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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48247完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李水盛(Shoei-Sheng Lee) | |
| dc.contributor.author | Wei-Ti Hsu | en |
| dc.contributor.author | 徐偉迪 | zh_TW |
| dc.date.accessioned | 2021-06-15T06:50:05Z | - |
| dc.date.available | 2011-03-03 | |
| dc.date.copyright | 2011-03-03 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-02-18 | |
| dc.identifier.citation | 1. Tomas H., Uwe R., David G., Huyla A., Stefan S., Peter S., Theodore A. M., and
George R. P. J. Org. Chem., 67, 8729-8743 (2002). 2. 台灣藥用植物資源名錄，第一版, 133，行政院衛生署中醫藥委員會，台北市。 3. 蔡振聰,，吳純寬。公園常見花木，第一版，35，淑馨出版社，台北市。 4. 鄭元春。台北市常見的有毒植物，第一版，29，台北市政府新聞處，台北市。 5. Gorter K, Über die vertailung des lycorins in der familie der Amaryllidaceen. Chem Zentr, 3, 846–857 (1920). 6. Pettit G.R., Pettit III G.R., Backhaus R.A., Boyd M.R., Meerow A.W. Antineoplastic agents, 256. Cell growth inhibitory isocarbostyrils from Hymenocallis. J. Nat. Prod., 56, 1682–1687 (1993). 7. Suffnees M, Cordell G.A. Antitumor alkaloids, part XVII. Amaryllidaceae alkaloids. In: Brossi A, ed., The Alkaloids, 25, 198–212 (1985). 8. Martin S.F. The Amaryllidaceae alkaloids. In: Brossi A,ed., The Alkaloids, 30, 351–376 (1987). 9. Mikhail D. A., Nilda T. M., Yolanda R. R. Cytotoxicity of Hymenocallis expansa alkaloids. J. Nat. Prod., 56, 1423-1425 (1993). 10. Long-Ze L., Shu-Fang H., Hee-Byung, C., Thitima P., John M. P., Geoffrey A. Lycorine alkaloids from Hymenocallis littoralis. Phytochemistry, 40, 1295-1298 (1995). 11. Hohmann J., Forgo P., Szabo P. A new phenanthridine alkaloid from Hymenocallis festalis. Fitoterapia, 73, 749-751 (2002). 65 12. Amina H. A.-D., Soad M. T., Hala M. H., Eman S., Eri K., Hiromitsu T. Phytochemistry and bilogical investigation of Hymenocallis littoralis Salisb. Chem. & Biod, 5, 332-340 (2008). 13. George R. P., Stephen A. E., Noeleen M., Brian O., Delbert L. H., Jane M., John C. K., Dennis L. D., George R. P. III, Lynnette C. G., Joy A. B. Isolation and structural modification of 7-deoxynarciclasine and 7-deoxy-trans-dihydronarciclasine. J. Nat. Prod., 69, 7-13 (2006). 14. Nury R., Matilde G., J. D. M. Search for bioactive alkaloids in Hymenocallis species. Pharma. Bio., 42, 280-285 (2004). 15. Folstein M, Folstein S, Mchugh P. “Mini-mental state: a practical method for grading the cognitive state of patients for the clinician. J Psychiatr Res, 12, 189-198 (1975). 16. 郭乃文，劉秀枝，王珮芳等。「簡短式智能評估」之中文施測與常模建立。復 興醫學雜誌。16, 52-59 (1988). 17. Teng E. L., Hasegawa K., Homma A. The Cognitive Abilities Screening Instrument (CASI): A practical test for cross-cultural epidemiological studies of dementia. Int Psychogeriatr, 6, 45-58 (1994). 18. Lin K-N, Wang P-N, Liu C-Y, et al. Cutoff Scores of the Cognitive Abilities Screening Instrument, Chinese Version in Screening of Dementia. Dement Geriatr Cogn Disord, 14, 176-182 (2002) 19. 林克能，劉秀枝。臨床失智評量表。台灣神經學雜誌。12, 154-165 (2003). 20. Bierer, L. M., Haroutunian, V., Gabriel, S., Knott, P. J., Carlin, L. S., Purohit, D. P., Perl, D. P., Schmeidler, J., Kanof, P., and Davis, K. L. Neurochemical correlates of dementia severity in Alzheimer’s disease: Relative importance of the cholinergic deflicits. Journal of Neurochemistry, 64, 740-760 (1995). 66 21. Etienne, P., Dastoor, D., Gauthier, S., Ludwick, R., and Collier, B. Alzheimer’s disease: lack of effect of lecithin treatment for 3 months. Neurology, 31, 1552-1554 (1981). 