請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48575
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林峰輝 | |
dc.contributor.author | Yen-Yu Chen | en |
dc.contributor.author | 陳沿毓 | zh_TW |
dc.date.accessioned | 2021-06-15T07:02:51Z | - |
dc.date.available | 2013-08-23 | |
dc.date.copyright | 2011-08-23 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-19 | |
dc.identifier.citation | 1. Chang, J.-Y., et al., Highly Permeable Genipin-Cross-linked Gelatin Conduits Enhance Peripheral Nerve Regeneration. Artificial Organs, 2009. 33(12): p. 1075-1085.
2. SEDDON, H.J., THREE TYPES OF NERVE INJURY. Brain, 1943. 66(4): p. 237-288. 3. Sunderland, S. and J.W. SMITH, Nerves and Nerve Injuries. Plastic and Reconstructive Surgery, 1969. 44(6): p. 601. 4. Burnett, M.G. and E.L. Zager, Pathophysiology of peripheral nerve injury: a brief review. Neurosurgical FOCUS, 2004. 16(5): p. 1-7. 5. Waters, J., A. Schaefer, and B. Sakmann, Backpropagating action potentials in neurones: measurement, mechanisms and potential functions. Progress in Biophysics and Molecular Biology, 2005. 87(1): p. 145-170. 6. Elizabeth, O.J. and N.S. Panayotis, Nerve repair: Experimental and clinical evaluation of biodegradable artificial nerve guides. Injury, 2008. 39(3): p. 30-36. 7. Aebischer, P., V. Guénard, and R.F. Valentini, The morphology of regenerating peripheral nerves is modulated by the surface microgeometry of polymeric guidance channels. Brain Research, 1990. 531(1-2): p. 211-218. 8. Satoshi, I., I. Yuji, and N. Tatsuo, Artificial nerve tubes and their application for repair of peripheral nerve injury: an update of current concepts. Injury, 2008. 39: p. 29-39. 9. Belkas, J.S., M.S. Shoichet, and R. Midha, Axonal guidance channels in peripheral nerve regeneration. Operative Techniques in Orthopaedics, 2004. 14(3): p. 190-198. 10. Li, J., et al., Electrospinning of Hyaluronic Acid (HA) and HA/Gelatin Blends. Macromolecular Rapid Communications, 2006. 27(2): p. 114-120. 11. Carlisle, C.R., C. Coulais, and M. Guthold, The mechanical stress-strain properties of single electrospun collagen type I nanofibers. Acta Biomaterialia, 2010. 6(8): p. 2997-3003. 12. Detta, N., et al., Novel electrospun polyurethane/gelatin composite meshes for vascular grafts. Journal of Materials Science: Materials in Medicine, 2010. 21(5): p. 1761-1769. 13. Lim, Y.C., et al., Micropatterning and characterization of electrospun poly(ε-caprolactone)/gelatin nanofiber tissue scaffolds by femtosecond laser ablation for tissue engineering applications. Biotechnology and Bioengineering, 2011. 108(1): p. 116-126. 14. Reichl, S., Films based on human hair keratin as substrates for cell culture and tissue engineering. Biomaterials, 2009. 30(36): p. 6854-6866. 15. Hill, P., H. Brantley, and M. Van Dyke, Some properties of keratin biomaterials: Kerateines. Biomaterials, 2010. 31(4): p. 585-593. 16. Rouse, J.G. and M.E. Van Dyke, A Review of Keratin-Based Biomaterials for Biomedical Applications. Materials, 2010. 3(2): p. 999-1014. 17. Hill, P., et al., Repair of peripheral nerve defects in rabbits using keratin hydrogel scaffolds. Tissue Engineering Part A. 0(ja): p. null. 18. Zhang, Y.Z., et al., Crosslinking of the electrospun gelatin nanofibers. Polymer, 2006. 47(8): p. 2911-2917. 19. Alvarez-Perez, M.A., et al., Influence of Gelatin Cues in PCL Electrospun Membranes on Nerve Outgrowth. Biomacromolecules, 2010. 11(9): p. 2238-2246. 20. Kamholz, J., et al., Regulation of Myelin-Specific Gene Expression: Relevance to CMT1. Annals of the New York Academy of Sciences, 1999. 883(1): p. 91-108. 21. Kuczewski, N., et al., Backpropagating Action Potentials Trigger Dendritic Release of BDNF during Spontaneous Network Activity. J. Neurosci., 2008. 28(27): p. 7013-7023. 22. Ludwig, M. and Q.J. Pittman, Talking back: dendritic neurotransmitter release. Trends in Neurosciences, 2003. 26(5): p. 255-261. 23. Lamoureux, P., et al., Growth and elongation within and along the axon. Developmental Neurobiology, 2010. 70(3): p. 135-149. 24. Shao, Y., et al., Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix. In Vitro Cellular & Developmental Biology - Animal, 2010. 46(7): p. 600-605. 