請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44275
標題: | 菇蕈多醣體對卵蛋白致敏小鼠之影響 The Effects of β-glucan on OVA-sensitizes Mice |
作者: | Wei-Cheng Lin 林微錚 |
指導教授: | 陳秀男(Shiu-Nan Chen) |
關鍵字: | 過敏,多醣體,卵蛋白,th1,th2, allergy,glucan,OVA,ovalbumin, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | The aim of the experience is to understand mushroom polysaccharides can be prevented of allergy. The design of experiment continuous feeding different concentration of glucan for two weeks (0mg/200μl, 1mg/200μl, 2mg/200μl), and B6 mice were sensitized by OVA from twice to five times. Using different levels of sensitization to know the effect of polysaccharide feeding mice so we detected IFN-γ, IgG2a, IgE and IL-4 concentration in serum of the sensitization mice. Taking advantage of cytokines and immune globulin to study polysaccharides of the efficacy.
The results show that B6 mice sensitized several times, the group feeding mushroom polysaccharides of this experimental are eligible for a higher concentration of IFN-γ, and the group sensitized twice and four times by OVA, the experimental group and control group a comparison of significant differences (P <0.05). The IgG2a serum concentrations have similar results to IFN-γ, regardless of the sensitizion frequency,the group feeding glucan may be got higher IgG2a levels. The coparison of the polysaccharide treatment group and control group had a significant difference (P <0.05). The IgE concentrations in serum of feeding polysaccharides of B6 mice are lower than the control group, and the comparison of glucan treatmet and control had a significant difference (P <0.05) of the group sensitized twice to five times. The group fed 1mg/200μl of glucan had higher IL-4 concentrations in serum than any other treatment group or nontreayment froup, and there is significant difference (P <0.05). The result of IL-4 concentration in treatment group were lower than control group whether sensitization twice to five times, but no significant differences. The results show that mushroom polysaccharides can effectively enhance the IFN-γ and IgG2a concentration in the blood and reduce IgE levels, amount regardless of the number of allegens. Mushroom polysaccharides improved response to Th1cells, helps fight allergies, but the effects of IL-4 from mushroom polysaccharide is not obvious in this experiment. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44275 |
全文授權: | 有償授權 |
顯示於系所單位: | 漁業科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 2.08 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。