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標題: | 在玻璃基材上利用聚乙烯醇和CO2雷射快速製造具排列性微組織應用於皮膚與毛囊再生 Rapid laser-assisted patterning of three heterotypic cell types on poly (vinyl alcohol) coated substrate for skin and hair follicle regeneration |
作者: | Meng-Wei Lin 林孟薇 |
指導教授: | 楊台鴻 |
關鍵字: | 皮膚再生,毛囊再生,雷射,聚乙烯醇,細胞排列, skin regeneration,hair regeneration,laser,PVA,cell patterning, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | 人工皮膚從80年代發展至今,已成功誘導皮膚再生,減少傷疤的形成。所形成之皮膚具有觸覺也能感受冷熱變化,但缺少毛囊與汗腺等腺體,對於頭皮受傷之患者在進行皮膚修復的過程中還需進行繁複的毛囊移植手術。
目前在毛囊再生研究中,已知真皮乳突細胞具有誘導角質細胞形成毛囊的能力,而真皮乳突細胞團的誘導能力更好;且已有許多方法在體外先形成真皮乳突細胞團或是真皮乳突細胞和角質細胞混合的細胞團,但在實際應用上尚需單一植入且生成之毛髮分布不均,在移植效率上仍屬冗長。 本研究結合了雷射製程,能夠快速製造出所需的間距和孔洞大小,配合poly(vinyl alcohol) (PVA),模擬皮膚組織之排列,成功製造出直徑約200μm的真皮乳突細胞團,以300μm之間距矩陣排列於真皮纖維母細胞間,其上有角質細胞覆蓋之細胞晶片。 以此晶片可以針對細胞間的交互作用做分析,如分泌之細胞間質(ECM)或生長因子(GF)的調控;未來也可將此細胞晶片利用適當方法取下做為移植之用,也可應用於兩種間質細胞的排列。 Artificial skin has developed over twenty years, it could regenerate dermis and avoid scar formation. The neodermis could sense touch, cold and hot, but lack of appendages, like hair follicle and sudoriferous glands, and it is comlex to operate surgery on the scalp. From previous studies, we know dermal papillae could induce hair follicle regeneration, especially the aggregation form. It has developed many models to produce dermal papillae microtissues in vitro, but the efficiency and orientation are still low. Our study uses CO2 laser to rapidly fabricate different patterns and confine dermal papillae to aggregate into the holes by poly(vinyl alcohol). Then pattern fibroblasts and keratinocytes with the time-window of PVA barrier and the adhesive ability of cells. The resultant model shows that only the microtissues in the hole have strong ability to induce hair follicles. The model could apply in the research of interactions between more cells and the pattern of two mesenchymal cells. Furthermore, it could be a stage for future cell sheet implantation. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48134 |
全文授權: | 有償授權 |
顯示於系所單位: | 醫學工程學研究所 |
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ntu-100-1.pdf 目前未授權公開取用 | 20.39 MB | Adobe PDF |
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