請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48966完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 呂廷璋 | |
| dc.contributor.author | Jyun-Cyuan Ke | en |
| dc.contributor.author | 柯鈞銓 | zh_TW |
| dc.date.accessioned | 2021-06-15T11:12:33Z | - |
| dc.date.available | 2021-08-25 | |
| dc.date.copyright | 2016-08-25 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-21 | |
| dc.identifier.citation | Aasen, A. J.; Lauer, W. M.; Holman, R. T., Mass spectrometry of triglycerides II. specifically deuterated triglycerides and elucidation of fragmentation mechanisms. Lipids 1970, 5, 869-877.
Amaral, J. S.; Cunha, S. C.; Alves, M. R.; Pereira, J. A.; Seabra, R. M.; Oliveira, B. P. P., Triacylglycerol composition of walnut (Juglans regia L.) cultivars: Characterization by HPLC-ELSD and chemometrics. J. Agric. Food Chem. 2004, 52, 7964-7969. Asperger, A.; Efer, R.; Koal, T.; Engewald, W., On the signal response of various pesticides in electrospray and atmospheric pressure chemical ionization depending on the flow-rate of eluent applied in liquid chromatography-tandem mass spectrometry. J. Chromatogr. A 2001, 937, 65-72. Ayorinde, F. O.; Eribo, B. E.; Balan, K. V.; Johnson, J. H.; Wan, L. W., Determination of major triacylglycerol components of polyunsaturated specialty oils using matrix-assisted laser desorption ionization time-of-fight mass spectrometry. Rapid Commun. Mass. Spectrom. 1999, 13, 937-942. Bailey, A. E., Bailey's industrial oil & fat products. fifth ed.; Wiley: New York, 1996; Vol. 2. Bailey, A. E., Bailey's industrial oil & fat products. sixth ed.; John Wiley & Sons: Hoboken, N.J., 2005; Vol. 2. Bansal, U. K.; Satija, D. R.; Ahuja, K. L., Oil composition of diverse groundnut (Arachis hypogaea L) genotypes in relation to different environments. J. Sci.Ffood Agric. 1993, 63, 17-19. Belizt, H. D.; Grosch, W.; Schieberle, P., Food Chemistry. 4th ed.; Springer: New Work, 2009. Beppu, F.; Nagai, T.; Yoshinaga, K.; Mizobe, H.; Kojima, K.; Gotoh, N., Quantification of triacylglycerol molecular species in cocoa butter using high-performance liquid chromatography equipped with nano quantity analyte detector. J. Oleo. Sci. 2013, 62, 789-794. Brockerh.H, A Stereospecific analysis of triglycerides. J. Lipid Res. 1965, 6, 10-15. Byrdwell, W. C.; Emken, E. A., Analysis of triglycerides using atomspheric-pressure chemical ionization mass-spectrometry. Lipids 1995, 30, 173-175. Byrdwell, W. C.; Neff, W. E., Non-volatile products of triolein produced at frying temperatures characterized using liquid chromatography with online mass spectrometric detection. J. Chromatogr. A 1999, 852, 417-432. Byrdwell, W. C.; Neff, W. E.; List, G. R., Triacylglycerol analysis of potential margarine base stocks by high-performance liquid chromatography with atmospheric pressure chemical ionization mass spectrometry and flame ionization detection. J. Agric. Food Chem. 2001, 49, 446-457. Byrdwell, W. C.; Neff, W. E., Dual parallel electrospray ionization and atmospheric pressure chemical ionization mass spectrometry (MS), MS/MS and MS/MS/MS for the analysis of triacylglycerols and triacylglycerol oxidation products. Rapid Commun. Mass. Spectrom. 