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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林璧鳳 | |
dc.contributor.author | Yi-Chi Chen | en |
dc.contributor.author | 陳奕綺 | zh_TW |
dc.date.accessioned | 2021-06-15T06:17:45Z | - |
dc.date.available | 2015-08-20 | |
dc.date.copyright | 2010-08-20 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-10 | |
dc.identifier.citation | 苗明三 (1997) 實驗動物和實驗動物技術p127-132 中國人民出版社
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47773 | - |
dc.description.abstract | 本研究主要探討含Lactobacillus casei Shirota (LcS)之發酵乳製品,對氣喘模式小鼠呼吸道發炎與過敏免疫反應之影響。首先以致敏小鼠探討含LcS發酵乳製品對過敏性氣喘的影響。BALB/c雌鼠八週大時,以卵蛋白(ovalbumin, OVA)與氫氧化鋁為佐劑進行腹腔致敏,以誘發過敏體質。第四次致敏後隔週以血清中OVA特異性IgE進行分組,分別為控制組(OVA/H2O)、不含LcS的乳製品為安慰劑組(OVA/P)、含LcS發酵乳製品低(OVA/L)、中(OVA/M)和高(OVA/H)劑量組與prednisolone藥物組(OVA/Pred),另有一組為未致敏小鼠(PBS/H2O),共7組。分組後即開始餵食樣品,於餵食期間以5%霧化之OVA給予致敏小鼠呼吸道刺激,誘發過敏性呼吸道發炎。餵食11週後,以methacholine (Mch)刺激呼吸道,測定小鼠呼吸道過度反應(airway hyperresponsiveness, AHR)。餵食13週後犧牲小鼠,採其血液、脾臟、肺氣管沖洗液、腸繫膜淋巴結細胞(mesenteric lymph node, MLN)與小腸沖洗液。結果顯示,LcS各劑量組於50 mg/mL Mch刺激下,呼吸道阻力係數Penh值皆顯著低於OVA/H2O組,顯示LcS可降低呼吸道平滑肌過度反應。在肺沖洗液中,LcS各劑量組皆顯著降低IL-6含量,隨著LcS劑量增加,IL-13含量於OVA/H組達到顯著降低。OVA/M組與OVA/H組小鼠脾臟細胞IL-2分泌量顯著降低,OVA/H組IL-4分泌量同時顯著降低,MLN細胞IL-2分泌量在OVA/M組亦顯著降低,在OVA/H組IL-4分泌量達到顯著降低,故OVA/M組血清中OVA特異性IgE抗體趨勢較低且顯著減少總IgE,OVA/H組兩者皆顯著降低,OVA/H組腸沖洗液sIgA含量顯著增加。根據本研究結果,LcS具有劑量效應,高劑量下可透過腸道免疫降低氣喘小鼠IL-4的分泌、減少過敏性抗體IgE的生成並減少促發炎介質的分泌,有助於減緩過敏性氣喘的症狀。接著以heat-killed LcS凍乾粉末培養BALB/c小鼠骨髓衍生之樹突細胞,發現heat-killed LcS可以顯著增加樹突細胞IL-10和IL-12的分泌,可能影響naïve T 細胞傾向分化成Th1或Treg細胞,因此具有改善傾向Th2的過敏免疫反應。 | zh_TW |
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dc.description.tableofcontents | 目錄 I
表目錄 V 圖目錄 VI 中文摘要 VII 英文摘要 VIII 縮寫對照表 X 第一章、序論 第一節 文獻回顧 1 一、 過敏性氣喘 1 (一) 過敏性氣喘之簡介 1 (二) 輔助型T細胞與調節型T細胞對過敏反應的影響 2 1. Th1/Th2平衡 2 2. 調節型T細胞 2 (三) 衛生假說 3 (四) 過敏性氣喘發生機制 4 二、 黏膜免疫系統 5 (一) 黏膜免疫之簡介 5 (二) 腸道淋巴免疫系統 6 三、 益生菌 7 (一) 益生菌簡介 7 (二) 益生菌的免疫調節物質 8 1. CpG-oligodeoxynucleotides 8 2. Lipopolysaccharides 9 3. 