微型化自動介電生物感測器系統之設計與應用

Abstract

微波於生物型感測器領域的應用，可具備即時偵測、樣品無須前處理、操作簡便、體積小與成本低廉等特殊優點，因此而廣受重視。但其需要體積較為龐大且價格昂貴的實驗室儀器協助量測，無法整合運用於一般消費性電子產品中。隨著資訊科技的發展與攜帶式智慧型裝置的普及，微小化的生物感測器已成為未來的發展趨勢。 本研究設計開發新型微波生物感測器系統，以積體電路晶片搭配印刷電路板技術製作實現，透過自動回授控制程序檢測物質於感測器上所造成之容抗與阻抗效應，檢測結果可分別對應至物質介電係數之實部與虛部，具有體積小、低功耗、低工作頻率、即時偵測、免標記、靈敏度高與低成本等優點，並可透過時域積分累積方法，提升檢測結果之訊雜比。 本研究對所提出之生物感測器系統，進行功能性驗證以及物質樣本試驗分析，並且發展自動化使用者介面，增加量測程序之便利性。研究成果可在應用方面快速分析物質成分，並與智慧型裝置等平台結合作為IOT服務應用之工具。

Biosensors that use microwave resonators are compact, easy to operate, low-cost, and capable of real-time monitoring. These advantages, combined with eliminating the need for sample pretreatment, make microwave biosensors the object of widespread attention. However, due to their relatively large size and the expensive laboratory instruments necessary for conducting measurements, they cannot be integrated into general consumer electronic products. With current development in information technology and popularity of portable smart devices, miniature biosensors have become a development trend for the future. In this study, a microwave biosensor system was developed using the technology and process of integrated circuit （IC） and printed circuit board （PCB） to measure, by automatic feedback control, sample-induced capacitance and impedance which correspond respectively to real and imaginary parts of relative permittivity. The device exhibited small size, low power consumption, low operating frequency, label-free detection, high sensitivity, and low cost. In addition, the signal to noise ratio increased with the integration interval. Function validation and sample analysis were performed on the biosensor system. In order to increase the convenience of the detection process, an automated a graphical user interface was developed. In summary, the described system is capable of rapid sample analysis and when integrated into smart device platforms, is a potentially valuable tool for IOT service.