2002, 16, 300-319. Cai, S. S.; Syage, J. A., Comparison of atmospheric pressure photoionization, atmospheric pressure chemical ionization, and electrospray ionization mass spectrometry for analysis of lipids. Anal. Chem. 2006, 78, 1191-1199. Canabate-Diaz, B.; Carretero, A. S.; Fernandez-Gutierrez, A.; Vega, A. B.; Frenich, A. G.; Vidal, J. L. M.; Martos, J. D., Separation and determination of sterols in olive oil by HPLC-MS. Food. Chem. 2007, 102, 593-598. Carelli, A. A.; Cert, A., Comparative-study of the determination of triacylglycerol in vegetable-oils using chromatographic techniques. J. Chromatogr 1993, 630, 213-222. Che Man, Y. B.; Tan, C. P., Lipids for functional foods and nutraceuticals. Oily Press: Bridgwater, UK, 2003. Cheng, C. F.; Gross, M. L., Complete structural elucidation of triacylglycerols by tandem sector mass spectrometry. Anal. Chem. 1998, 70, 4417-4426. Contarini, G.; Povolo, M.; Bonfitto, E.; Berardi, S., Quantitative analysis of sterols in dairy products: experiences and remarks. Int. Dairy J. 2002, 12, 573-578. Cunha, S. C.; Oliveira, M., Discrimination of vegetable oils by triacylglycerols evaluation of profile using HPLC/ELSD. Food. Chem. 2006, 95, 518-524. de la Mora, J. F.; Van Berkel, G. J.; Enke, C. G.; Cole, R. B.; Martinez-Sanchez, M.; Fenn, J. B., Electrochemical processes in electrospray ionization mass spectrometry - Discussion. J. Mass Spectrom. 2000, 35, 939-952. Dobson, G.; Christie, W. W.; Nikolovadamyanova, B., Silver ion chromatography of lipids and fatty acids. J. Chromatogr., Biomed. Appl. 1995, 671, 197-222. Dorschel, C. A., Characterization of the TAG of peanut oil by electrospray LC-MS-MS. J. Am. Oil Chem. Soc. 2002, 79, 749-753. Duffin, K. L.; Henion, J. D.; Shieh, J. J., Electrospary and tandem mass-spectrometric characterization of acylglycerol mixtures that are dissolved in nonpolar-solvents. Anal. Chem. 1991, 63, 1781-1788. Dunlap, F. G.; White, P. J.; Pollak, L. M., Fatty-acid composition of oil from exotic corn breeding materials. J. Am. Oil Chem. Soc. 1995, 72, 989-993. El-Hamdy, A. H.; Perkins, E. G., High performance reversed phase chromatography of natural triglyceride mixtures: critical pair separation. J. Am. Oil Chem. Soc. 1981, 58, 867-872. Endo, Y.; Ohta, A.; Kido, H.; Kuriyama, M.; Sakaguchi, Y.; Takebayashi, S.; Hirai, H.; Murakami, C.; Wada, S., Determination of triacylglycerol composition in vegetable oils using high-performance liquid chromatography: a collaborative study. J. Oleo. Sci. 2011, 60, 451-456. Fauconnot, L.; Hau, J.; Aeschlimann, J. M.; Fay, L. B.; Dionisi, F., Quantitative analysis of triacylglycerol regioisomers in fats and oils using reversed-phase high-performance liquid chromatography and atmospheric pressure chemical ionization mass spectrometry. Rapid Commun. Mass. Spectrom. 2004, 18, 218-224. Fenn, J. B.; Mann, M.; Meng, C. K.; Wong, S. F.; Whitehouse, C. M., Electrospray ionization for mass-spectrometry of large biomolecules. Science 1989, 246, 64-71. Fenn, J. B.; Mann, M.; Meng, C. K.; Wong, S. F.; Whitehouse, C. M., Electrospray ionization-principles and practice. Mass Spectrom. Rev. 1990, 9, 37-70. Fennema, O. R., Fennema's Food Chemistry 3rd ed.; Marcel Dekker: New York, 1996. Goiffon, J. P.; Reminiac, C.; Furon, D., High-pressure liquid-chromatography application for fatty triglycerides analysis .2. – retention parameters of triglycerides. Revue Francaise Des Corps Gras 1981, 28, 199-207. Gotoh, N.; Aoki, T.; Nakayasu, K.; Tokairin, S.; Noguchi, N.; Wada, S., Quantification method for triglyceride molecular species in fish oil with high performance liquid chromatography - ultraviolet detector - evaporative light scattering detector. J. Oleo. Sci. 2006, 55, 457-463. Gunstone, F. D., Vegetable oils in food technology. Blackwell: Oxford, U.K., 2002; p 59-97. Hokes, J. C.; Worthington, R. E., Structure of peanut oil triacylglycerols from cultivars of diverse genetic background. J. Am. Oil Chem. Soc. 1979, 56, 953-956. Horning, E. C.; Horning, M. G.; Carroll, D. I.; Dzidic, I.; Stillwel.Rn, New picogram detection system based on a mass-spectrometer with an external ionization source at atmospheric-pressure. Anal. Chem. 1973, 45, 936-943. Hvattum, E., Analysis of triacylglycerols with non-aqueous reversed phase liquid chromatography and positive ion electrospray tandem mass spectrometry. Rapid Commun. Mass. Spectrom. 