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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻 | |
dc.contributor.author | Meng-Wei Lin | en |
dc.contributor.author | 林孟薇 | zh_TW |
dc.date.accessioned | 2021-06-15T06:47:02Z | - |
dc.date.available | 2016-07-07 | |
dc.date.copyright | 2011-07-07 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-06-14 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48134 | - |
dc.description.abstract | 人工皮膚從80年代發展至今,已成功誘導皮膚再生,減少傷疤的形成。所形成之皮膚具有觸覺也能感受冷熱變化,但缺少毛囊與汗腺等腺體,對於頭皮受傷之患者在進行皮膚修復的過程中還需進行繁複的毛囊移植手術。
目前在毛囊再生研究中,已知真皮乳突細胞具有誘導角質細胞形成毛囊的能力,而真皮乳突細胞團的誘導能力更好;且已有許多方法在體外先形成真皮乳突細胞團或是真皮乳突細胞和角質細胞混合的細胞團,但在實際應用上尚需單一植入且生成之毛髮分布不均,在移植效率上仍屬冗長。 本研究結合了雷射製程,能夠快速製造出所需的間距和孔洞大小,配合poly(vinyl alcohol) (PVA),模擬皮膚組織之排列,成功製造出直徑約200μm的真皮乳突細胞團,以300μm之間距矩陣排列於真皮纖維母細胞間,其上有角質細胞覆蓋之細胞晶片。 以此晶片可以針對細胞間的交互作用做分析,如分泌之細胞間質(ECM)或生長因子(GF)的調控;未來也可將此細胞晶片利用適當方法取下做為移植之用,也可應用於兩種間質細胞的排列。 | zh_TW |
dc.description.abstract | Artificial skin has developed over twenty years, it could regenerate dermis and avoid scar formation. The neodermis could sense touch, cold and hot, but lack of appendages, like hair follicle and sudoriferous glands, and it is comlex to operate surgery on the scalp. From previous studies, we know dermal papillae could induce hair follicle regeneration, especially the aggregation form. It has developed many models to produce dermal papillae microtissues in vitro, but the efficiency and orientation are still low.
Our study uses CO2 laser to rapidly fabricate different patterns and confine dermal papillae to aggregate into the holes by poly(vinyl alcohol). Then pattern fibroblasts and keratinocytes with the time-window of PVA barrier and the adhesive ability of cells. The resultant model shows that only the microtissues in the hole have strong ability to induce hair follicles. The model could apply in the research of interactions between more cells and the pattern of two mesenchymal cells. Furthermore, it could be a stage for future cell sheet implantation. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T06:47:02Z (GMT). No. of bitstreams: 1 ntu-100-R98548014-1.pdf: 20875579 bytes, checksum: 44cba38b3cdf2ad2bdf945ea6dbc25b5 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 致謝 I
ABSTRACT III 摘 要 IV 目 錄 V 圖表目錄 VIII 第一章、 序論 1 1.1. 皮膚的損傷和治療 1 1.2. 人工皮膚 3 1.3. 毛囊構造與發育 7 1.4. 毛囊再生在體外的研究 11 1.5. 高分子材料於生醫上的應用 13 1.6. 微製程在組織工程上的應用 14 第二章、 實驗目的與設計 15 2.1. 實驗目的 15 2.2. 實驗設計 18 第三章、 材料與方法 20 3.1. 真皮乳突細胞之培養 20 3.2. 角質細胞之分離 20 3.3. 真皮纖維母細胞之培養 21 3.4. 聚乙烯醇薄膜的製備 21 3.5. CO2快速雷射成圖 22 3.6. 細胞密度 22 3.7. 孔洞大小和間距 23 3.8. 紅外線光譜儀 (FTIR)檢測聚乙烯醇 23 3.9. 真皮乳突細胞團之個數和存活率 23 3.10. 真皮乳突細胞、真皮纖維母細胞和角質細胞於TCPS、聚乙烯醇和玻璃的貼附度 24 3.11. 真皮纖維母細胞於聚乙烯醇/玻璃晶片上隨時間之貼附度 24 3.12. 角質細胞於真皮纖維母細胞與真皮乳突細胞之貼附度 24 3.13. 掃描式電子顯微鏡 (SEM) 之觀察 25 3.14. 共軛焦顯微鏡之觀察 25 3.15. 免疫染色 26 3.16. 鹼性磷酸酶染色 26 3.17. 曠時攝影 26 3.18. RNA萃取 27 3.19. 反轉錄聚合酶鏈鎖反應 27 3.20. 即時聚合酶鏈鎖反應 28 3.21. 蛋白質隨時間的吸附 29 第四章、 實驗結果 30 4.1. 以聚乙烯醇塗佈之玻璃表面 30 4.2. 真皮乳突細胞、真皮纖維母細胞和角質細胞在TCPS、PVA和玻璃上之貼附度 32 4.3. 細胞密度對排列性立體微組織的影響 36 4.4. PVA/玻璃晶片之大小和間距 38 4.5. 真皮乳突細胞團之數量與存活率 44 4.6. 角質細胞、真皮乳突細胞與真皮纖維母細胞的作用力 47 4.7. 真皮纖維母細胞於晶片上貼附之時間效應 49 4.8. 不同的細胞培養順序之影響 52 4.9. PVA/玻璃晶片之動態分析 57 4.10. 在PVA/玻璃晶片上之細胞形態 59 4.11. 細胞活性與基因表現 66 第五章、 討論 71 第六章、 結論 76 第七章、 參考文獻 77 | |
dc.language.iso | zh-TW | |
dc.title | 在玻璃基材上利用聚乙烯醇和CO2雷射快速製造具排列性微組織應用於皮膚與毛囊再生 | zh_TW |
dc.title | Rapid laser-assisted patterning of three heterotypic cell types on poly (vinyl alcohol) coated substrate for skin and hair follicle regeneration | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳君泰,林致廷 | |
dc.subject.keyword | 皮膚再生,毛囊再生,雷射,聚乙烯醇,細胞排列, | zh_TW |
dc.subject.keyword | skin regeneration,hair regeneration,laser,PVA,cell patterning, | en |
dc.relation.page | 83 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-06-15 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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