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標題: | 組蛋白甲基化轉移酶SUVH4,SUVH5和SUVH6與HDA6共同調控轉座子 The histone methyltransferases SUVH4, SUVH5 and SUVH6 act in concert with HDA6 to regulate transposons |
作者: | Shen-Chi Wang 王燊琪 |
指導教授: | 吳克強(Keqiang Wu) |
關鍵字: | 阿拉伯芥,組蛋白甲基化轉移?,組蛋白去乙醯化?,轉座子, Arabidopsis,histone methyltransferases,histone deacetylases,transposons, |
出版年 : | 2016 |
學位: | 碩士 |
摘要: | SUVH4, SUVH5和SUVH6(SUVH4/5/6)是組蛋白H3賴氨酸9(H3K9)甲基轉移酶,都屬於SET結構域蛋白中SUV(R)亞群。我們發現SUVH4、 SUVH5以及SUVH6都位於細胞核內,並且可以與組蛋白去乙醯化酶HDA6相互作用。通過酵母雙雜交試驗我們發現HDA6的C端及SUVH5的C端對於他們之間的相互作用有關鍵的作用。此外,HDA6的絲氨酸S427的磷酸化會影響它與SUVH5/6的相互作用。通過對RNA-seq的資料分析,我們發現有許多轉座子的表達在hda6-6和suvh4 suvh5 suvh6 (suvh456)的突變體中都有上調,表明SUVH4/5/6和HDA6會共同調控轉座子表達。此外,在had6-6 及suvh456三重突變體中,組蛋白乙醯化水準上升了,而組蛋白H3K9的甲基化水準下降了。同時我們發現在hda6-6 及suvh456三重突變體中,轉座子AT5G59620和AT2G26630的H3K9二甲基化水準下降了。這些結果表明SUVH4/5/6可以通過與HDA6互作改變轉座子的組蛋白甲基化和乙醯化狀態,調控轉座子沉默。 SUVH4, SUVH5 and SUVH6 (SUVH4/5/6) are histone H3 lysine 9 methyltransferases belonging to the SUV(R) group of SET domain proteins. I found that SUVH4, SUVH5 and SUVH6 are all localized in the nucleus and interact with the histone deacetylase HDA6. Both the C-terminal domains of HDA6 and SUVH5 are responsible for their interaction. In addition, phosphorylation on S427 of HDA6 may play an important role for the interaction of HDA6 and SUVH5/6. By the RNA-seq data analysis, we found that a number of transposons are up-regulated in both hda6-6 and suvh4 suvh5 suvh6 (suvh456) mutants, indicating that SUVH4/5/6 and HDA6 act in concert to regulate transposons. Furthermore, the level of histone acetylation is increased but the level of H3 K9 methylation is decreased in hda6-6 and suvh456 triple mutants. The level of H3K9Me2 in the transposons AT5G59620 and AT2G26630 is also decreased in hda6-6 and suvh456. Taken together, these results indicated that HDA6 and SUVH4/5/6 function together to regulate transposon silencing. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50691 |
DOI: | 10.6342/NTU201601012 |
全文授權: | 有償授權 |
顯示於系所單位: | 植物科學研究所 |
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