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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李篤中(Duu-Jong Lee) | |
dc.contributor.author | Shun-Chieh Lee | en |
dc.contributor.author | 李舜傑 | zh_TW |
dc.date.accessioned | 2021-05-14T17:42:21Z | - |
dc.date.available | 2017-02-01 | |
dc.date.available | 2021-05-14T17:42:21Z | - |
dc.date.copyright | 2016-02-24 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2016-01-04 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/4453 | - |
dc.description.abstract | 本研究合成並純化有機高分子材料「聚丙二醇-二腺嘌呤」,並以核磁共振、質譜儀與凝膠滲透層析法確立其結構。掃描式與穿透式電子顯微鏡,以及動態雷射光散射技術顯示聚丙二醇-二腺嘌呤溶於水中會形成奈米顆粒,其平均粒徑約為100奈米。聚丙二醇-二腺嘌呤的臨界微胞濃度(CMC)以芘(pyrene)的螢光光譜決定,其結果為8×10^(-2) mg/mL。最低臨界溶解溫度(LCST)係以紫外光�可見光光譜儀測定,結果發現聚丙二醇-二腺嘌呤的最低臨界溶解溫度會隨其在水中濃度變化,且其在濃度越高時變化幅度越小。此材料最低臨界溶解溫度範圍涵蓋人體體溫。初步的藥物載入與釋放實驗顯示,此材料有很好的藥物包覆率與最低臨界溶解溫度附近快速的藥物釋放表現,有潛力作為標靶藥物傳輸之載體。 | zh_TW |
dc.description.abstract | The polymer PPG-bis-adenine was synthesized and purified, and the chemical structure was characterized by NMR, mass spectroscopy, and chromatography. Scanning electron microscopy, transmission electron microscopy and dynamic light scattering showed the existence of nanoparticles formed by PPG-bis-adenine, and the mean diameter of nanoparticles was determined to be about 100 nm in aqueous solution. The critical aggregation concentration was determined using pyrene fluorescent emission and was determined to be around 8×10^(-2) mg/mL. The LCST behavior is studied using UV/Visible spectrophotometer. It is found that the LCST is concentration-dependent, and the variation of LCST is less significant at higher concentrations. The LCST range covers human body temperature. Preliminary drug loading and release experiments revealed good entrapment efficiency and instant release of drug at LCST phase change, showing potential of PPG-bis-adenine as a targeted drug carrier. | en |
dc.description.provenance | Made available in DSpace on 2021-05-14T17:42:21Z (GMT). No. of bitstreams: 1 ntu-104-R00524061-1.pdf: 2853004 bytes, checksum: b67d74ed9e83da55f55a6db5a3db4d63 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 誌謝 i
摘要 ii Abstract iii Table of Contents iv List of Figures vi List of Tables viii Abbreviations ix Chapter 1. Introduction 1 Chapter 2. Literature Review 2 2.1 Supramolecular Chemistry 2 2.2 Controlled Drug Release 4 2.2.1 The improvement of solubility for low-solubility drugs 4 2.2.2 Targeted delivery of drugs 5 2.3 Polymeric Nanoparticles 6 2.3.1 Hydrophobic Interaction 6 2.3.2 Shapes of aggregation of Amphiphilic Molecules 6 2.3.3 Amphiphilic Copolymers 8 2.3.4 Temperature-sensitive Copolymers as Carriers for Targeted Delivery 9 2.3.5 Drug loading and release 11 2.4 Poly(propylene glycol) 11 2.5 Nucleobases 12 Chapter 3. Materials and Methods 14 3.1 Synthesis of PPG-bis-adenine 14 3.1.1 The Addition Reaction 14 3.1.2 Purification by Column Chromatography 15 3.2 Nuclear Magnetic Resonance (NMR) 16 3.3 Gel Permeation Chromatography (GPC) 16 3.4 Elemental Analysis 17 3.5 Mass Spectroscopy (MALDI-TOF-TOF) 17 3.6 Critical Micelle Concentration (CMC) determination by pyrene fluorescence 18 3.7 Lower Critical Solution Temperature (LCST) determination by UV/Visible spectrophotometry 20 3.8 Mean diameter measurement by Dynamic Light Scattering (DLS) 22 3.9 Zeta potential measurement 23 3.10 Scanning Electron Microscopy (SEM) 23 3.11 Cryogenic Transmission Electron Microscopy (cryo-TEM) 24 3.12 Drug Loading and Release 25 Chapter 4. Results and Discussion 27 4.1 Structure Characterization 27 4.1.1 NMR Spectroscopy 27 4.1.2 Mass spectroscopy & GPC 27 4.1.3 Elemental analysis 33 4.2 Evidence of Nanoparticles 36 4.2.1 Dynamic light scattering 36 4.2.2 Scanning Electron Microscopy 36 4.2.3 Cryo-TEM 41 4.2.4 Zeta potential 41 4.2.5 CMC determination 42 4.3 The LCST behavior 45 4.3.1 LCST determination by UV/Vis spectrophotometry 45 4.3.2 Mean diameter change around LCST 45 4.4 Drug loading and release 48 Chapter 5. Conclusions and Future work 50 References 51 | |
dc.language.iso | en | |
dc.title | 功能性聚丙二醇-二腺嘌呤奈米顆粒性質研究 | zh_TW |
dc.title | Functional nanoparticles of Poly(propylene glycol)-bis-Adenine | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 鄭智嘉(Chih-Chia Cheng) | |
dc.contributor.oralexamcommittee | Christopher Whiteley(Christopher Whiteley),朱曉萍,黃志彬(Chih-Pin Huang) | |
dc.subject.keyword | 超分子,奈米顆粒,溫度感應,藥物輸送,聚丙二醇, | zh_TW |
dc.subject.keyword | supramolecular,nanoparticles,temperature-responsive,drug delivery,poly(propylene glycol), | en |
dc.relation.page | 56 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2016-01-05 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
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