2001, 15, 187-190. Jham, G. N.; Muller, H. V.; Cecon, P., Triacylglycerol molecular species variation in stored coffee beans determined by reverse-high-performance liquid chromatography/refractive index detector. J. Stored Prod. Res. 2008, 44, 82-89. Kallio, H.; Currie, G., Analysis of low erucic-acid turnip rapeseed oil (Brassica-Campestris) by negative-ion chemical ionization tandem mass-spectrometry - a method giving information on the fatty-acid composition in positions sn-2 and sn-1/3 of triacylglycerols. Lipids 1993, 28, 207-215. Kalo, P. J.; Ollilainen, V.; Rocha, J. M.; Malcata, F. X., Identification of molecular species of simple lipids by normal phase liquid chromatography-positive electrospray tandem mass spectrometry, and application of developed methods in comprehensive analysis of low erucic acid rapeseed oil lipids. Int. J. Mass Spectrom. 2006, 254, 106-121. Laakso, P., Mass spectrometry of triacylglycerols. Eur. J. Lipid. Sci. Tech. 2002, 104, 43-49. Lauer, W. M.; Aasen, A. J.; Graff, G.; Holman, R. T., Mass spectrometry of triglycerides I. structural effects. Lipids 1970, 5, 861-&. Lembcke, J.; Ceglarek, U.; Fiedler, G. M.; Baumann, S.; Leichtle, A.; Thiery, J., Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS. J. Lipid Res. 2005, 46, 21-26. Mangos, T. J.; Jones, K. C.; Foglia, T. A., Normal-phase high performance liquid chromatographic separation and characterization of short- and long-chain triacylglycerols. Chromatographia. 1999, 49, 363-368. Marzilli, L. A.; Fay, L. B.; Dionisi, F.; Vouros, P., Structural characterization of triacylglycerols using electrospray ionization-MSn ion-trap MS. J. Am. Oil Chem. Soc. 2003, 80, 195-202. Matthäus, B.; Őzcan, M. M.; , Determination of fatty acid, tocopherol, sterol contents and 1,2- and 1,3-diacylglycerols in four different virgin olive oil. J. Food Process. Technol. 2011, 2, 1-4. McAnoy, A. M.; Wu, C. C.; Murphy, R. C., Direct qualitative analysis of triacylglycerols by electrospray mass spectrometry using a linear ion trap. J. Am. Soc. Mass Spectrom. 2005, 16, 1498-1509. Mo, S. Y.; Dong, L. L.; Hurst, W. J.; van Breemen, R. B., Quantitative analysis of phytosterols in edible oils using APCI liquid chromatography-tandem mass spectrometry. Lipids 2013, 48, 949-956. Momchilova, S.; Tsuji, K.; Itabashi, Y.; Nikolova-Damyanova, B.; Kuksis, A., Resolution of triacylglycerol positional isomers by reversed-phase high-performance liquid chromatography. J. Sep. Sci 2004, 27, 1033-1036. Mottram, H. R.; Woodbury, S. E.; Evershed, R. P., Identification of triacylglycerol positional isomers present in vegetable oils by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry. Rapid Commun. Mass. Spectrom. 1997, 11, 1240-1252. Myher, J. J.; Kuksis, A., Electrospray-MS for lipid identification. INFORM 1995, 6, 1068-1072. Neff, W. E.; Byrdwell, W. C., Soybean oil triacylglycerol analysis by reversed-phase high-performance liquid-chromatography coupled with atomspheric-pressure chemical-ionization mass spectrometry. J. Am. Oil Chem. Soc. 1995, 72, 1185-1191. Plattner, R. D.; Spencer, G. F.; Kleiman, R., Triglyceride separation by reverse phase high performance liquid chromatography. J. Am. Oil Chem. Soc. 1977, 54, 511-515. Podlaha, O.; Toregard, B., A system for identification of triglycerides in reversed phase HPLC chromatograms based on equivalent carbon numbers. J. High Resolut. Chromatogr. Chromatogr. Commun. 