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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 吳克強(Keqiang Wu) | |
dc.contributor.author | Jia Ku | en |
dc.contributor.author | 顧珈 | zh_TW |
dc.date.accessioned | 2021-06-15T11:12:48Z | - |
dc.date.available | 2020-08-21 | |
dc.date.copyright | 2020-08-21 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-08-17 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48976 | - |
dc.description.abstract | 組蛋白去乙醯基酶藉由表觀遺傳修飾影響植物生長發育。在先前的研究中得知阿拉伯芥組蛋白去乙醯基酶HDA15可與許多的蛋白質有交互作用。這些蛋白質包括了轉錄因子MYB3和RNA結合蛋白GRP2。進一步分析了這些HDA15互作蛋白質MYB3和GRP2的功能。先前的研究指出HDA15會參與調控光相關的葉綠素生合成和下胚軸延長,在逆境反應中也有參與。我的研究發現轉錄因子MYB3可以與HDA15互作,在黑暗中負調節葉綠素的生物合成及在遠紅光下調控下胚軸伸長。另一方面,GRP2也可以與HDA15相互作用,並且參與葉綠素的生合成、下胚軸延長和非生物逆境反應。綜上所述,我的研究發現MYB3和GRP2在光形態形成中發揮重要作用。 | zh_TW |
dc.description.abstract | Histone deacetylases (HDACs) are an important class of global transcriptional regulators that play crucial roles in plant development, defense, and adaptation. Previous studies indicated that HISTONE DEACETYLASE 15 (HDA15) can interact with many proteins including the transcription factor MYB3 and the RNA binding protein GRP2. We further analyzed the functions of MYB3 and GRP2 by identifying and characterizing of their T-DNA insertion mutants. I found that MYB3 interacts with HDA15 and negatively regulates chlorophyll biosynthesis in darkness and hypocotyl elongation under far-red light. In addition, GRP2 can also interact with HDA15 and negatively regulate chlorophyll biosynthesis in darkness and hypocotyl elongation under far-red light. Furthermore, GRP2 also positively regulates abiotic stress responses. Taken together, our studies indicate that both MYB3 and GRP2 play essential roles in regulating photomorphogenic development of Arabidopsis seedlings. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T11:12:48Z (GMT). No. of bitstreams: 1 U0001-1308202004135000.pdf: 5505882 bytes, checksum: 29ef113b6e6679d6a8d40b4023197baa (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | CONTENTS 口試委員會審定書 # 誌謝 i 摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES vii LIST OF SUPPLEMENTARY FIGURES viii LIST OF TABLES ix LIST OF ABBREVIATIONS x Chapter 1 Introduction 1 1.1 Light regulation of plant development 1 1.2 Histone deacetylases 2 1.3 MYB transcription factors 4 1.4 GRP RNA binding proteins 6 Chapter 2 Materials and Methods 9 2.1 Plant materials 9 2.2 Plasmid construction 10 2.3 Generating MYB3-GFP and GRP2-GFP transgenic plants 11 2.4 Quick DNA extraction 13 2.5 RNA Extraction, DNase treatment and reverse transcription 13 2.6 Real-time PCR analysis 18 2.7 Chromatin immunoprecipitation assays 18 2.8 Transfection of tobacco leaves by Agrobacterium 25 2.9 Protoplast transformation 26 2.10 Protochlorophyllide determination assays 30 Chapter 3 Results 31 3.1 Expression and subcellular localization of MYB3 and GRP2 31 3.2 Identification of myb3 and grp mutants 31 3.3 HDA15 interacts with MYB3 and GRP2 in vivo 32 3.4 MYB3 and GRP2 regulate protochlorophyllide biosynthesis 33 3.5 The hypocotyl elongation phenotypes of hda15, myb3 and grp2 34 3.6 Gene expression involved in photosynthesis and hypocotyl elongation 34 3.7 Levels of histone H4 acetylation on GUN5 and PSBQ loci in hda15 and myb3 mutant plants 36 3.8. grp2-1 and grp2-2 are hypersensitive to NaCl and ABA in seed germination 36 Chapter 4 Discussion 38 4.1 MYB3 participates in chlorophyll biosynthesis and photosynthesis 38 4.2 MYB3 affects hypocotyl elongation under far red-light conditions 40 4.3 GRP2 participates in hypocotyl elongation and stress response 41 FIGURES 44 SUPPLEMENTARY FIGURES 57 TABLES 67 REFERENCES 72 | |
dc.language.iso | en | |
dc.title | MYB3及GRP2在阿拉伯芥幼苗光型態發生之功能研究 | zh_TW |
dc.title | Functional study of MYB3 and GRP2 in photomorphogenic development of Arabidopsis seedlings | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 謝旭亮(Hsu-Liang Hsieh),鄭貽生(Yi-Sheng Cheng),張英峯(Ing-Feng Chang),李金美(Chin-Mei Lee) | |
dc.subject.keyword | 組蛋白修飾,組蛋白去乙醯基酶HDA15,MYB3,GRP2,光型態發生,下胚軸延長, | zh_TW |
dc.subject.keyword | Histone deacetylation,HDA15,MYB3,GRP2,photomorphogenesis,hypocotyl elongation, | en |
dc.relation.page | 76 | |
dc.identifier.doi | 10.6342/NTU202003187 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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