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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 游若? | |
dc.contributor.author | Fang-jung Chu | en |
dc.contributor.author | 朱芳瑢 | zh_TW |
dc.date.accessioned | 2021-06-14T16:41:47Z | - |
dc.date.available | 2011-08-08 | |
dc.date.copyright | 2008-08-08 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-08-01 | |
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Dairy Sci. 1991, 74, 1477-1481. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40145 | - |
dc.description.abstract | 結腸直腸癌為國人主要的癌症死因,約有70 % 的結腸直腸癌與飲食有關。近年來益生菌的相關研究指出,益生菌及其製品對致突變劑和致突變原具有抗致突變和抗腫瘤等功效,因此本研究以過去本實驗室篩選出之具有較佳耐酸性pH、膽汁及對 B[a]P、t-BOOH 與 H2O2 抗致突變性較好之五株益生菌為材料,以 MTT 細胞毒性試驗檢測其抑制人類結腸癌細胞株 HT-29 及 Caco-2 增生及4-nitroquinoline-N-oxide (4NQO) 對腸道細胞 Int-407 細胞毒性之效果,並進一步利用分段式預反應探討其抗致突變的機制。此外,利用彗星電泳法 (comet assay) 檢測益生菌抑制4NQO誘導Int-407基因毒性之效果。結果顯示益生菌於高濃度 (108 CFU/mL) 時,抑制大腸癌細胞增生之效果較低濃度 (106 CFU/mL) 好。且隨著作用時間增加至48小時,抑制癌細胞增生之效果也更顯著,其中以108 CFU / mL 之 Bifidobacterium lactis Bb-12 的粗細胞壁抑制效果最佳,與 HT-29 及 Caco-2 作用 48 小時後之細胞存活率分別為 70.6 % 及 63.3 %。在抑制基因毒性方面,B. lactis Bb-12與L. casei 01 的熱致死細胞降低由 4NQO 所誘導的 Int-407 細胞 DNA 損傷情形最佳。在抗細胞毒性的機制方面,部分益生菌之粗細胞壁對 4NQO 具有去致突變 (desmutagenic effect) 的效果,其存活率可由 56.3 % 上升至 66.4 %;而益生菌之胞內液則具有阻斷作用 (blocking effect),其細胞存活率可由 57.4 % 上升至 65.8 %。此外,除了益生菌菌體本身外,以 B. lactis Bb-12 及 L. casei 01 所發酵之牛乳上清液同樣可以抑制 4NQO 誘導 Int-407 之 DNA 損傷及細胞毒性,其抑制細胞毒性之機制同樣為去致突變及阻斷作用,其中去致突變之抑制率分別為 37.4 % 與 52.5 %,而阻斷作用之抑制率則分別為 34.1 % 與 41.4 %。 | zh_TW |
dc.description.abstract | Colorectal cancer is a major cause of death from cancer in Taiwan. Approximately 70 % of colorectal cancer is related to dietary habit. Many researches showed that probiotics, both cellular compositions and fermented products have antimutagenic and antitumor activities on mutagens. In present study, we used several probiotics, which have been studied in our laboratory for their acid-resistance, bile salt tolerance, and antimutagenicity against B[a]P, t-BOOH, and H2O2. In addition, MTT assay was used to explore the effects of several probiotics against 4NQO cytotoxicity on Int-407 and the proliferation of human colon cancer cell HT-29 and Caco-2. Furthermore, possible antimutagenic mechanisms of probiotics were investigated by section-preincubation test and comet assay to explore the effects of several probiotics against 4NQO-induced DNA damage on Int-407. The results showed that a high probiotics population inhibits colon cancer cell more effectively than a low probiotics population. Among the strains, the crude cell walls of Bifidobacterium lactis Bb-12 exhibited the most potent inhibition activity in HT-29 and Caco-2 for 48h and the viability were 70.6 % and 63.3 %, respectively. The heat-killed cells of B. lactis Bb-12 and L. casei 01 significantly reduced the genotoxicity of 4NQO-induced DNA damage on Int-407 (p < 