1982, 5, 553-558. Rohman, A.; Triyana, K.; Sismindari; Erwanto, Y., Differentiation of lard and other animal fats based on triacylglycerols composition and principal component analysis. Int. Food Res. J. 2012, 19, 475-479. Rozenberg, R.; Ruibal-Mendieta, N. L.; Petitjean, G.; Cani, P.; Delacroix, D. L.; Delzenne, N. M.; Meurens, M.; Quetin-Leclercq, J.; Habib-Jiwan, J. L., Phytosterol analysis and characterization in spelt (Triticum aestivum ssp spelta L.) and wheat (T aestivum L.) lipids by LC/APCI-MS. J. Cereal. Sci. 2003, 38, 189-197. Rutan, S. C.; Carr, P. W.; Cheong, W. J.; Park, J. H.; Snyder, L. R., Re-evaluation of the solvent triangle and comparison to solvatochromic based scales of solvent strength and selectivity. J. Chromatogr 1989, 463, 21-37. Sanders, T. H., Peanut triacylglycerols - effect of season and production location. J. Am. Oil Chem. Soc. 1982, 59, 346-351. Sjovall, O.; Kuksis, A.; Marai, L.; Myher, J. J., Elution factors of synthetic oxotriacylglycerols as an aid in identification of peroxidized natural triacylgylcerols by reverse-phase high-performance liquid chromatography with electrospray mass spectrometry. Lipids 1997, 32, 1211-1218. Smith, R. D.; Loo, J. A.; Edmonds, C. G.; Barinaga, C. J.; Udseth, H. R., New developments in biochemical mass spectrometry-electrospray ionization. Anal. Chem. 1990, 62, 882-899. Snyder, L. R.; Carr, P. W.; Rutan, S. C., Solvatochromically based solvent-selectivity triangle. J. Chromatogr. A 1993, 656, 537-547. Tarandjiiska, R. B.; Marekov, I. N.; NikolovaDamyanova, B. M.; Amidzhin, B. S., Determination of triacylglycerol classes and molecular species in seed oils with high content of linoleic and linolenic fatty acids. J. Sci.Ffood Agric. 1996, 72, 403-410. Tracy, M. L.; Wang, L.; Liu, X.; Lopez, L., Analysis for triglycerides, diglycerides, and monoglycerides by high performance liquid chromatography (HPLC) using 2.2 µm rapid separation C18 columns with alternative solvent systems and mass spectrometry. In Application Note, Dionex Corporation: USA, 2010. Turon, F.; Bachain, P.; Caro, Y.; Pina, M.; Graille, J., A direct method for regiospecific analysis of TAG using alpha-MAG. Lipids 2002, 37, 817-821. Tzompa-Sosa, D. A.; van Aken, G. A.; van Hooijdonk, A. C. M.; van Valenberg, H. J. F., Influence of C16:0 and long-chain saturated fatty acids on normal variation of bovine milk fat triacylglycerol structure. J. Dairy Sci. 2014, 97, 4542-4551. van der Klift, E. J. C.; Vivo-Truyols, G.; Claassen, F. W.; van Holthoon, F. L.; van Beek, T. A., Comprehensive two-dimensional liquid chromatography with ultraviolet, evaporative light scattering and mass spectrometric detection of triacylglycerols in corn oil. J. Chromatogr. A 2008, 1178, 43-55. Verleyen, T.; Forcades, M.; Verhe, R.; Dewettinck, K.; Huyghebaert, A.; De Greyt, W., Analysis of free and esterified sterols in vegetable oils. J. Am. Oil Chem. Soc. 2002, 79, 117-122. Vieitez, I.; Irigaray, B.; Callejas, N.; Gonzalez, V.; Gimenez, S.; Arechavaleta, A.; Grompone, M.; Gambaro, A., Composition of fatty acids and triglycerides in goat cheeses and study of the triglyceride composition of goat milk and cow milk blends. J. Food Compos. Anal. 2016, 48, 95-101. White, P. J., Fatty Acids in Foods and their Health Implications. 3rd ed.; 2007. Winkler-Moser, J. K., Gas Chromatographic Analysis of Plant Sterols. In Lipid Analysis, AOCS Lipid Library, 2011. Yao, Y. P.; Zhao, G. Z.; Xiang, J. Y.; Zou, X. Q.; Jin, Q. Z.; Wang, X. G., Lipid composition and structural characteristics of bovine, caprine and human milk fat globules. Int. Dairy J. 2016, 56, 64-73. Zeb, A.; Murkovic, M., Analysis of triacylglycerols in refined edible oils by isocratic HPLC-ESI-MS. Eur. J. Lipid. Sci. Tech. 2010, 112, 844-851. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48966 | - |