25. Ghasemi-Mobarakeh, L., et al., Electrospun poly([var epsilon]-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials, 2008. 29(34): p. 4532-4539. 26. Panseri, S., et al., Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections. BMC Biotechnology, 2008. 8(1): p. 39. 27. Ma, Z., et al., Grafting of Gelatin on Electrospun Poly(caprolactone) Nanofibers to Improve Endothelial Cell Spreading and Proliferation and to Control Cell Orientation. Tissue Engineering, 2005. 11(7-8): p. 1149-1158. 28. Cheng, C.-M., et al., Probing localized neural mechanotransduction through surface-modified elastomeric matrices and electrophysiology. Nat. Protocols, 2010. 5(4): p. 714-724. 29. Nobbio, L., et al., Impairment of PMP22 transgenic Schwann cells differentiation in culture: implications for Charcot-Marie-Tooth type 1A disease. Neurobiology of Disease, 2004. 16(1): p. 263-273. 30. Pereira, J.A., et al., Dicer in Schwann Cells Is Required for Myelination and Axonal Integrity. The Journal of Neuroscience, 2010. 30(19): p. 6763-6775. 31. Quarles, R.H., Myelin-associated glycoprotein (MAG): past, present and beyond. Journal of Neurochemistry, 2007. 100(6): p. 1431-1448. 32. McBeath, R., et al., Cell Shape, Cytoskeletal Tension, and RhoA Regulate Stem Cell Lineage Commitment. Developmental Cell, 2004. 6(4): p. 483-495. 33. Xu, W., et al., Absence of P0 leads to the dysregulation of myelin gene expression and myelin morphogenesis. Journal of Neuroscience Research, 2000. 60(6): p. 714-724. 34. Ratanavaraporn, J., et al., Influences of physical and chemical crosslinking techniques on electrospun type A and B gelatin fiber mats. International Journal of Biological Macromolecules, 2010. 47(4): p. 431-438. 35. Sierpinski, P., et al., The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves. Biomaterials, 2008. 29(1): p. 118-128. 36. Sionkowska, A., et al., Photochemical behaviour of hydrolysed keratin. International Journal of Cosmetic Science, 2011: p. no-no. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48575 | - |
dc.description.abstract | 對於周圍神經損傷中與神經缺損的部分是現今外科上處理較麻煩的地方。臨床經驗證實,受損的神經段口修復手術,可以使用一個管狀的通道引導,輔助神經修復。從頭髮萃取的角蛋白提供細胞粘附支持並提高細胞的生長為一個適當做為神經導管的材料。在這項研究中,我們致力於開發一個神經導管材料,可以提供更好的生物相容性和機械能力,並在手術過程過後長時間的促進神經再生,然而,角蛋白是不容易單獨形成的靜電纖維。我們用明膠混合角蛋白產生更好的黏度,同時明膠的生物活性和生物相容性也支持神經生長的環境。以甲酸作為溶劑溶解使用的電氣紡絲技術生產角蛋白明膠混合奈米纖維並產生順向的排列,戊二醛作為交聯劑交合角蛋白與明膠混和奈米纖維,希望發現角蛋白比例的增加可能更好地改善材料的強度。分析交聯對化學結構造成變化,細胞培養和生物可降解的生物活性檢測材料膜反應在神經細胞相容性。纖維的形態研究是利用掃描電子顯微鏡和共軛焦顯微鏡技術觀察細胞的突觸延長、排列和成長。
最後,以RT-PCR,觀察許旺細胞的基因表達,證明了角蛋白的加入不但使許旺細胞髓鞘化的表現,更提供了不同的表面特性,於許多生物降解材料已用於製作神經導管修復神經損傷。創建一個新的可生物降解的角蛋白-明膠交聯材料,我們希望研究成果能夠提供周圍神經導管神經損傷發展的貢獻。 | zh_TW |
dc.description.abstract | The management of peripheral nerve injuries(PNI) with segmental defects is a challenge to surgeon. Clinical experience has shown that damaged nerve can be surgically repaired using a tubular conduit. Keratin from hair has been proposed as an appropriate material that supports cell adherence and improves cell growth. In this study, we focused on the development of artificial nerve guides that could offer better biocompatibility and mechanical ability during surgery and prolonged period of nerve regeneration; however, keratin which is not able to form fiber alone by electrospun. We used gelatin to mix with keratin for better viscosity. With the use of the electrical spinning technique to produce keratin-gelatin mixture fibers in random and parallel direction. Formic acid used as the dissolving solvent. Glutaraldehyde(GTA) as cross-linker to conjugate gelatin with keratin after electrospun. The bioactivity of both gelatin and keratin support a biocompatible environment for nerve. With the Microtensile test we wish to found the increase percentage of keratin may make better improvement of intensity which simply gelatin is poor and easy to hydrolyze. With the FTIR analysis to found what cross-linker make up chemical structure change .The cell culture and biodegradable testing biological activity of keratin-gelatin nanofibers films react to nerve cell. The morphology of the fibers was study by Scanning electron microscopy (SEM) and laser confocal microscopy observations the cells favorably and grow. Finally ,running RT-PCR to observe Schwann cell’s gene expression.
