以Gluconacetobacter xylinus連續式生產細菌纖維素薄膜之研究

Abstract

許多文獻探討如何利用氣舉裝置或攪拌槽的反應器生產細菌纖維素。這個研究是要探討如何以連續式的操作模式來生產纖維素薄膜。 本實驗透過饋料式和連續式的操作來觀察反應器中各項數據變化並同時以批次操作的數據來互相比較。在本實驗中發現，利用兩纖維素薄膜互相連接的特性來減少反應器空間利用的問題。

本實驗於批次操作第四天時開始連續式操作，每三天添加改良後(pH8.0葡萄糖濃度50g/L) 40ml的BSH培養基，並將生產的纖維素薄膜以相隔反應器內培養基0~1mm左右的距離置放，添加八次相同條件培養基後(二十四天)可以得到長度將近170公分且乾重將近2.3g的細菌纖維素。在這其中，每三天大約都保持類似程度的纖維素合成量和長度。

本反應器可以生產任意長度的細菌纖維素薄膜。並提供另一種生產纖維素的模式，同時也看到降低生產纖維素成本的可能性。

Many studies have been conducted to produce bacterial cellulose in the agitated reactors. This research aimed to continuously produce bacterial cellulose pellicles. The experiment was conducted in fed-batch and continuous operation and the results were compared to that in batch operation. The connection of two BC pellicles was found to design the continuous operation of the reactor. The continuous operation started at the chosen time of the batch operation adding 40ml modified BSH (0.5% yeast extract, 0.5% peptone, 0.115% citric acid, 0.27% Na2HPO4•12H2O and 5% D-glucose pH 8.0) medium every three days produced 170cm, 2.3g bacterial cellulose pellicle(dry basis) after twenty-four days. Before the formed BC pellicle connected to the pellicle synthesized in the following three days, the maximum distance between the formed pellicle and the medium was set at 1mm. The reactor can not only provide a alternative way to produce bacterial cellulose, but also produce arbitrary length of bacterial cellulose pellicle. Also, the reactor may lower the price of bacterial cellulose which can make the price of bacterial cellulose more competitive.