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標題: | 利用雙光子螢光和二倍頻顯微術觀察高血脂兔虹膜和心血管動脈硬化之正相關性 Utilizing Two-Photon Fluorescence and Second Harmonic Generation Microscopy to Observe the Correlation Between the Iris and Cardiovascular Atherosclerosis of Hypercholesterolemic Rabbits |
作者: | Hai-Wei Jao 饒海威 |
指導教授: | 董成淵(Chen-Yuan Dong) |
關鍵字: | 雙光子螢光顯微術,二倍頻,動脈硬化,高血脂,主動脈弓,虹膜,快速傅立葉轉換, Two photon microscopy,Second Harmonic Generation,Atherosclerosis,Hypercholesterolemia,Aortic arch,Iris,fast Fourier transform, |
出版年 : | 2008 |
學位: | 碩士 |
摘要: | 近年來由於高熱量食物愈來愈普遍,全球各地的罹患動脈硬化患者的年齡層逐年下降,尤其在這一、兩年動脈硬化更是西方國家研究的主題之一。而以目前的醫學技術對於動脈硬化的研究,主要是進行非活體的切片觀察其病變的程度,而少以非侵入式的方式給予患者即時的診斷並提早開始預防。
雙光子螢光顯微術是一種非侵入式的顯微術,具有低光破壞、低光漂白、較深的穿透深度及較佳的影像對比度等優點。 本實驗是將台大醫院所飼養的實驗兔,分成高血脂實驗組和正常的對照組,再利用雙光子螢光與二倍頻顯微術,觀察其虹膜及弓主動脈的病理切片,判斷其虹膜病變及弓主動脈硬化程度之相關性。最後再用雙光子顯微鏡觀察正常與病變的虹膜,在少了角膜覆蓋且尚未脫離眼球的情況下,掃出來的影像上的差異,並用快速傅立葉轉換做量化分析。 未來更期望能對活體的虹膜進行觀察,並發展成能對患者虹膜做非侵入式的即時診斷之醫療技術。 In recent years, due to the popularization of high caloric diet, the age of patients suffering from atherosclerosis became lower year by year all over the world. In recent years, atherosclerosis is even one of the most important research topics in Western countries. The medical study for atherosclerosis, however, relies primarily on the observation of ex vivo histological sections instead of observing the patients with intravital technique. Two-photon fluorescence microscopy is an intravital microscopy that has a number of advantages including lower photondamage, lower photon-bleaching, deeper penetrating distance, and higher imaging contrast. To test the ability of two-photon microscopy in the imaging and detection of atherosclerosis, we obtained and separated the experimental rabbits provided by National Taiwan University Hospital into two groups: hypercholesterolemic experimental group and normal control group. Next, we utilized the two-photon fluorescence microscopy and second harmonic generation (SHG) imaging to observe the correlation between the iris and aortic arch of hypercholesterolemic rabbits. We were able to observe differences between the images of normal and hypercholesterolemic iris on the eyeball and not covered by cornea. In addition, we analyzed and quantified the images with fast Fourier transform technique. In the future, we hope we could observe the iris in vivo and develop it into an intravital medical technology to diagnose the atherosclerosis level of the patients. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42067 |
全文授權: | 有償授權 |
顯示於系所單位: | 物理學系 |
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