Numerous biodegradable materials have been used to make nerve conduit to repair injured nerves. We are now creating a novel biodegradable keratin-gelatin cross-linked conduit which we hope it can facilitate an outcome comparable to autograft in a nerve injury model. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T07:02:51Z (GMT). No. of bitstreams: 1 ntu-100-R98548016-1.pdf: 2760313 bytes, checksum: 402c03512672d51ec957e9bb2b50a67d (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 誌謝 I
中文摘要 II ABSTRACT III 1. INTRODUCTION 1 1-1. PERIPHERAL NERVE INJURY 1 1-2. PERIPHERAL NERVE REPAIR 3 1-3. DEVELOPMENT OF NERVE GUIDANCE CONDUIT 5 1-3-1. MATERIALS FOR NERVE GUIDANCE CHANNELS 7 1-3-2. HAIR KERATIN AND GELATIN 10 1-3-3. BIOMOLECULAR SIGNALS 14 1-4. OBJECTIVES OF THIS STUDY 16 2. THEORETICAL BASIS 18 2-1. INTRODUCTION OF NEUROPHYSIOLOGY 18 2-2. THE PERIPHERAL NERVOUS SYSTEM: STRUCTURE AND FUNCTION 18 2-3. PERIPHERAL NERVE INJURY AND REGENERATION 20 2-4. INTRODUCTION OF ELECTROSPINNING TECHNIQUE 24 2-5. GLUTARALDEHYDE AS A CROSSLINKER 26 2-6. SELECTION OF BIOMATERIALS FOR NERVE GUIDANCE CHANNELS 27 2-7. GENE EXPRESSION 27 3. MATERIAL AND METHOD 30 3-1. EXPERIMENTAL APPARATUS 30 3-2. MATERIAL 31 3-3. EXPERIMENTAL FLOW CHART 31 3-4. ELECTROSPINNING SET UP 32 3-5. PREPARATION OF POLYMER SOLUTIONS 33 3-6. CROSSLINK METHODS 35 3-7. FTIR Spectroscopy 36 3-8. Contact Angle Measurements 36 3-9. AFM 37 3-10. DISSOLVABILITY TEST 39 3-11. CELL CULTURE 39 3-12. IN VITRO SCHWANN CELLS PROLIFERATION 40 3-13. LDH LEAKAGE 41 3-14. IMMUNOFLUORESCENCE EXPERIMENTS IN SCHWANN CELL CULTURES 42 3-15. GENE EXPRESSION ANALYSIS BY REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR) 43 3-16. STATISTICAL ANALYSIS 44 4. RESULTS AND DISCUSSION 46 4-1. CROSSLINKED FIBER MORPHOLOGIES BEFORE AND AFTER SOAKING 46 4-2. Contact angle changed by adding keratin 48 4-3. Cytotoxicity 49 4-4. FTIR SPECTROSCOPY 51 4-5. CELL MORPHOLOGY CHANGE 52 4-6. SCHWANN CELL OBSERVATE BY SEM AND IMMUNOFLUORESCENCE OF S100/DAPI 52 4-7. RT-PCR ANALYSIS SHOW OUT 54 5. CONCLUSIONS 57 6. REFERENCE 59 | |
dc.language.iso | en | |
dc.title | 人體頭髮角蛋白與明膠複合靜電紡絲製成之奈米纖維交聯後於周邊神經再生之研發與應用 | zh_TW |
dc.title | Crosslinking Of The Electrospun Human Hair Keratin-Gelatin Composite Film For The Promotion Of Peripheral Nerve Regeneration | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 趙本秀 | |
dc.contributor.oralexamcommittee | 陳悅生,董國忠 | |
dc.subject.keyword | 神經導管,角蛋白, | zh_TW |
dc.subject.keyword | Keratin,Electrospun,Peripheral Nerve Regeneration, | en |
dc.relation.page | 61 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-19 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 2.7 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。