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  1. NTU Theses and Dissertations Repository
  2. 生物資源暨農學院
  3. 食品科技研究所
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45740
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DC 欄位值語言
dc.contributor.advisor周正俊(Cheng-Chun Chou)
dc.contributor.authorMeng-Hsuan Linen
dc.contributor.author林孟璇zh_TW
dc.date.accessioned2021-06-15T04:45:22Z-
dc.date.available2015-08-19
dc.date.copyright2010-08-19
dc.date.issued2010
dc.date.submitted2010-08-05
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dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45740-
dc.description.abstract本研究乃試圖了解不同食源性病原菌包括Vibrio parahaemolyticus、Salmonella Typhimurium及Listeria monocytogenes經過熱震與冷震之次致死壓力處理後,於後續暴露在氯消毒劑Clidox-S® 及四級銨消毒劑Quatricide® 中之存活情形。
結果顯示,熱震處理可顯著地 (p < 0.05) 提升V. parahaemolyticus與L. monocytogenes對Clidox-S&reg; 及Quatricide&reg; 之耐受性,然而卻降低了Sal. Typhimurium對這些消毒劑之耐受性。在另一方面經過冷震處理後之V. parahaemolyticus、Sal. Typhimurium及L. monocytogenes菌體暴露於Clidox-S&reg;及Quatricide&reg;之耐受性亦顯著提高 (p < 0.05),而在40oC下,Clidox-S&reg; 及Quatricide&reg; 對所試病原菌呈現之抗菌效果均比在25oC下強。
zh_TW
dc.description.abstractThis study was conducted to investigate the effct of heat shock and cold shock on the susceptibility of various food-borne pathogens such as Vibrio parahaemolyticus, Salmonella Typhimurium and Listera monocytogenes to sanitizers including Clidox-S&reg; and Quatricide&reg;.
It was found that heat shock treatment significantly (p < 0.05) increased the tolerance of V. parahaemolyticus and L. monocytogenes to Clidox-S&reg; and Quatricide&reg;, while reduced the tolerance of Sal. Typhimurium to these sanitizers. On the other hand, a significantly (p < 0.05) increased tolerance to Clidox-S&reg; and Quatricide&reg; was noted with cells of V. parahaemolyticus, Sal. Typhimurium and L. monocytogenes after exposure to cold shock treatment. Additionally, Clidox-S&reg; and Quatricide&reg; exhibited a higher antibacterial activity against the pathogens examined at 40oC that at 25oC.
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dc.description.tableofcontents中文摘要 i
Abstract ii
目錄 iii
表目錄 vi
圖目錄 vii
壹、 前言 1
貳、 文獻整理 2
一、 食源性病原菌 2
(一) V. parahaemolyticus 2
1. 基本特性 2
2. 分布及來源 3
3. V. parahaemolyticus之食品中毒與致病性 3
(二) Salmonella 5
1. 基本特性 5
2. 分布及來源 6
3. Salmonella之食品中毒與致病性 6
(三) L. monocytogenes 7
1. 基本特性 7
2. 分布及來源 8
3. L. monocytogenes之食品中毒與致病性 8
二、 壓力反應 9
(一) 熱震反應 (Heat shock response) 10
(二) 冷震反應 (Cold shock response) 11
三、 消毒劑 12
(一) 氯化合物 14
(二) 四級銨化合物 16
參、 材料與方法 18
一、 實驗材料 18
(一) 菌種 18
(二) 培養基 18
(三) 試驗藥品 18
(四) 儀器設備 18
二、 實驗方法 18
(一) 菌株之保存 19
(二) 實驗菌株接種源之製備 19
(三) 熱震處理 19
1. V. parahaemolyticus 19
2. Sal. Typhimurium 19
3. L. monocytogenes 20
(四) 冷震處理 20
1. V. parahaemolyticus 20
2. Sal. Typhimurium 20
3. L. monocytogenes 20
(五) 次致死壓力影響不同食源性病原菌於消毒劑中之存活試驗 21
1. 消毒劑之製備 21
2. 耐受性試驗之進行 21
(六) 菌數測定 21
(七) 統計分析 22
肆、 結果與討論 23
一、 熱震處理之影響 23
(一) 熱震處理影響不同食源性病原菌對於消毒劑Clidox-S&reg;之敏感性 23
(二) 熱震處理影響不同食源性病原菌對於消毒劑Quatricide&reg;之敏感性 28
二、 冷震處理之影響 34
(一) 冷震處理影響不同食源性病原菌對於消毒劑Clidox-S&reg;之敏感性 34
(二) 冷震處理影響不同食源性病原菌對於消毒劑Quatricide&reg;之敏感性 38
伍、 結論 42
陸、 參考文獻 43
柒、 附錄 52
dc.language.isozh-TW
dc.subject消毒劑zh_TW
dc.subjectVibrio parahaemolyticuszh_TW
dc.subjectSalmonella Typhimuriumzh_TW
dc.subjectListeria monocytogeneszh_TW
dc.subject熱震zh_TW
dc.subject冷震zh_TW
dc.subjectSalmonella Typhimuriumen
dc.subjectCold shocken
dc.subjectHeat shocken
dc.subjectListeria monocytogenesen
dc.subjectVibrio parahaemolyticusen
dc.subjectSanitizersen
dc.title熱震與冷震影響一些食源性病原菌對消毒劑之敏感性zh_TW
dc.titleHeat and Cold shock Affect the Susceptibility of Various Food-borne Pathogens to Sanitizersen
dc.typeThesis
dc.date.schoolyear98-2
dc.description.degree碩士
dc.contributor.oralexamcommittee丘志威,潘崇良,蔡國珍,游若?
dc.subject.keywordVibrio parahaemolyticus,Salmonella Typhimurium,Listeria monocytogenes,熱震,冷震,消毒劑,zh_TW
dc.subject.keywordVibrio parahaemolyticus,Salmonella Typhimurium,Listeria monocytogenes,Heat shock,Cold shock,Sanitizers,en
dc.relation.page64
dc.rights.note有償授權
dc.date.accepted2010-08-06
dc.contributor.author-college生物資源暨農學院zh_TW
dc.contributor.author-dept食品科技研究所zh_TW
顯示於系所單位:食品科技研究所

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