微混合器與共振式微懸臂梁生物感測器 的理論建立與數值模擬

Abstract

最近二十年，微混合器與生物感測器被廣泛的研究與發展。微混合器主要是運用力學機制來混合微流道內不同流體的一種裝置。而生物感測器則是因為具備了高靈敏度、微量檢測及即時偵測等優異特性，因此提供了即時且便利的生物檢測方式。 在微流道系統中，交流電動力往往被當作操控粒子在流體中運動以及擾動流場的一種機制，交流電動力主要包含了介電泳、交流電滲，以及電熱力等，而這些交流電動力，則會因為懸浮物的帶電量，電解溶液的導電度，以及交流電場電壓的不同等而有所區分。透過交流電場的施加，流場內的懸浮粒子將能被有效的操控，或者流場因電動力所產生的渦流，能被有效的攪拌。 本論文主要分成三個部分：微混合器，共振式微懸臂梁生物感測器以及生物感測器的免疫分析。在微混合器與生物感測器的免疫分析方面，由有限元素法的模擬方式，利用交流電熱力對其效能上的改善；在共振式微懸臂梁生物感測器方面，將透過理論與模擬來對其作一廣泛性的討論，包含其基本振動特性的研究，共振式微懸臂梁生物感測器的靈敏度討論與改良，以及不同型式的共振式生物感測器的比較等，而主要對象元件則包括，單一微懸臂梁生物感測器、微懸臂梁陣列，懸臂式微流道生物感測器，以及石英晶體微天平。

In the last two decades, micromixers and biosensors in the micro system are popularly investigated and developed. A micromixer is primarily employed for mixing fluids by means of externally or internally mechanical mechanisms. A biosensor is based on several advantages, such as highly sensitive, tiny mass load detection and capable of monitoring dynamic biomolecular interaction in real time, to be used as the apparatus for the biomolecule detection. Then, the study of AC electrokinetics is correlated with the motion behavior of particles in fluids, and it is also widely applied on microfluidic devices in the recent decade. When microfluidic devices of the microfabricated electrode systems are subjected to AC electrical fields, the AC electrokinetics involving the dielectrophoresis, AC electroosmosis or AC electrothermal force will be generated according to charges of colloids, different properties of electrolytes or amplitude and frequency of external applied voltages. This thesis is divided into three major subjects: micromixers, resonant microcantilever-based biosensors, and immunoassay of the biosensor. In terms of AC electrokinetics: AC electrothermal force, micromixers and immunoassay of the biosensor will be concentrated on meliorating their working efficiency by employing finite element method. For resonant microcantilever-based biosensors, a comprehensive investigation will be theoretically and numerically executed, involving the study of fundamental vibrational properties, the sensitivity of resonant microcantilever-based biosensors, and a comparison of distinct resonant biosensors, etc. Target components comprise as follows: micorcantilever beam sensor (MCB), microcantilever beam array sensor, suspended microchannel resonator (SMR), quartz crystal micorbalance (QCM).