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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王廷方(Ting-Fang Wang) | |
| dc.contributor.author | Chun-Hsu Cheng | en |
| dc.contributor.author | 鄭純絮 | zh_TW |
| dc.date.accessioned | 2021-06-13T04:30:05Z | - |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-20 | |
| dc.identifier.citation | Agarwal, S. and Roeder, G.S. 2000. Zip3 provides a link between recombination enzymes and synaptonemal complex proteins. Cell 102: 245-255.
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J Biol Chem 278: 44113-44120. Chua, P.R. and Roeder, G.S. 1998. Zip2, a meiosis-specific protein required for the initiation of chromosome synapsis. Cell 93: 349-359. Hannich, J.T., Lewis, A., Kroetz, M.B., Li, S.J., Heide, H., Emili, A., and Hochstrasser, M. 2005. Defining the SUMO-modified proteome by multiple approaches in Saccharomyces cerevisiae. J Biol Chem 280（6）: 4102-4110. Henderson, K.A. and Keeney, S. 2004. Tying synaptonemal complex initiation to the formation and programmed repair of DNA double-strand breaks. Proc Natl Acad Sci U S A 101（13）: 4519-4524. -. 2005. Synaptonemal complex formation: where does it start? Bioessays 27（10）: 995-998. Hunter, N., and Kleckner, N. （2001）. The single-end invasion: an asymmetric intermediate at the double-strand break to double-holliday junction transition of meiotic recombination. Cell 106, 59-70. Johnson, E.S. 2004. Protein modification by SUMO. Annu Rev Biochem 73: 355-382. Johnson, E.S. and Gupta, A.A. 2001. An E3-like factor that promotes SUMO conjugation to the yeast septins. Cell 106: 735-744. Johnson, E.S. and Gupta, A.A. 2001. An E3-like factor that promotes SUMO conjugation to the yeast septins. Cell 106: 735-744. Klein, F., Laroche, T., Cardenas, M.E., Hofmann, J.F., Schweizer, D., and Gasser, S.M. 1992. Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast. J Cell Biol 117: 935-948. Keeney, S., Giroux, C. N., and Kleckner, N. （1997）. Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family. Cell 88, 375-384. Knop, M., Siegers, K., Pereira, G., Zachariae, W., Winsor, B., Nasmyth, K., and Schiebel, E. 1999. Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines. Yeast 15: 963-972. Li, S.J. and Hochstrasser, M. 2000. The yeast ULP2 （SMT4） gene encodes a novel protease specific for the ubiquitin-like Smt3 protein. Mol Cell Biol 20: 2367-2377. 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Curr Biol 11: 88-97. Song, J., Durrin, L.K., Wilkinson, T.A., Krontiris, T.G., and Chen, Y. 2004. Identification of a SUMO-binding motif that recognizes SUMO-modified proteins. Proc Natl Acad Sci U S A 101: 14373-14378. Stead, K., Aguilar, C., Hartman, T., Drexel, M., Meluh, P., and Guacci, V. 2003. Pds5p regulates the maintenance of sister chromatid cohesion and is sumoylated to promote the dissolution of cohesion. J Cell Biol 163: 729-741. Sym, M., Engebrecht, J.A., and Roeder, G.S. 1993. ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis. Cell 72: 365-378. Takahashi, Y., Toh, E.A., and Kikuchi, Y. 2003. Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro. J Biochem （Tokyo） 133: 415-422. Taylor, D.L., Ho, J.C., Oliver, A., and Watts, F.Z. 2002. Cell-cycle-dependent localisation of Ulp1, a Schizosaccharomyces pombe Pmt3 （SUMO）-specific protease. J Cell Sci 115: 1113-1122. Tsubouchi, T. and Roeder, G.S. 2005. A synaptonemal complex protein promotes homology-independent centromere coupling. Science 308: 870-873. Tung, K.S. and Roeder, G.S. 1998. Meiotic chromosome morphology and behavior in zip1 mutants of Saccharomyces cerevisiae. Genetics 149: 817-832. van Heemst, D., Kafer, E., John, T., Heyting, C., van Aalderen, M., and Zickler, D. 2001. BimD/SPO76 is at the interface of cell cycle progression, chromosome morphogenesis, and recombination. Proc Natl Acad Sci U S A 98: 6267-6272. Wang, T.F., Kleckner, N., and Hunter, N. 1999. Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction. Proc Natl Acad Sci U S A 96: 13914-13919. Worland, S.T. and Wang, J.C. 1989. Inducible overexpression, purification, and active site mapping of DNA topoisomerase II from the yeast Saccharomyces cerevisiae. J Biol Chem 264: 4412-4416. Yu, H.G. and Koshland, D.E. 2003. Meiotic condensin is required for proper chromosome compaction, SC assembly, and resolution of recombination-dependent chromosome linkages. J Cell Biol 163（5）: 937-947. Zhao, X. and Blobel, G. 2005. A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization. Proc Natl Acad Sci U S A 102: 4777-4782. Zickler, D. and Kleckner, N. 1999. Meiotic chromosomes: integrating structure and function. Annu Rev Genet 33: 603-754. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/33225 | - |
