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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47204| Title: | 產製人類肺表面張力蛋白質 B/C 基因轉殖小鼠之研究 Generation of Transgenic Mice Harboring Human Pulmonary Surfactant-related Protein B/C Genes |
| Authors: | Yun-Yi Ma 馬耘逸 |
| Advisor: | 吳信志(Shinn-Chih Wu) |
| Keyword: | 雙基因共同注射,基因轉殖小鼠,B 型肺臟表面張力蛋白質,C型蛋白質,乳汁, microinjection,transgenic mice,surfactant protein B,surfactant protein C,milk, |
| Publication Year : | 2010 |
| Degree: | 碩士 |
| Abstract: | 3
摘要 本研究主旨在探討小鼠原核胚經雙基因共同注射後，於體外培養至二細 胞時期再進行胚移置對產製攜帶雙基因轉殖小鼠效率之影響。轉殖基因之構築 係以乳腺專一表現之牛 74 英文摘要 Generation of Transgenic Mice Harboring Human Pulmonary Surfactant-related Protein B/C Genes Yun-Yi Ma Abstract The purpose of this study was to investigate the effect of advanced culture to two-cell stage prior to co-injected mouse eggs on pups born and transgene integration efficiency by pronuclear gene microinjection. Transgenes were constructed by using a mature surfactant protein B (mSP-B) and C (mSP-C) cDNA fragment equipped with bovine |
| URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47204 |
| Fulltext Rights: | 有償授權 |
| Appears in Collections: | 動物科學技術學系 |
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| File | Size | Format | |
|---|---|---|---|
| ntu-99-1.pdf Restricted Access | 4.19 MB | Adobe PDF |
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