脂膜筏及Caveolin藉由Src引導纖維母細胞於電場中之方向性移動

Abstract

電趨性為細胞受到電場影響而表現出與電場方向平行移動的現象。先前的研究指出脂膜筏──一種細胞膜上的微結構──藉由調控integrin或是caveolin-1影響細胞的移動方向。也發現細胞在交流電及直流電中有相反方向的移動。本研究指出脂膜筏僅會在低頻率的交流電中有方向性的移動，且在具有不同方向移動表現型的細胞中，脂膜筏在細胞中的偏向也有不同。且脂膜筏及caveolin-1皆可藉由改變在細胞中的偏向來調控Src 在細胞中的偏向來引導細胞在電場中移動的方向。我們也發現PI3K以藉由改變Src 在細胞中的偏向參與其中。這些結果提供的兩種面向探討脂膜筏如何改變細胞的方向性：脂膜筏在細胞膜表面上的移動方向，以及其下游的傳導途徑。

Galvanotaxis is a phenomenon where cell migration directed by electric fields (EFs). Previous studies revealed that the redistribution of lipid raft guides cell directional motility by regulating integrin or caveolin-1, and cells has opposite directionality between AC and DC. This study indicates that lipid raft can be polarized under in 50Hz of alternative current, and the redistribution of lipid raft can be different in different migration phenotype cells. Lipid raft and caveolin-1 polarization can guide cell directional migration in EF by redistribute Src polarization. PI3K also involved in the pathway by affecting Src polarization. These results clarified how lipid raft polarization change cell migration in EF in two aspects: the position lipid rafts distribute in different situations, and the downstream pathways.