| dc.description.abstract | 摘要
三酸甘油酯為食用油中最主要的成分，不同來源油脂除脂肪酸組成外，其脂肪醯基分布在甘油酯上的位置亦為重要的特徵。本研究目的以液相層析串聯質譜法分析固醇類化合物並建立三酸甘油酯組成及脂肪酸分布資訊。逆相液相層析採用 Accucore C30 管柱，整體分析時間為25分鐘，可將三酸甘油酯依據碳當量數 (equivalent carbon number, ECN) 將其分群並能將部分具有相同碳當量數的三酸甘油酯異構物分離，經電噴灑游離後，三酸甘油酯的銨加成離子([M+NH4]+)為其特徵離子訊號，二次質譜分析可辨別脂肪酸的組成及甘油酯sn-2位置上脂肪酸的資訊。以市售食用油中三酸甘油酯的組成可建構出油品的指紋特徵，以主成分分析可將市售食用油做分群，另外，以液相層析串聯大氣壓游離質譜法分析食用油中固醇類化合物，動物油主要特徵為膽固醇，市售油植物固醇以β-sitosterol、stigmasterol與campesterol為主，玄米油中另外含有酚酸酯化態固醇(米糠醇)，而麻油中的seasmin、seasmolin，橄欖油中的squalene、苦茶油中的β-ayrmin為具有特徵的化合物，可做為食用油特徵辨別的依據。 | zh_TW |
| dc.description.abstract | Triacylglycerols are the major component of edible oils. In addition to fatty acid composition, distribution pattern is an essential characteristics for each botanic source of edible oil. An ultra-performance liquid chromatography-electrospray tandem mass spectrometry was developed for fingerprint recognition of edible oils. Triacylglycerols were separated and grouped according their equivalent carbon number (ECN). The MS data recorded for ammonium adducts ([M+NH4]+) of triacylglycerols and the signals of onium ion of diacylglycerol in MS2 were used to differentiate the fatty acid on the sn-2 position of original triacylglycerols. The major phytosterols in commercial edible oil were -sitosterol, stigmasterol and campesterol. The -oryzanol, ferulate esters were found in rice bran oil. Seasmin、seasmolin in sesame oil, squalene in olive oil and β-ayrmin in camellia oil can be used as characteristic compounds. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T11:12:33Z (GMT). No. of bitstreams: 1 ntu-105-R03641014-1.pdf: 8646530 bytes, checksum: a43d3bb85a7637e1759e31b3b3b46a7a (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 壹、 前言 1
貳、 文獻回顧 2 第一節、 食用油 2 一、 簡介 2 二、 組成及分類 2 第二節、 三酸甘油酯 4 一、 簡介 4 二、 化學結構 4 第三節、 三酸甘油酯的分析 6 一、 液相層析法 6 二、 檢測器 10 三、 三酸甘油酯的游離及斷裂機制 11 四、 立體特異性分析 15 第四節、 固醇 17 一、 結構 17 二、 固醇的分析 18 參、 研究目的與實驗架構 20 肆、 材料與方法 21 第一節、 實驗材料 21 第二節、 藥品與試劑 21 一、 標準品 21 二、 化學藥品 21 第三節、 儀器設備 22 一、 固相萃取 22 二、 樣品過濾 22 三、 超高效液相層析串聯式質譜儀 22 第四節、 實驗方法 24 一、 樣品前處理 24 二、 以超高效液相層析串聯式質譜儀分析三酸甘油酯 24 三、 以超高效液相層析串聯式質譜儀分析固醇 27 四、 統計分析 28 伍、 結果與討論 29 第一節、 超高效液相層析串聯式質譜儀檢測三酸甘油酯 29 一、 建立逆相液相層析法分離三酸甘油酯 29 二、 質譜儀游離化與三酸甘油酯特徵離子片段 34 三、 偵測極限、定量極限及回收率 41 四、 食用油中三酸甘油酯及脂肪酸分析 42 第二節、 以超高效液相層析儀串聯式質譜儀檢測固醇 66 一、 質譜儀游離化參數條件設定與固醇特徵離子片段 66 二、 檢量線、方法偵測極限與定量極限 66 三、 食用油中自由態固醇含量 69 陸、 結論 73 柒、 參考文獻 74 捌、 附錄 80 一、 三酸甘油酯分析數據品管 80 二、 各食用油中三酸甘油酯之萃取離子層析圖 90 三、 各食用油中三酸甘油酯之組成 100 | |
| dc.language.iso | zh-TW | |
| dc.title | 以超高效液相層析串聯質譜法分析油脂中三酸甘油酯及固醇化合物特徵 | zh_TW |
| dc.title | Characterization of triacylglycerols and sterols in edible oils by ultra-performance liquid chromatography-mass spectrometry | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蘇南維,王惠珠,魏國晉,陳宏彰 | |
| dc.subject.keyword | 三酸甘油酯,食用油,液相層析串聯質譜法,碳當量數,膽固醇,植物固醇, | zh_TW |
| dc.subject.keyword | Edible oil,triacylglycerols,phytosterols,cholesterol,LC-MS,equivalent carbon number, | en |
| dc.relation.page | 140 | |
| dc.identifier.doi | 10.6342/NTU201603440 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-08-22 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-105-1.pdf 目前未授權公開取用 | 8.44 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。