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Title: | 阿拉伯芥中FIN219在鹽害逆境和乾旱逆境的功能性研究 Functional Studies of FIN219 Involved in Drought and Salt Stress in Arabidopsis thaliana |
Authors: | Meng-Hua Sung 宋孟樺 |
Advisor: | 謝旭亮(Hsu-Liang Hsieh) |
Keyword: | 阿拉伯芥,乾旱逆境,鹽害逆境,茉莉酸, FIN219,drought stress,salt stress,ERF, |
Publication Year : | 2016 |
Degree: | 碩士 |
Abstract: | 當植物面臨到生長的逆境時,會藉著賀爾蒙調節生長發育。茉莉酸和乙烯便是目前發現參與多種生物逆境以及非生物逆境的荷爾蒙。FIN219 (FAR-RED INSENSITIVE 219)是一個已被報導參與在茉莉酸生合成的酵素,FIN219可以協助茉莉酸和異白氨酸結合,而接上異白氨酸的茉莉酸為具有活性的茉莉酸,可調節植物的生長及發育。除此之外,FIN219也參與在光訊息傳遞,實驗室前人的研究發現,FIN219可和藍光的接受器隱花色素中的CRY1(cryptochrome 1)在藍光中有互相拮抗的作用。由於目前學界發表的論文中,提到隱花色素的雙突變植株cry1/2對於乾旱具有耐受性,因此FIN219和CRY1之間的拮抗作用暗示FIN219在鹽逆境及乾旱逆境參與的可能。除FIN219和CRY1的關係外,有更多證據顯示FIN219可能會參與在鹽逆境以及乾旱逆境,包括實驗室先前對於FIN219和乙烯之間的交互關係的研究,以及微陣列分析數據顯示FIN219會影響到多個ERF (Ethylene Responsive Factor),都顯示FIN219參與在鹽逆境以及乾旱逆境的可能性。目前我的研究結果顯示,FIN219在鹽逆境的處理之下會改變在細胞中的分布情形,而在特定的鹽逆境處理之下,FIN219會有較低的存活率。在乾旱逆境中,fin219-2 的植株的乾重則會明顯的比野生型的植株重。real-time PCR的結果顯示在鹽逆境之下,fin219-2會影響多個ERF 的表現量。另外在Pull-down assays顯示受FIN219調節的HY5 (Long hypocotyl 5)會和幾個ERF結合。且經過初步的根長測試,erf9和erf13突變體在鹽逆境下的根長受到明顯的抑制。綜合以上所述,推測FIN219可能藉由與CRY1 以及HY5參與在鹽逆境及乾旱逆境之中,我們將進一步的檢驗ERF在鹽逆境及乾旱逆境的表現,來釐清FIN219在這些逆境之中參與的分子機制。 Being immobile, plants use hormones to control their growth when they encounter abiotic stresses. Jasmonates, mainly associated with biotic stresses, also participate in abiotic stress responses. FIN219 (FAR-RED INSENSITIVE 219) is a protein recognized in light signaling pathway, and also discovered to have the ability to conjugate jasmonic acid (JA) with isoleucine (Ile), leading to the formation of JA-Ile. Our lab found that FIN219 and one of blue light receptors-CRY1 (cryptochrome1) antagonized with each other under blue light condition. Previous study revealed that the cry1/2 mutant showed a drought resistance. FIN219 was able to inhibit the CRY1 levels, which suggests the involvement of FIN219 in salt and drought stresses. Moreover, our previous studies indicated that FIN219 played a role in ethylene response and affected several ERF (ethylene responsive factor) transcription factors. Current data showed that FIN219 changed its location under salt stress and the survival rate of fin219-2 seedlings was also reduced under salt stress. However, the growth of fin219-2 was less inhibited compared to Col-0 under osmotic stress. Moreover, FIN219 protein levels decreased under salt and drought stresses. These data indicate that FIN219 may participate in drought and salt responses. Quantitative real-time PCR analyses revealed that some ERFs expressed differently under salt stress between Col-0 and fin219-2. Besides, the levels of HY5 (ELONGATED HYPOCOTYL 5) changed under both stresses. Pull-down assays showed the interaction between HY5 and several ERFs. Further studies revealed that the root lengths of erf9 and erf13 were influenced under salt stress, indicating the involvement of ERF9 and ERF13 in salt stress response. Taken together, the involvement of FIN219 in drought and salt responses may be through negatively and positively regulating cry1 and HY5-ERFs signaling networks. We will further examine the phenotype of ERF mutants under drought and salt stresses in order to confirm the involvement of FIN219 in these stress responses. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51626 |
Fulltext Rights: | 有償授權 |
Appears in Collections: | 植物科學研究所 |
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