其他 9 (三) 乳酸菌 10 第二節 研究動機 13 第二章、LACTOBACILLUS CASEI SHIROTA對氣喘模式小鼠腸道與過敏免疫反應的影響 第一節 前言 14 第二節 材料與方法 15 一、 樣品來源 15 二、 動物實驗設計 15 (一) 動物飼養 15 (二) 致敏方式 16 (三) 劑量換算 16 (四) 分組與實驗流程 18 (五) 呼吸道過度反應測定 19 三、動物犧牲及實驗材料收集與分析 20 (一) 血液樣品的收集 20 (二) 血清特異性抗體IgE、IgG1、IgG2a測定 20 (三) 血清總IgE、IgG、IgA、IgM抗體含量測定 21 (四) 肺氣管沖洗液細胞之取得與處理 22 (五) 脾臟細胞之取得與培養 23 (六) 脾臟細胞增生測定 24 (七) 腸繫膜淋巴結細胞之取得與培養 25 (八) 皮耶氏體細胞之取得與培養 25 (九) 小腸沖洗液之取得 26 (十) 細胞激素含量測定 26 (十一) 吞噬細胞活性測定 29 (十二) 自然殺手細胞活性分析 30 四、統計方法 31 第三節 結果 32 一、 生長情形與攝食狀況 32 二、 器官重量與相對重量 32 三、 呼吸道過度反應 32 四、 肺氣管沖洗液各項指標變化 33 五、 肺氣管沖洗液中各種類細胞數目 33 六、 脾臟細胞增生反應 33 七、 脾臟細胞細胞激素分泌量 33 八、 腸繫膜淋巴結之細胞激素分泌量 34 九、 皮耶氏體細胞之細胞激素分泌量 35 十、 小腸沖洗液中secretory IgA含量 35 十一、血清中OVA特異性IgE、IgG1與IgG2a 抗體含量 35 十二、血清中總IgE、IgM、IgG與IgA抗體含量 35 十三、循環血液中吞噬細胞之吞噬能力 36 十四、脾臟中自然殺手細胞活性 36 第四節 討論 54 一、 餵食LcS之小鼠生長情形 54 二、 餵食LcS對過敏性氣喘小鼠呼吸道發炎之影響 54 三、 餵食LcS對過敏性小鼠全身性免疫反應之影響 56 四、 餵食LcS對GALT之影響 56 五、 餵食LcS對過敏性小鼠血清中抗體生成之影響 58 第三章、LACTOBACILLUS CASEI SHIROTA對骨髓衍生之樹突細胞的影響 第一節 前言 59 第二節 材料與方法 60 一、 Heat-killed L. casei Shirota凍乾粉末製備 60 二、 BALB/c小鼠骨髓衍生樹突細胞之取得與培養 60 三、 細胞激素含量測定 62 四、 統計方法 62 第三節 結果 63 一、 Heat-killed L. casei Shirota對骨髓衍生之樹突細胞存活率的影響 63 二、 Heat-killed L. casei Shirota對骨髓衍生之樹突細胞IL-12與IL-10的影 響 63 第四節 討論 66 第四章、綜合討論與總結 第一節 綜合討論 69 一、 OVA致敏小鼠模式 69 二、 Prednisolone對小鼠免疫反應的影響 70 三、 LcS具有劑量效應 70 四、 LcS影響腸道免疫反應可能機制 71 第二節 總結 74 第五章 參考文獻 75 | |
dc.language.iso | zh-TW | |
dc.title | Lactobacillus casei Shirota對氣喘模式小鼠
腸道與過敏免疫反應的影響 | zh_TW |
dc.title | Effects of Lactobacillus casei Shirota on gut mucosal and allergic immune responses in a murine model of asthma | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 江伯倫,江文章,蔡英傑,吳文勉 | |
dc.subject.keyword | Lactobacillus casei Shirota,卵蛋白,氣喘,呼吸道過度反應,IgE, | zh_TW |
dc.subject.keyword | Lactobacillus casei Shirota,ovalbumin,asthma,airway hyperresponsiveness,IgE, | en |
dc.relation.page | 82 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-11 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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