神經動作電位無線記錄系統

Abstract

本研究為神經動作電位無線記錄之初期研究，共分為兩部份：神經動作無線記錄系統與大鼠心率計量器之開發。實驗室目前電生理信號記錄系統是侷限於有線傳輸階段，連接電纜線至電極以記錄大鼠腦部受外部刺激之相關反應，本研究使用無線傳輸技術取代原本電生理實驗有線傳輸的方式，並且整合類比/數位轉換功能發展出高通用性的記錄系統。心率計量器的設計是為了能在電生理實驗中即時反應大鼠心跳次數而發展出一套麻醉深度輔助監控系統。 本系統為單通道神經動作電位無線記錄系統，結合了信號調控電路、外部觸發、類比/數位轉換以及藍芽傳輸等功能，可以應用於電生理實驗記錄與錄音帶資料備份記錄。依據不同實驗情況的電生理信號記錄，可修改微控制器韌體並將取樣頻率、傳輸速度以及資料擷取時間長度調整至實驗需求。 根據實際動物實驗量測，神經動作電位無線記錄系統與原本有線傳輸記錄系統所記錄到神經動作電位其相關係數高達0.999，証實本研究所開發的無線傳輸記錄器能取代原本的記錄系統，而大鼠心率計量器的監控也能得知麻醉深度是否適當，以維持電生理信號的穩定性。透過本研究的初步評估，本實驗室能基於此架構再繼續進行另一階段的多通道神經動作電位無線記錄系統的研究。

The thesis is a pilot research of wireless recording system for action potentials. The system includes wireless recording system and heart rate meter (HR meter). In traditional recording system, evoked responses recording was transmitted through the cable. In this research, wireless recording system which includes wireless technique and A/D (Analog-to-Digital) converter was proposed to substitute the traditional cable transmissions. Furthermore, HR meter was designed for monitoring the anesthetic level of rat through detecting the heart rate with real-time display due to it affects the S/N ratio of electrophysiological signal seriously. The single-channel wireless recording system consists a precondition unit, external trigger, A/D converter, and Bluetooth module. This system can be used for simulation, off-line sampling and real-time electrophysiological recording. The microcontroller firmware that controls the sampling rate, baud rate and acquisition time could be adjustable for user according to different experimental requirements. After the actual experimental measurement, the correlation-coefficient between cable transmission and wireless recording is 0.999. HR meter that monitors the heart rate can help us to monitor the level of anesthesia of the rat during experiment. By use of this system, we might evaluate the feasibility of multi-channel wireless recording system in the future.