22. Thal, L. J., Rosen, W., Sharpless, N. S., and Crystal, H. Choline chloride fails to improve cognition of Alzheimer;s disease. Neurobiology of A ging, 2, 205-208 (1981). 23. 劉秀枝，翁文章，王署君。阿茲海默氏症知能之藥物治療。應用心理研究。7, 119-132 (2000). 24. Jurden W. and Huong L. Alkaloids from Hippeastrum equestre herb. -5. Circular dichroism studies. Tetrahedron, 52, 6591-6600 (1996). 25. Lam Huong P., Egon G., Jurgen W., Michael B., Werner D. Two novel amaryllidaceae alkaloids from Hippeastrum equestre herb.: 3-O-demethyltazettine and egonine. Phytochemistry, 51, 327-332 (1999). 26. Diaa T. A. Y. Alkaloids of the flowers of Hippeastrum vittatum. J. Nat. Prod., 64, 839-841 (2001). 27. Francesc V., Jaume B., Gemma T., Carles C., Mario R. Alkaloids from Narcissus bicolor. Phytochemistry, 29, 1307-1310 (1990). 28. Steven W., Baldwin and John S. Debenham. Total syntheses of (-)-Haemanthidine, (+)-Pretazettine, and (+)-Tazettine. Organic Letters, 2, 99-102 (2000). 29. Flieger M., Kren V., Zelenkova N. F. Ergot alkaloids glycosides from saprophytic cultures of claviceps, II. Chanoclavine I fructosides. Journal of Natural Products, 53, 171-175 (1990). 30. Pistia-Brueggeman G. and Hollingsworth R. A preparation and screening strategy for glycosidase inhibitors. Tetrahedron, 57, 8773-8778 (2001). 31. 曾建誠，台灣大學醫學院藥學研究所碩士論文，山欖葉部之成分研究. 2008, Nov. 67 32. Asano N. Glycosidase inhibitors: update and perspectives on practical use. Glycobiology, 13, 93-104 (2003). 33. Krentz A. J. and Bailey C. J. Oral antidiabetic agents-Current role in type 2 diabetes mellitus. Drugs, 65, 385-411 (2005). | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48247 | - |
| dc.description.abstract | 在文獻中，得知石蒜科植物富含生物鹼。在這些生物鹼當中，雪花胺(galanthamine)具有抗乙醯膽鹼分解酶的活性，而且已於臨床上用以減緩阿茲海默症的症狀。這些論點則驅使我們致力於研究石蒜科植物，螯蟹花中具有抗乙醯膽鹼分解酶的生物鹼。
從螯蟹花的葉部中，經由基本的酸鹼處理和液相-液相極性分割萃取出生物鹼。這部分具有抗乙醯膽鹼分解酶的活性，之後再藉由離心式分配層析、葡萄糖膠管柱層析、矽膠管柱層析、高效液相層析方法、液相層析-固相萃取-核磁共振儀及液相層析-高解析電灑法質譜作進一步的分離。這些成果已分離出tazettine, ismine, bicolorine和pretazettine這些生物鹼。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-15T06:50:05Z (GMT). No. of bitstreams: 1 ntu-100-R96423014-1.pdf: 6243178 bytes, checksum: b3dd9dc00022ccbef0360a2beccbad2d (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 總目錄
口試委員會審定書……………………………………………………………………i 中文摘要.........................................................................................................................ii 英文摘要........................................................................................................................iii 目錄................................................................................................................................I 表目錄..........................................................................................................................III 圖目錄..........................................................................................................................III 附圖目錄.......................................................................................................................V 流程圖目錄................................................................................................................VII 詞彙 (Glossary)………………………………………………………………… ..VIII 目錄 壹、序論 ......................................................................................................................... 