聚四氟乙烯及聚甲基丙烯酸甲酯之改質與生物相容性評估及運用

Abstract

本實驗利用丁基鋰化合物在不同推電子溶劑(乙二胺、丁二胺及乙胺)催化下，改質高分子聚四氟乙烯(PTFE)，使其產生去氟烷化反應；利用FTIR-ATR、ESCA分析改質前後表面化學組成差異並測量接觸角以判斷親疏水性變化。 同時利用體外細胞培養的方法，來探討顆粒型神經細胞在改質前後PTFE的適合性，並且利用微流道技術設計細胞圖案，使PTFE產生親疏水性差異的表面。結果使用金相顯微鏡及掃描式電子顯微鏡觀察細胞生長。由結果得知，未改質前細胞無法貼附生長，在經由改質後之PTFE表面可以發現細胞群聚成球且生長突觸的現象。

In this study, butyllithium was catalyzed by different electron-donating solvents (ethyldiamine, butyldiamine and ethylamine) for defluorination and alkylation to modify PTFE. FTIR-ATR and a ESCA results were used to analyze the composition of unmodified / modified surfaces and contact angles were measured to distinguish the variation of hydrophobicity. In vitro cerebellum cortical granule cells culturing was then used to test the biocompatibility of PTFE before and after modification, Microfluidic channels were also used to produce differential hydrophilic surfaces for cell patterning. Finally, a Metallurgical Microscope and a SEM were used to observe cell growing. According to the experimental results, cells could not adhere to unmodified PTFE surfaces. On the other hand, small populations of cells and synapse extension were observed on the modified PTFE surface.