| dc.description.abstract | 減數分裂是真核生物進行有性生殖時特化的細胞週期，主要功能再促進親代染色體間發生DNA重組互換，並確保染色體正確分離產生染色體數目減半的配子細胞（如精子與卵）。為了達到此目的，在減數分裂的前期中段染色體會形成聯會絲複合（synaptonemal complex），使來自父母雙方同源染色體之間緊密配對，促進DNA重組發生於同源染色體間。聯會絲複合體迄今已發現剛好五十年，但其形成的機制還不清楚，是有性生殖研究最重要課題之一，本論文的研究結果，對此課題有開創性的新發現。生物學界最常用來研究聯會絲複合體形成機制的生物模式是酵母菌，其Zip1蛋白質是建構聯會絲複合體的主要結構蛋白，Zip1也在減數分裂前期初段時，負責將兩對非同源染色體著絲點間偶合在一起，此機制與同源染色體間DNA重組並無直接的關聯性，到了減數分裂前期中段時，減數分裂專一性蛋白質Zip3負責啟動聯會絲複合體的形成。本論文證明Zip3是類泛素（Smt3）的E3接合酵素，負責在減數分裂前期中段時，將染色體上的結構蛋白質（如Red1）進行類泛素修飾。我們也發現Zip1能結合被類泛素化修飾的染色體結構蛋白質（如Top2），在減數分裂前期初期負責非同源染色體著絲點間的偶合，然後在減數分裂前期中段促進聯會絲複合體的形成。 | zh_TW |
| dc.description.abstract | Synaptonemal complex （SC） is a proteinaceous complex that apparently mediates synapsis between homologous chromosomes during meiotic prophase. In S. cerevisiae, the Zip1 protein is the integral component of SC. In the absence of a DNA double-strand break or the SC initiation protein Zip3, Zip1 proteins aggregate to form a polycomplex （PC）. In addition, Zip1 is also responsible for DSB-independent non-homologous centromere coupling at early meiotic prophase. We report here that Zip3 is a SUMO E3 ligase and that Zip1 is a binding protein for SUMO-conjugated products. Our results also suggest that at early meiotic prophase, Zip1 interacts with Zip3-independent Smt3 conjugates （e.g., Top2） to promote non-homologous centromere coupling. At and after mid-prophase, the Zip1 protein begins to associate with Zip3-dependent Smt3 conjugates （e.g., Red1） along meiotic chromosomes in the wild-type cell to form SCs and with Smt3 polymeric chains in the zip3 mutant to form PCs. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T04:30:05Z (GMT). No. of bitstreams: 1 ntu-95-R93b46011-1.pdf: 5586848 bytes, checksum: 73d32e07ec88b123b11a316313a1e695 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 口試委員會審定書
誌謝 中文摘要 5 英文摘要 Abstract 6 縮寫 7 第一章 序論 1.1 減數分裂的重要性 9 1.2 減數分裂DNA重組機制 9 1.3 減數分裂染色體型態變化 11 1.4 sumoylation對細胞的影響 14 第二章 材料與方法 2.1 酵母菌基因轉型 17 2.2酵母菌減數分裂細胞週期的同步化和孢子形成機率計算 18 2.3三氯乙酸蛋白質沉澱法製備酵母菌全細胞蛋白樣品 19 2.4蛋白質電泳與西方印跡術 19 2.5酵母菌雙雜合分析 20 2.6大腸桿菌重組蛋白質純化 21 2.7酵母菌重組蛋白質純化 24 2.8 In vitro 類泛素多聚合體分析 26 2.9 去磷酸化分析 27 2.10 Zip1C’末端接合分析 27 2.11實驗菌株 29 2.12實驗載體 33 2.13實驗培養基 36 第三章 結果 3.1 zip3突變菌株進入減數分裂時，在前期中段時期，類泛 素會形成多聚合體（Smt3 polymeric chain）。 38 3.2 Zip3是類泛素接合酶的新成員。 39 3.3 Zip1會與類泛素化產物結合。 42 第四章 討論 45 第五章 結果與圖表 圖一 觀察野生型和ulp2突變株在減數分裂時類泛素化 蛋白質的表現 47 圖二 觀察野生型和zip1-3突變株在減數分裂時類泛素化 蛋白質的表現 48 圖三 離胺酸突變類泛素在zip3突變菌株行減數分裂時的 蛋白表現 49 圖四 Zip3是類泛素接合酶的新成員 50 圖五 Zip3具有類泛素接合酶的活性 52 圖六 類泛素多元聚合體形成需要Zip1蛋白質 54 圖七 Zip1蛋白質C’末端具有類泛素結合模體 55 圖八 Zip1蛋白質會和被類泛素化的產物結合 56 表一 Zip3會專一性的與Ubc9和Smt3結合 51 第六章 參考文獻 57 第七章 附錄 附錄一 SUMO Modifications Control Assembly of Synaptonemal Complex and Polycomplex in Meiosis of Saccharomyces cerevisiae Cheng and Lo, et al. Gene and Development, 2006 附錄二 SUMO-mediated regulation of synaptonemal complex formation during meiosis. Carlos Egydio dde Carvalho and Monica P. Colaiacovo, 2006 | |
| dc.language.iso | zh-TW | |
| dc.subject | 類泛素化 | zh_TW |
| dc.subject | 減數分裂 | zh_TW |
| dc.subject | 聯會絲複合體 | zh_TW |
| dc.subject | meiosis | en |
| dc.subject | sumoylation | en |
| dc.subject | synaptomenal complex | en |
| dc.title | 類泛素轉譯後修飾作用調控減數分裂
同源染色體聯會絲複合體形成 | zh_TW |
| dc.title | SUMO Modifications Control Assembly of
Synaptonemal Complex in Meiosis of Saccharomyces cerevisiae | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 薛一蘋(Yi-Ping Hsueh),冷治湘(Chih-Hsiang Leng) | |
| dc.subject.keyword | 減數分裂,聯會絲複合體,類泛素化, | zh_TW |
| dc.subject.keyword | meiosis,synaptomenal complex,sumoylation, | en |
| dc.relation.page | 59 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-21 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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