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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 陳燕惠 | |
| dc.contributor.author | Zhan-Rong Li | en |
| dc.contributor.author | 李展榮 | zh_TW |
| dc.date.accessioned | 2021-06-13T04:18:12Z | - |
| dc.date.available | 2011-08-03 | |
| dc.date.copyright | 2006-08-03 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-24 | |
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Urban-Klein, B., Werth, S., Abuharbeid, S., Czubayko, F. and Aigner ,A., RNAi- mediated gene-targeting through systemic application of polyethylenimine (PEI)-complexed siRNA in vivo. Gene Ther, 2005. 12(5): p.461-6. 33. Ludwig, S., Planz, O., Pleschka, S. and Wolff, T., Influenza-virus-induced signaling cascades: targets for antiviral therapy? Trends Mol Med, 2003. 9(2): p.46-52. 34. Li, KS., Guan, Y., Wang, J., Smith, GJ., Xu, KM., Duan, L., Rahardjo, AP., Puthavathana, P., Buranathai, C., Nguyen, TD., Estoepangestie, AT., Chaisingh, A., Auewarakul, P., Long, HT., Hanh, NT., Webby, RJ., Poon, LL., Chen, H., Shortridge, KF., Yuen, KY., Webster, RG. and Peiris, JS., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia. Nature, 2004. 430(6996): p.209-13. 35. 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Chang, SF., Chang, HY., Tong, YC., Chen, SH., Hsaio, FC., Lu, SC. and Liaw, J., Nonionic polymeric micelles for oral gene delivery in vivo. Hum Gene Ther, 2004. 15(5): p.481-93. 40. Liaw, J., Chang, SF. and Hsiao, FC., In vivo gene delivery into ocular tissues by eye drops of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) polymeric micelles. Gene Ther, 2001. 8(13): p.999-1004. 41. Togo, T., Alderton, JM., Bi, GQ. and Steinhardt, RA., The mechanism of facilitated cell membrane resealing. J Cell Sci, 1999. 112 ( Pt 5): p.719-31. 42. Werth, S., Urban-Klein, B., Dai, L., Hobel, S., Grzelinski, M., Bakowsky, U., Czubayko, F. and Aigner, A.. A low molecular weight fraction of polyethylenimine (PEI) displays increased transfection efficiency of DNA and siRNA in fresh or lyophilized complexes. J Control Release, 2006. 112(2): p.257-70. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32888 | - |
| dc.description.abstract | 核醣核酸干擾(RNA interference , RNAi )是近來發展的新技術，可以用來抑制特定的基因表現。許多研究利用RNAi來抑制病毒的複製，都得到不錯的效果，使得RNAi有相當高的潛力成為新的抗病毒藥物。
目前已經找到不少運送RNAi的載體，但是大多數效果很好的載體都有毒性過高的缺點，所以本研究針對低毒性的分子來尋找可能的載體。 Pluronic 是屬於非離子性的介面活性劑，毒性不高。我們利用Pluronic類的聚合物 F68 、F108與L44來運送GAPDH siRNA，實驗結果並沒有看到GAPDH受到抑制。接著我們利用合成以共價鍵連接Pluronic F68與分子量2000Da的PEI。實驗結果顯示以Pluronic F68-PEI (2K)來運送GAPDH siRNA，有觀察到GAPDH表現受到抑制，而對照組PEI (2K)則沒觀察到GAPDH受到抑制。另外使用Pluronic F108-PEI (2K)來運送siRNA的實驗中則沒有看到GAPDH表現受到抑制。 | zh_TW |
| dc.description.abstract | Small interference RNA ( siRNA ) is a new technology and is able to inhibit specific gene expression. SiRNA has been used to inhibit the replication of viruses in many studies and becomes a new potential antiviral agent.
Many siRNA carriers have been developed. However, the more effective the carriers are, the higher toxicity they exert. Pluronic, a non-ionic surfactant with low toxicity, is considered as a carrier candidate in this study. There was no inhibition of GAPDH expression when Pluronic F68, F108 or L44 alone was used to deliver GAPDH siRNA. The Pluronic-grafted polyethyleneimine was then synthesized. The Pluronic F68-PEI (2K) moderately delivered GAPDH siRNA into BEAS-2B cells without detectable toxicity during transfection, while free PEI (2K) didn’t show the ability to deliver siRNA. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T04:18:12Z (GMT). No. of bitstreams: 1 ntu-95-R92423020-1.pdf: 720242 bytes, checksum: d26504359a3f4731e305449d6bfe6010 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 目錄
序論 - 1 第一節、RNA干擾 (RNA interference) - 2 第二節、DNA與RNAi的運送 - 5 第三節、流行性感冒病毒 - 9 實驗材料與方法 - 12 一、細胞培養基配製與BEAS-2B細胞培養 - 13 二、siRNA的製備 - 14 三、Pluronic 分子之臨界微膠體濃度(CMC)測量 - 15 四、Pluronic或Pluronic-PEI分子與siRNA結合之試驗 - 15 五、MTT assay - 16 六、Pluronic 分子進行轉染(transfection)之流程 - 17 七、Pluronic F108與Lipofectamine 2000 共同轉染 - 18 八、西方墨點法 (Western blotting) - 19 九、Pluronic分子與PEI分子接和反應 - 22 十、Pluronic-PEI以陽離子交換樹脂純化 - 24 十一、PEI偵測與定量(ninhydrin test) - 25 十二、Pluronic-PEI分子轉染(transfection) - 25 實驗結果 - 27 第一節、Pluronic分子特性與轉染(transfection)效果 - 28 第二節、Pluronic-PEI(2K)其分子性質與轉染效果 - 32 實驗討論 - 37 第一節、Pluronic性質與轉染效果討論 - 38 第二節、PluronicF108與Lipofectamine之共同轉染 - 39 第三節、Pluronic-PEI性質與轉染效果討論 - 40 圖表 - 44 參考文獻 - 61 | |
| dc.language.iso | zh-TW | |
| dc.subject | 聚合物 | zh_TW |
| dc.subject | 運送 | zh_TW |
| dc.subject | 小型干擾核醣核酸 | zh_TW |
| dc.subject | 微膠體 | zh_TW |
| dc.subject | siRNA | en |
| dc.subject | micelle | en |
| dc.subject | delivery | en |
| dc.subject | RNAi | en |
| dc.title | 以聚合物作為載體運送小型干擾核醣核酸之研究 | zh_TW |
| dc.title | Study of the polymers as vectors for delivery of siRNA into cells. | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳擇銘,邱士娟 | |
| dc.subject.keyword | 運送,聚合物,小型干擾核醣核酸,微膠體, | zh_TW |
| dc.subject.keyword | delivery,RNAi,siRNA,micelle, | en |
| dc.relation.page | 66 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-25 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 藥學研究所 | zh_TW |
| Appears in Collections: | 藥學系 | |
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