0.05). In addition, the crude cell walls and intracellular extracts of probiotics showed inhibitory effects against 4NQO cytotoxicity. The primary mechanism of crude cell walls was desmutagenic effect and intracellular extracts was blocking effect. Besides, fermented milk with B. lactis Bb-12 and L. casei 01 also had inhibition on 4NQO-induced DNA damage and cytotoxicity on Int-407. Their main mechanisms were the same as above, inhibition rate were 37.4 % - 52.5 % and 34.1 % - 41.4 %, respectively. | en |
dc.description.provenance | Made available in DSpace on 2021-06-14T16:41:47Z (GMT). No. of bitstreams: 1 ntu-97-R95641020-1.pdf: 1374559 bytes, checksum: b62b7245bc28bbeed5b1f7963fb14a1e (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii 英文摘要 iii 目錄 iv 圖目錄 vi 表目錄 ix 壹、前言 1 貳、文獻整理 2 一、益生菌 2 (一)益生菌之定義 2 (二)乳酸菌與雙叉桿菌 2 (三)益生菌之健康功效 3 (四)益生菌之安全性評估 10 二、膳食中的致突變/致癌劑 11 三、膳食中的抗致突變/致癌劑 14 四、抗致突變物/致癌物之作用機制 16 (一)去致突變作用(desmutagenic effect) 18 (二)阻斷作用(blocking effect) 18 (三)生物抗致突變作用(bioantimutagenic effect) 18 五、大腸癌 18 (一)大腸癌之形成 18 (二)影響大腸癌之膳食因子 19 (三)益生菌與結腸癌的關係 22 六、4-Nitroquinoline N-oxide (4NQO) 之致突變性 23 參、材料與方法 26 一、實驗架構 26 二、實驗材料 28 (一) 試驗菌株 28 (二) 試驗細胞株 28 (三) 藥品 28 (四) 器材 29 三、試驗方法 29 (一) 菌種的活化與保存 29 (二) 細胞株的活化、繼代培養與保存 30 (三) 益生菌之熱致死細胞、粗細胞壁、胞內液及發酵乳上清液之製備 31 (四) MTT assay 32 (五) 益生菌及其發酵乳抑制人類結腸癌細胞株 HT-29 及 Caco- 2增生之試驗 32 (六)益生菌及其發酵乳對人類腸道細胞 Int-407 存活率的影響 33 (七) 益生菌及其發酵乳上清液抑制 4NQO 對 Int-407 之細胞毒性試驗 33 (八) 彗星試驗 (Comet assay) 34 (九) 益生菌及其發酵乳上清液抑制 4NQO 對 Int-407 細胞毒機制之探討 35 (1)益生菌及其發酵乳上清液的去致突變效應 (Desmutagenic effects) 35 (2)益生菌及其發酵乳上清液的阻斷效應 (Blocking effects) 35 (3)益生菌及其發酵乳上清液的生物抗致突變效應 (Bioantimutagenic effects) 36 (十) 統計分析 36 肆、結果與討論 37 一、益生菌對 HT-29 及 Caco-2 細胞株的抑制效果 37 二、益生菌之毒性試驗 45 三、益生菌抑制4NQO誘導之細胞毒性 45 四、益生菌抑制4NQO誘導之基因毒性 47 五、益生菌抑制 4NQO 誘導細胞毒性機制之探討 53 (1) 益生菌的去致突變作用 (Desmutagenic effect) 53 (2) 益生菌對 4NQO 的阻斷作用 (Blocking effect) 55 (3) 益生菌的生物抗致突變作用 (Bioantimutagenic effect) 55 六、益生菌發酵牛乳之上清液對 HT-29 及 Caco-2 細胞株的抑制效果 58 七、益生菌發酵牛乳之上清液抑制 4NQO 誘導之細胞毒性及基因毒性 59 (1) 發酵乳上清液抑制 4NQO 誘導之細胞毒性 59 (2) 益生菌發酵牛乳之上清液抑制 4NQO 誘導之基因毒性 62 八、益生菌發酵牛乳之上清液抑制 4NQO 誘導 Int-407 細胞毒性機制之探討 64 伍、結論 68 陸、參考文獻 69 附錄 (遵照臺灣農業化學與食品科學期刊之稿約撰寫) 81 | |
dc.language.iso | zh-TW | |
dc.title | 數株益生菌及其發酵乳抑制大腸癌細胞增生及4NQO誘導Int-407基因毒性之研究 | zh_TW |
dc.title | Inhibition of colon cancer cell proliferation and genotoxicity of 4-nitroquinoline-N-oxide on human intestine cell by several probiotics and fermented milk | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 丘志威,李?鈴,顏聰榮,周正俊 | |
dc.subject.keyword | 益生菌,4-Nitroquinoline-N-oxide,彗星電泳,去致突變,阻斷作用, | zh_TW |
dc.subject.keyword | probiotics,4-Nitroquinoline-N-oxide,comet assay,desmutagenic effect,blocking effect, | en |
dc.relation.page | 100 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-08-01 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
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