1.1 研究目的 ……………………………………………………………………..1 1.2 植物簡介 ……………………………………………………………………..2 1.3 Hymenocallis 屬植物成分之相關研究...........................................................4 1.4 阿茲海默症及臨床治療藥物 ………………………………………………8 1.4.1 阿茲海默症簡介 ……………………………………………………8 1.4.2 阿茲海默症治療藥物 ………………………………………………8 1.5 LC-SPE-NMR Hyphenation 之簡介 10 貳、實驗結果與討論 ..................................................................................................... 2.1 螯蟹花葉部成分之乙醇萃取物與活性測試結果 ………………………11 2.2 生物鹼成分之分離及結構解析…...………………………………………13 2.2.1 Tazettine (1)之結構解析……………………………………………..14 2.2.2 Ismine (2)之結構解析………………………………………………..18 2.2.3 Bicolorine (3)之結構解析.................................................................. .22 2.2.4 Pretazettine (4)之結構解析……………………….…………….……27 2.3 利用LC-HRESIMS，另再以1H、13C-NMR 及DEPT 135 & 90 與DNP (Dictionary of Natural Products)於混合物中比對化合物 ……………………30 2.3.1 Fr. 1-2 之圖譜及相關資料…………………………………………...31 2.3.2 Fr. 6之圖譜及相關資料………………………………………...........33 2.3.3 Fr. 9之圖譜及相關資料……………………………………………...35 2.3.4 Fr. 10之圖譜及相關資料………………………………………….....37 2.3.5 Fr. 11之圖譜及相關資料………………………………………….....38 2.3.6 Fr. 12之圖譜及相關資料………………………………..…………...40 2.3.7 Fr. 13之圖譜及相關資料………………………………..…………...41 2.4 討論 …………………………………………………………………………43 2.4.1化合物3之結構解析與文獻比較……………………………………43 2.4.2 Ismine與Bicolorine之可能生合成路徑………………………….....44 2.4.3 利用LC-HRESIMS、1H及13C-NMR、與DNP (Dictionary of Natural Products)於混合物中比對化合物之探討……………………………..…..44 2.5 結論……………………….............................................................................45 參、實驗部分 ................................................................................................................. 3.1 儀器與材料……………………………………………………………….....46 3.1.1 理化性質測定儀器………………………………………………...46 3.1.2 成分分離之儀器及材料……………………………………………47 3.1.3 試劑與溶媒…………………………………………………………..48 3.1.4甲型葡萄糖水解酶活性抑制試驗 (-glucosidase inhibition assay) 所 用試劑與儀器……………………………………………………………..49 3.1.5 乙醯膽鹼分解酶之活性抑制試驗 (acetylcholinesterase inhibition assay) 所用試劑與儀器……………………………………………………49 3.2 抑制甲型葡萄糖水解酶之活性試驗 (-glucosidase inhibition assay).....50 3.2.1 原理………………………………………………………………….50 3.2.2 酵素活性單位……………………………………………………….51 3.2.3 實驗方法…………………………………………………………….51 3.3 乙醯膽鹼分解酶之活性抑制試驗 (acetylcholinesterase inhibition assay) 54 3.3.1 原理………………………………………………………………...54 3.3.2 實驗方法……………………………………………………………..54 3.4 螯蟹花葉部成分之萃取與分離……………………………………………57 3.4.1 Tazettine (1)之分離…………………………………………………60 3.4.2 Ismine (2)之分離……………………………………………………..60 3.4.3 Bicolorine (3)之分離………………………………………………...61 3.4.4 Pretazettine (4)之分離……………………………………………….61 3.5 化合物1 到4 之物理數據…………………………………………………62 3.5.1 Tazettine (1)…………………………………………………………..62 3.5.2 Ismine(2)……………………………………………………………...62 3.5.3 Bicolorine (3)………………………………………………………...63 3.5.4 Pretazettine (4)……………………………………………………….63 參考文獻……………..………………………………………………………………..64 | |
| dc.language.iso | zh-TW | |
| dc.subject | 水鬼蕉屬 | zh_TW |
| dc.subject | 螯蟹花 | zh_TW |
| dc.subject | 石蒜科 | zh_TW |
| dc.subject | Hymenocallis | en |
| dc.subject | Amaryllidaceae | en |
| dc.subject | Hymenocallis Speciosa | en |
| dc.title | 螯蟹花葉部之成分研究 | zh_TW |
| dc.title | Chemical investigation of the leaves of
Hymenocallis Speciosa ( L.f. ex Salisb) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳春雄,郭錦樺 | |
| dc.subject.keyword | 螯蟹花,石蒜科,水鬼蕉屬, | zh_TW |
| dc.subject.keyword | Hymenocallis Speciosa,Amaryllidaceae,Hymenocallis, | en |
| dc.relation.page | 122 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-02-18 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 藥學研究所 | zh_TW |
| 顯示於系所單位： | 藥學系 | |
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