請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42067
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 董成淵(Chen-Yuan Dong) | |
dc.contributor.author | Hai-Wei Jao | en |
dc.contributor.author | 饒海威 | zh_TW |
dc.date.accessioned | 2021-06-15T00:44:53Z | - |
dc.date.available | 2010-06-27 | |
dc.date.copyright | 2008-09-02 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-08-27 | |
dc.identifier.citation | 1. Kochanek, K.D., Murphy, S.L., Anderson, R.N. & Scott, C. Deaths: Final data for 2002. National Vital Statistics Reports 53, 1-115 (2004).
2. Hoyert, D.L. & Kochanek, K.D. Deaths: Final Data for 1997. National Vital Statistics Reports; 47 (1999). 3. Tuzcu, E.M. et al. 2705-2710 (Am Heart Assoc, 2001). 4. Michael C. Fishbein, M.D.L.J.S. (2007). 5. Roscoe, H.G. & Vogel, A.W. Lipid Changes in the Eye Concomitant with the Development of Atherosclerosis in the Aorta in the Rabbit. Circulation Research 23, 633-643 (1968). 6. Wu, C.C., Chang, S.W., Chen, M.S. & Lee, Y.T. Early Change of Vascular Permeability in Hypercholesterolemic Rabbits. Arteriosclerosis, Thrombosis, and Vascular Biology 15, 529-533 (1995). 7. Goeppert-Mayer, M. Uber Elementarakte mit zwei quantensprungen. Ann. Physik. 9, 273-294 (1931). 8. Franken, P.A., Hill, A.E., Peters, C.W. & Weinreich, G. Generation of Optical Harmonics. Physical Review Letters 7, 118-119 (1961). 9. Kaiser, W. & Garrett, C.G.B. Two-Photon Excitation in CaF2: Eu2+. Physical Review Letters 7, 229 (1961). 10. Singh, S. & Bradley, L.T. Three-Photon Absorption in Napthalene Crystals by Laser Excitation. Physical Review Letters 12, 612-614 (1964). 11. Denk, W., Strickler, J.H. & Webb, W.W. Two-photon laser scanning fluorescence microscopy. Science 248, 73-76 (1990). 12. Hsiao, C.Y. et al. Imaging Condition Optimization in Multiphoton Microscopy of Three-Dimensional Collagen Fiber Structures. Journal of the Chinese Chemical Society 51, 1115-1120 (2004). 13. Liu, Y. et al. Visualization of hepatobiliary excretory function by intravital multiphoton microscopy. Journal of Biomedical Optics 12, 014014 (2007). 14. Teng, S.W. et al. Multiphoton Autofluorescence and Second-Harmonic Generation Imaging of the Ex Vivo Porcine Eye. Investigative Ophthalmology & Visual Science 47, 1216-1224 (2006). 15. FC Li, C.W., SJ Lin, SH Jee, W Lo, CY Dong. Dorsal Skin Fold Chamber for High Resolution Multiphoton Imaging. Optical and Quantum Electronics (2005). 16. Lin, M. et al. Evaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy. Journal of Biomedical Optics 11, 64006 (2006). 17. Lee, J.N. et al. The Effects of Depilatory Agents as Penetration Enhancers on Human Stratum Corneum Structures. Journal of Investigative Dermatology (2008). 18. Chou, C.K. et al. Polarization ellipticity compensation in polarization second-harmonic generation microscopy without specimen rotation. Journal of Biomedical Optics 13, 014005 (2008). 19. Eriksson, E.E., Xie, X.U.N., Werr, J., Thoren, P. & Lindbom, L. Direct viewing of atherosclerosis in vivo: plaque invasion by leukocytes is initiated by the endothelial selectins. The FASEB Journal 15, 1149 (2001). 20. van Zandvoort, M. et al. Two-Photon Microscopy for Imaging of the (Atherosclerotic) Vascular Wall: A Proof of Concept Study. Journal of Vasc Res 41, 54-63 (2004). 21. Zoumi, A., Lu, X., Kassab, G.S. & Tromberg, B.J. Imaging Coronary Artery Microstructure Using Second-Harmonic and Two-Photon Fluorescence Microscopy. Biophysical Journal 87, 2778-2786 (2004). 22. Le, T.T., Langohr, I.M., Locker, M.J., Sturek, M. & Cheng, J.X. Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy. Journal of Biomedical Optics 12, 054007 (2007). 23. Lide, D.R. CRC handbook of chemistry and physics: A Ready-Reference Book of Chemical and Physical Data (CRC Press, 2004). 24. Griffiths, D.J. Introduction to Electrodynamics. Introduction to Electrodynamics 12 (1999). 25. Boyd, R.W. Nonlinear Optics (Academic Press, 2003). 26. Griffiths, D.J. & Harris, E.G. Introduction to Quantum Mechanics. American Journal of Physics 63, 767 (1995). 27. Shankar, R. Principles of Quantum Mechanics (Springer, 1994). 28. Ross, M.H., Kaye, G.I. & Pawlina, W. Histology: a text and atlas with cell and molecular biology (Lippincott Williams & Wilkins, Philadelphia, 2003). 29. Ross, R. The pathogenesis of atherosclerosis. Mech Ageing Dev 9, 435-40 (1979). 30. Ross, R. The pathogenesis of atherosclerosis--an update. N Engl J Med 314, 488-500 (1986). 31. Ross, R. The pathogenesis of atherosclerosis: a perspective for the 1990 s. Nature 362, 801-809 (1993). 32. Ross, R. Atherosclerosis is an inflammatory disease. American Heart Journal 138, S419 (1999). | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42067 | - |
