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Title: | 幾丁聚醣調控皮膚黑色素生成與運輸之機制探討 The Modulation Effects of Chitosan on Melanin Synthesis and Melanosomes Uptake |
Authors: | Hsi-wen Chen 陳皙文 |
Advisor: | 楊台鴻(Tai-Horng Young) |
Co-Advisor: | 鄭乃禎(Nai-Chen Cheng) |
Keyword: | 幾丁聚醣,抑制黑色素,美白,B16F10黑色素瘤細胞,人類初代黑色素細胞,人類角質細胞株, Chitosan,hyperpigmentation disorders,depigmentation,melanin,B16F10 melanoma cells,human melanocytes,human keratinocytes HaCaT cell line, |
Publication Year : | 2015 |
Degree: | 碩士 |
Abstract: | 不論在醫學美容或臨床上,抑制皮膚黑色素(melanin)生成皆是極為重要的一項需求,雖然目前已知藥物中已有部分應用於抑制黑色素生成之研究,但仍然無法達到最佳效果。幾丁聚醣(chitosan)為一種具有良好生物相容性及抗菌性的天然材料,此項研究係探討幾丁聚醣對皮膚美白之效果,更針對三個階段(黑色素生成、黑色素小體(melanosome)運送及角質細胞對黑色素小體進行胞吞作用)分別作探討。欲釐清幾丁聚醣之特性對抑制黑色素生成所造成的影響,我們也將不同分子量及不同去乙醯度(degree of deacetylation, D.D.)之幾丁聚醣與B16F10黑色素瘤細胞(melanoma cells)進行培養進而探討在基礎條件或有加黑素細胞激素(alpha melanocyte-stimulation hormone, αMSH)刺激條件下對黑色素生成量進行分析。此外,我們也建立了一個共培養系統研究人類初代黑色素細胞(melanocytes)與人類角質細胞株(HaCaT)欲研究於幾丁聚醣培養環境下之相互作用。研究結果指出不同分子量之幾丁聚醣對於抑制黑色素生成之效果相同,而增加其去乙醯度則會使黑色素生成量顯著減少。並且,幾丁聚醣於前述研究美白效果之三個階段(黑色素生成、黑色素小體運送及角質細胞對黑色素小體進行胞吞作用)皆有顯著抑制的結果。根據結果,由於幾丁聚醣對於皮膚美白有全面性效用,因此我們認為這項研究將可提供一種方法利用幾丁聚醣達到有效抑制黑色素生成之目的。 Decreasing skin pigmentation is an unmet need encountered in various medical or cosmetic conditions. Although some pharmaceutical agents are available for inhibiting melanogenesis, their effects are not ideal. Chitosan, a natural compound extracted from shell fish sources, is a well-known biocompatible and anti-bacterial material. The present research aimed to investigate the skin whitening effects of chitosan on three related processes—melanin synthesis, melanosomes release and melanosomes uptake; and evaluate the possible mechanisms involved. To clarify how the characteristic of chitosan inhibited the melanin synthesis, different molecular weight and different degree of deacetylation (D.D.) of chitosan were added into the culture medium with B16F10 melanoma cells in the basal or αMSH-stimulated condition. Also, our co-culture system is able to study the melanosomes release and uptake between human melanocytes and human keratinocytes HaCaT cell line under the presence of chitosan. Our results have revealed that molecular weight of chitosan would not affect melanogenesis while increasing D.D. of chitosan would enhance the depigmentation effect. Moreover, chitosan can significantly inhibit melanin synthesis and melanosomes release on pigment cells; and lessen melanosomes uptake on keratinocytes. Given the inhibition effect of chitosan on these three processes, we suggest that chitosan is a potential candidate as the treatment of hyperpigmentation disorders. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51001 |
DOI: | 10.6342/NTU201600295 |
Fulltext Rights: | 有償授權 |
Appears in Collections: | 醫學工程學研究所 |
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ntu-104-1.pdf Restricted Access | 2.19 MB | Adobe PDF |
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