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標題: | 人體頭髮角蛋白與明膠複合靜電紡絲製成之奈米纖維交聯後於周邊神經再生之研發與應用 Crosslinking Of The Electrospun Human Hair Keratin-Gelatin Composite Film For The Promotion Of Peripheral Nerve Regeneration |
作者: | Yen-Yu Chen 陳沿毓 |
指導教授: | 林峰輝 |
關鍵字: | 神經導管,角蛋白, Keratin,Electrospun,Peripheral Nerve Regeneration, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | 對於周圍神經損傷中與神經缺損的部分是現今外科上處理較麻煩的地方。臨床經驗證實,受損的神經段口修復手術,可以使用一個管狀的通道引導,輔助神經修復。從頭髮萃取的角蛋白提供細胞粘附支持並提高細胞的生長為一個適當做為神經導管的材料。在這項研究中,我們致力於開發一個神經導管材料,可以提供更好的生物相容性和機械能力,並在手術過程過後長時間的促進神經再生,然而,角蛋白是不容易單獨形成的靜電纖維。我們用明膠混合角蛋白產生更好的黏度,同時明膠的生物活性和生物相容性也支持神經生長的環境。以甲酸作為溶劑溶解使用的電氣紡絲技術生產角蛋白明膠混合奈米纖維並產生順向的排列,戊二醛作為交聯劑交合角蛋白與明膠混和奈米纖維,希望發現角蛋白比例的增加可能更好地改善材料的強度。分析交聯對化學結構造成變化,細胞培養和生物可降解的生物活性檢測材料膜反應在神經細胞相容性。纖維的形態研究是利用掃描電子顯微鏡和共軛焦顯微鏡技術觀察細胞的突觸延長、排列和成長。
最後,以RT-PCR,觀察許旺細胞的基因表達,證明了角蛋白的加入不但使許旺細胞髓鞘化的表現,更提供了不同的表面特性,於許多生物降解材料已用於製作神經導管修復神經損傷。創建一個新的可生物降解的角蛋白-明膠交聯材料,我們希望研究成果能夠提供周圍神經導管神經損傷發展的貢獻。 The management of peripheral nerve injuries(PNI) with segmental defects is a challenge to surgeon. Clinical experience has shown that damaged nerve can be surgically repaired using a tubular conduit. Keratin from hair has been proposed as an appropriate material that supports cell adherence and improves cell growth. In this study, we focused on the development of artificial nerve guides that could offer better biocompatibility and mechanical ability during surgery and prolonged period of nerve regeneration; however, keratin which is not able to form fiber alone by electrospun. We used gelatin to mix with keratin for better viscosity. With the use of the electrical spinning technique to produce keratin-gelatin mixture fibers in random and parallel direction. Formic acid used as the dissolving solvent. Glutaraldehyde(GTA) as cross-linker to conjugate gelatin with keratin after electrospun. The bioactivity of both gelatin and keratin support a biocompatible environment for nerve. With the Microtensile test we wish to found the increase percentage of keratin may make better improvement of intensity which simply gelatin is poor and easy to hydrolyze. With the FTIR analysis to found what cross-linker make up chemical structure change .The cell culture and biodegradable testing biological activity of keratin-gelatin nanofibers films react to nerve cell. The morphology of the fibers was study by Scanning electron microscopy (SEM) and laser confocal microscopy observations the cells favorably and grow. Finally ,running RT-PCR to observe Schwann cell’s gene expression. Numerous biodegradable materials have been used to make nerve conduit to repair injured nerves. We are now creating a novel biodegradable keratin-gelatin cross-linked conduit which we hope it can facilitate an outcome comparable to autograft in a nerve injury model. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48575 |
全文授權: | 有償授權 |
顯示於系所單位: | 醫學工程學研究所 |
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