dc.description.abstract | 近年來由於高熱量食物愈來愈普遍,全球各地的罹患動脈硬化患者的年齡層逐年下降,尤其在這一、兩年動脈硬化更是西方國家研究的主題之一。而以目前的醫學技術對於動脈硬化的研究,主要是進行非活體的切片觀察其病變的程度,而少以非侵入式的方式給予患者即時的診斷並提早開始預防。
雙光子螢光顯微術是一種非侵入式的顯微術,具有低光破壞、低光漂白、較深的穿透深度及較佳的影像對比度等優點。 本實驗是將台大醫院所飼養的實驗兔,分成高血脂實驗組和正常的對照組,再利用雙光子螢光與二倍頻顯微術,觀察其虹膜及弓主動脈的病理切片,判斷其虹膜病變及弓主動脈硬化程度之相關性。最後再用雙光子顯微鏡觀察正常與病變的虹膜,在少了角膜覆蓋且尚未脫離眼球的情況下,掃出來的影像上的差異,並用快速傅立葉轉換做量化分析。 未來更期望能對活體的虹膜進行觀察,並發展成能對患者虹膜做非侵入式的即時診斷之醫療技術。 | zh_TW |
dc.description.abstract | In recent years, due to the popularization of high caloric diet, the age of patients suffering from atherosclerosis became lower year by year all over the world. In recent years, atherosclerosis is even one of the most important research topics in Western countries. The medical study for atherosclerosis, however, relies primarily on the observation of ex vivo histological sections instead of observing the patients with intravital technique.
Two-photon fluorescence microscopy is an intravital microscopy that has a number of advantages including lower photondamage, lower photon-bleaching, deeper penetrating distance, and higher imaging contrast. To test the ability of two-photon microscopy in the imaging and detection of atherosclerosis, we obtained and separated the experimental rabbits provided by National Taiwan University Hospital into two groups: hypercholesterolemic experimental group and normal control group. Next, we utilized the two-photon fluorescence microscopy and second harmonic generation (SHG) imaging to observe the correlation between the iris and aortic arch of hypercholesterolemic rabbits. We were able to observe differences between the images of normal and hypercholesterolemic iris on the eyeball and not covered by cornea. In addition, we analyzed and quantified the images with fast Fourier transform technique. In the future, we hope we could observe the iris in vivo and develop it into an intravital medical technology to diagnose the atherosclerosis level of the patients. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T00:44:53Z (GMT). No. of bitstreams: 1 ntu-97-R95222059-1.pdf: 3890617 bytes, checksum: 6f45bc05f32f15c09be6954b0af859be (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 口試委員審定書 i
致謝 ii 中文摘要 iv Abstract v Table of Content vii Figure Catalog viii Table Catalog ix Chapter 1 Introduction 1 Chapter 2 Basic Principles 4 2.1 Second Harmonic Generation (SHG) 4 2.2 Two-Photon Fluorescence 10 Chapter 3 Histology of Iris and Aortic Arch 17 3.1 Histology of the Iris 17 3.2 Histology of the Aortic Arch 20 3.3 Pathogenesis of Atherosclerosis 22 3.4 Correlation between Iris and Atherosclerosis 24 Chapter 4 Experimental Setup, Animals, and Methods 25 4.1 Experimental Setup 25 4.2 Experimental Animal Models 28 4.3 Experimental Methods 29 Chapter 5 Results and Discussions 33 5.1 Comparation of Section Images 33 5.2 Direct View of Iridic Plaque 46 Chapter 6 Conclusions and Perspectives 58 References 59 | |
dc.language.iso | en | |
dc.title | 利用雙光子螢光和二倍頻顯微術觀察高血脂兔虹膜和心血管動脈硬化之正相關性 | zh_TW |
dc.title | Utilizing Two-Photon Fluorescence and Second Harmonic Generation Microscopy to Observe the Correlation Between the Iris and Cardiovascular Atherosclerosis of Hypercholesterolemic Rabbits | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張顏暉(Yuan-Huei Chang),李宣書(Hsuan-Shu Lee),林頌然(Sung-Jan Lin) | |
dc.subject.keyword | 雙光子螢光顯微術,二倍頻,動脈硬化,高血脂,主動脈弓,虹膜,快速傅立葉轉換, | zh_TW |
dc.subject.keyword | Two photon microscopy,Second Harmonic Generation,Atherosclerosis,Hypercholesterolemia,Aortic arch,Iris,fast Fourier transform, | en |
dc.relation.page | 60 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-08-27 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 物理研究所 | zh_TW |
顯示於系所單位: | 物理學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-97-1.pdf 目前未授權公開取用 | 3.8 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。