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標題: | MYB3及GRP2在阿拉伯芥幼苗光型態發生之功能研究 Functional study of MYB3 and GRP2 in photomorphogenic development of Arabidopsis seedlings |
作者: | Jia Ku 顧珈 |
指導教授: | 吳克強(Keqiang Wu) |
關鍵字: | 組蛋白修飾,組蛋白去乙醯基酶HDA15,MYB3,GRP2,光型態發生,下胚軸延長, Histone deacetylation,HDA15,MYB3,GRP2,photomorphogenesis,hypocotyl elongation, |
出版年 : | 2020 |
學位: | 碩士 |
摘要: | 組蛋白去乙醯基酶藉由表觀遺傳修飾影響植物生長發育。在先前的研究中得知阿拉伯芥組蛋白去乙醯基酶HDA15可與許多的蛋白質有交互作用。這些蛋白質包括了轉錄因子MYB3和RNA結合蛋白GRP2。進一步分析了這些HDA15互作蛋白質MYB3和GRP2的功能。先前的研究指出HDA15會參與調控光相關的葉綠素生合成和下胚軸延長,在逆境反應中也有參與。我的研究發現轉錄因子MYB3可以與HDA15互作,在黑暗中負調節葉綠素的生物合成及在遠紅光下調控下胚軸伸長。另一方面,GRP2也可以與HDA15相互作用,並且參與葉綠素的生合成、下胚軸延長和非生物逆境反應。綜上所述,我的研究發現MYB3和GRP2在光形態形成中發揮重要作用。 Histone deacetylases (HDACs) are an important class of global transcriptional regulators that play crucial roles in plant development, defense, and adaptation. Previous studies indicated that HISTONE DEACETYLASE 15 (HDA15) can interact with many proteins including the transcription factor MYB3 and the RNA binding protein GRP2. We further analyzed the functions of MYB3 and GRP2 by identifying and characterizing of their T-DNA insertion mutants. I found that MYB3 interacts with HDA15 and negatively regulates chlorophyll biosynthesis in darkness and hypocotyl elongation under far-red light. In addition, GRP2 can also interact with HDA15 and negatively regulate chlorophyll biosynthesis in darkness and hypocotyl elongation under far-red light. Furthermore, GRP2 also positively regulates abiotic stress responses. Taken together, our studies indicate that both MYB3 and GRP2 play essential roles in regulating photomorphogenic development of Arabidopsis seedlings. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48976 |
DOI: | 10.6342/NTU202003187 |
全文授權: | 有償授權 |
顯示於系所單位: | 植物科學研究所 |
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