triglyceride and cholesterol in serum are significant decrease (P<0.05) when extracted Ganoderma lucidum mycelium solid medium add to diets compared with control group. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T16:47:24Z (GMT). No. of bitstreams: 1 ntu-104-R02b45013-1.pdf: 2288927 bytes, checksum: 968d50cbad5e30af86cc0e6722fc209d (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 目錄
謝辭 i 中文摘要 iii Abstract iv 表目錄 viii 圖目錄 ix 壹、前言 1 一、全球產業概況 1 貳、文獻整理 4 一、魚種介紹 4 二、全球與台灣之石斑魚養殖現況 4 三、石斑魚養殖困境 5 四、免疫刺激劑(Immunostimulant) 7 五、靈芝介紹 8 六、靈芝型態與生長環境 8 七、靈芝的栽培方法 9 八、靈芝藥理特性與成分 10 九、魚類免疫系統 11 十、血液性狀指標 12 十一、研究動機與目的 14 參、材料方法 15 一、實驗魚種與條件 15 二、飼料製作 15 三、一般成分分析 16 (一)粗蛋白分析(Crude protein, CP) 16 (二)灰分分析(Ash) 16 (三)粗脂質分析(Crude lipid, CL) 17 (四)水分分析 17 四、魚體成長分析 18 五、免疫分析 18 (一)血液、血清、肝臟及頭腎之採集 18 (二)分離白血球 18 (三)超氧陰離子(O_2^-)測定 19 (四)溶菌酶活性測定 19 六、血液生化分析 20 (一)三酸甘油脂(Triglyceride, TG)測定 20 (二)膽固醇(Cholesterol, Cho)測定 20 (三)鹼性磷酸酶(Alkaline phosphatase, ALP) 20 七、統計分析 20 肆、結果 22 一、飼料組成分析 22 二、成長表現 22 三、 魚體組成分析 23 (一)肝體比(hematosomatic index, HSI) 23 (二)脾體比(spleen to body ratio, SBR) 23 四、免疫試驗分析 23 (一)超氧陰離子 23 (二)溶菌酶活性 23 五、 血液性狀指標分析 24 (一)膽固醇(Cholesterol, Cho) 24 (二)三酸甘油脂(Triglyceride, TG) 24 (三)鹼性磷酸酶(Alkaline phosphatase, ALP) 25 伍、討論 26 一、飼料配方 26 二、成長表現 27 三、血液生化分析 28 四、免疫效果分析 28 陸、結論 30 柒、參考文獻 31 附錄 48 | |
| dc.language.iso | zh-TW | |
| dc.subject | 點帶石斑 | zh_TW |
| dc.subject | 菌絲體固態培養基 | zh_TW |
| dc.subject | 血液生化指標 | zh_TW |
| dc.subject | 靈芝 | zh_TW |
| dc.subject | 非特異性免疫 | zh_TW |
| dc.subject | 成長表現 | zh_TW |
| dc.subject | blood biochemical indices | en |
| dc.subject | mycelium solid medium | en |
| dc.subject | orange-spotted grouper | en |
| dc.subject | growth performance | en |
| dc.subject | non-specific immune | en |
| dc.subject | Ganoderma lucidum | en |
| dc.title | 飼料中添加靈芝菌絲體固態培養基萃取物對點帶石斑成長表現與非特異性免疫之影響 | zh_TW |
| dc.title | Effects of crude extract of Ganoderma lucidum mycelium solid medium enriched diet on growth performance and innate immune response in orange-spotted grouper, Epinephelus coioides | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張繼堯,丁雲源,葉信利 | |
| dc.subject.keyword | 靈芝,菌絲體固態培養基,點帶石斑,成長表現,非特異性免疫,血液生化指標, | zh_TW |
| dc.subject.keyword | Ganoderma lucidum,mycelium solid medium,orange-spotted grouper,growth performance,non-specific immune,blood biochemical indices, | en |
| dc.relation.page | 49 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-10 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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