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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51248
標題: | 羅漢果皂苷的製備及純化 Preparation and purification of mogrosides from Siraitia grosvenorii |
作者: | Shasha Zhuang 莊莎莎 |
指導教授: | 羅翊禎(Yi-Chen Lo) |
共同指導教授: | 謝淑貞(Shu-Chen Hsieh) |
關鍵字: | 羅漢果皂?,生物轉化,酸水解,HP-20樹脂,乙酸乙酯-水分液萃取, Siraitia grosvenorii,mogroside,biotransformation,acid hydrolysis,HP-20 resin,EA- H2O partition, |
出版年 : | 2016 |
學位: | 碩士 |
摘要: | 羅漢果,學名Siraitia grosvenorii,作為一種傳統中醫藥材常被用來治療感冒、咳嗽、咽喉炎、哮喘、支气管炎和腸胃不適等疾病。各項研究顯示,羅漢果的主要活性成分為一類三萜類皂苷,稱羅漢果皂苷,有如抗氧化、抗病毒、抗癌、抗發炎及抗糖尿病等多種生物活性,在新鮮的羅漢果果實中約占1%,主要以mogroside V的形式存在。羅漢果皂苷還有高甜度、低熱量的特點,可作為天然甜味劑有極強的優勢。由於原料來源及純化技術的限制,過去的研究難以涉及各種羅漢果皂苷間的功效區別。本研究利用不同的方式開發了不同醣基型式羅漢果皂苷之製備方法。用酵母菌Saccharomyces cerevisiae發酵羅漢果果實萃取物,將羅漢果皂苷生物轉化成帶三個醣基的mogroside III E,再以HP-20大孔吸附樹脂管柱對發酵產物進行分離純化,得到純度達54.19%的mogroside III E產品,回收率為72.23%,並成功實現了10倍擴大純化。另外利用化學法將羅漢果萃取物置於2 N鹽酸中在85℃條件下進行酸水解產生皂苷元mogrol,透過乙酸乙酯-水分液萃取法取得純度為13.40%的mogrol產品。本研究中亦利用靈芝菌株ATCC 76537和ATCC 76538將羅漢果皂苷轉化成帶兩個醣基的mogroside II A,進一步延長發酵時間可得到皂苷元mogrol。未來將可利用高純度之各種羅漢果皂苷進行生物利用率等研究。 Lo Han Kuo (LHK), Siraitia grosvenorii, has been used as traditional Chinese herbal medicine to treat colds, cough, sore throats, asthma, bronchitis, stomach and intestinal discomfort. The main bioactive compounds of LHK are triterpenoid saponins, called mogrosides, making up about 1% of fresh fruits, in which mogroside V is the major form. Limited studies demonstrated that mogrosides extracts have various bioactivities, such as antioxidant, anticancer, antivirus, anti-inflammatory and antidiabetes. Studies into the bioactivities of specific mogrosides have been hindered by limit of sources and techniques for purifying these complex chemicals. It has been found that mogroside V could be transformed into other glycosyl-type mogrosides by biologic and chemical methods. The objective of this study is to find the ways to prepare and purify different mogrosides. Mogrosides in LHK extracts were biotransformed into mogroside III E by Saccharomyces cerevisiae, fermentation product was purified by HP-20 resin column to get enriched mogroside III E (54.19%), and the recovery rate for mogroside III E was 72.23%. Mogrol was produced via acid hydrolysis in 2 N HCl at 85℃. Thirteen percents of mogrol was collected with EA-H2O partition of acid hydrolysis products. In addition, mogroside II A and mogrol could be obtained using Ganoderma lucidum ATCC 76537 or ATCC 76538. In conclusion, individual mogroside production can be achieved using various microbial fermentation for future application. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51248 |
全文授權: | 有償授權 |
顯示於系所單位: | 食品科技研究所 |
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