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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳克強(Keqiang Wu) | |
| dc.contributor.author | Shen-Chi Wang | en |
| dc.contributor.author | 王燊琪 | zh_TW |
| dc.date.accessioned | 2021-06-15T12:52:54Z | - |
| dc.date.available | 2021-08-02 | |
| dc.date.copyright | 2016-08-02 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-07-19 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50691 | - |
| dc.description.abstract | SUVH4, SUVH5和SUVH6(SUVH4/5/6)是組蛋白H3賴氨酸9(H3K9)甲基轉移酶，都屬於SET結構域蛋白中SUV(R)亞群。我們發現SUVH4、 SUVH5以及SUVH6都位於細胞核內，並且可以與組蛋白去乙醯化酶HDA6相互作用。通過酵母雙雜交試驗我們發現HDA6的C端及SUVH5的C端對於他們之間的相互作用有關鍵的作用。此外，HDA6的絲氨酸S427的磷酸化會影響它與SUVH5/6的相互作用。通過對RNA-seq的資料分析，我們發現有許多轉座子的表達在hda6-6和suvh4 suvh5 suvh6 （suvh456）的突變體中都有上調，表明SUVH4/5/6和HDA6會共同調控轉座子表達。此外，在had6-6 及suvh456三重突變體中，組蛋白乙醯化水準上升了，而組蛋白H3K9的甲基化水準下降了。同時我們發現在hda6-6 及suvh456三重突變體中，轉座子AT5G59620和AT2G26630的H3K9二甲基化水準下降了。這些結果表明SUVH4/5/6可以通過與HDA6互作改變轉座子的組蛋白甲基化和乙醯化狀態，調控轉座子沉默。 | zh_TW |
| dc.description.abstract | SUVH4, SUVH5 and SUVH6 (SUVH4/5/6) are histone H3 lysine 9 methyltransferases belonging to the SUV(R) group of SET domain proteins. I found that SUVH4, SUVH5 and SUVH6 are all localized in the nucleus and interact with the histone deacetylase HDA6. Both the C-terminal domains of HDA6 and SUVH5 are responsible for their interaction. In addition, phosphorylation on S427 of HDA6 may play an important role for the interaction of HDA6 and SUVH5/6. By the RNA-seq data analysis, we found that a number of transposons are up-regulated in both hda6-6 and suvh4 suvh5 suvh6 (suvh456) mutants, indicating that SUVH4/5/6 and HDA6 act in concert to regulate transposons. Furthermore, the level of histone acetylation is increased but the level of H3 K9 methylation is decreased in hda6-6 and suvh456 triple mutants. The level of H3K9Me2 in the transposons AT5G59620 and AT2G26630 is also decreased in hda6-6 and suvh456. Taken together, these results indicated that HDA6 and SUVH4/5/6 function together to regulate transposon silencing. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T12:52:54Z (GMT). No. of bitstreams: 1 ntu-105-R03b42033-1.pdf: 3636608 bytes, checksum: 0f882e5213553c096f9a2727c2e1a19a (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 致謝 I
中文摘要 II Abstract III List of Figures VII List of Supplementary Figures IX List of Supplementary Tables X List of Abbreviations XI 1. Introduction 1 1.1 SET domain proteins in Arabidopsis 1 1.2 Function of SET domain proteins in Arabidopsis 3 1.3 Histone deacetylases in Arabidopsis 4 1.4 Crosstalk of histone methylation and acetylation 6 2. Materials and Methods 8 2.1 Plant materials 8 2.2 Quick DNA extraction 8 2.3 RNA isolation 9 2.4 DNase treatment 10 2.5 Quantitative RT-PCR analysis 11 2.6 Bimolecular Fluorescence Complementation (BiFC) assays 12 2.7 Yeast two-hybrid assays 15 2.8 Transfection of tobacco or GVG leaves by Agrobacterium (For Co-Immunoprecipitation) 17 2.9 Histone modification assay 18 2.10 Chromatin immunoprecipitation assays 21 3. Results 29 3.1 The phylogenic tree of SU（VAR）3-9 group proteins 29 3.2 Expression and localization of SUVH4, SUVH5 and SUVH6 29 3.3 SUVH5 interacted with the RPD3-type HDACs 30 3.4 Identification of interaction domains of HDA6 and SUVH5 31 3.5 Phosphorylation on S427 of HDA6 affected its interaction with SUVH5 and SUVH6 31 3.6 Identification of homozygous T-DNA insertion mutants of SUVH4, SUVH5, SUVH6 32 3.7 HDA6 may act in concert with SUVH4/5/6 to regulate transposons 33 3.8 The histone modification level of transposons was altered in the suvh456 triple mutant 34 4. Discussion 36 4.1 SUVH4/5/6 are involved in seed dormancy and germination 36 4.2 HDA6 phosphorylation affects its interaction with SUVH5/6 36 4.3 SUVH4/5/6 act together with HDA6 to regulate transposons 37 Figures 40 References 63 Supplementary Figures 63 Supplementary Tables 78 | |
| dc.language.iso | en | |
| dc.title | 組蛋白甲基化轉移酶SUVH4，SUVH5和SUVH6與HDA6共同調控轉座子 | zh_TW |
| dc.title | The histone methyltransferases SUVH4, SUVH5 and SUVH6 act in concert with HDA6 to regulate transposons | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林讚標,鄭貽生,涂世隆,陳柏仰 | |
| dc.subject.keyword | 阿拉伯芥,組蛋白甲基化轉移?,組蛋白去乙醯化?,轉座子, | zh_TW |
| dc.subject.keyword | Arabidopsis,histone methyltransferases,histone deacetylases,transposons, | en |
| dc.relation.page | 93 | |
| dc.identifier.doi | 10.6342/NTU201601012 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-07-19 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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