探討meso-substituted porphyrins上不同的 取代基對於細胞攝取、細胞內分佈位置 以及光動力治療效果的影響

Abstract

近年來，有關於研發更有效率的光感藥物應用在光動力治療(PDT)上，偏向針對於光感藥物的物化性質探討，像是吸收更往紅光區位移或是singlet oxygen的產生效率以及控制光感藥物在腫瘤細胞內分佈的位置 。根據它們的物化性質和細胞攝取機制，光感藥物能達到不同的細胞內給藥濃度和細胞內分佈位置。而且，若光感藥物能選擇性地分佈在目標胞器 ,例如線粒體或高基氏體 ,就能夠決定細胞的死亡路徑。 在我的研究中發現到，光感藥物的結構與它的傳遞機制和細胞攝取有緊密的正相關。而且經過ROS的偵測，可以發現隨著給藥時間的增加，細胞攝取也增加而且更多的ROS也隨之被偵測到。

The search for new efficient photosensitizers in photodynamic therapy (PDT) points to improve photophysical properties like absorption in the red region and singlet oxygen quantum yield as well as to control the localization of the sensitizer within the tumor cell. Depending on their physicochemical properties and their uptake mechanism, photosensitizers can reach different intracellular concentrations and localize in different subcellular compartments . Moreover, the preferential localization of a photosensitizer in target organelles, such as mitochondria or Golgi apparatus, can determine the cell death mechanism after PDT. The intracellular uptake was found to be closely related to the molecular structure of sensitizers and its subcellular trafficking in our study. Furthermore, Our result also revealed that more ROS were detected according to the increased incubation time which resulted in more cellular uptake of photosesitizers .