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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳瑞碧 | |
| dc.contributor.author | Hua-Ting Kung | en |
| dc.contributor.author | 宮華婷 | zh_TW |
| dc.date.accessioned | 2021-06-13T03:48:39Z | - |
| dc.date.available | 2011-07-31 | |
| dc.date.copyright | 2006-07-31 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-25 | |
| dc.identifier.citation | 林慶文。1989。乳品加工學。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32426 | - |
| dc.description.abstract | 初乳泛指哺乳類動物於生產後初期所分泌之乳汁。初乳與常乳在營養成分及免疫因子的組成上有很大的差別，例如初乳含有較高量之生長、免疫調節及抗菌因子等。嬰兒剛出生時，初乳能夠給予嬰兒適當的免疫防護，在幼體免疫系統發育成熟正常運作前，避免外在病原體之感染。本實驗採用第一天至第七天牛初乳，探討其對於人類周邊血液單核細胞生長之效果及經由間接模式對人類白血病細胞U937抑制之作用。結果發現，第一天與第二天初乳有促進人類單核細胞生長之能力，且在最佳濃度300 μg/mL下能有效的增加T細胞、Tc細胞與自然殺手細胞之比率。而在初乳對於人類白血病細胞U937生長抑制方面發現，唯有第一天到第四天初乳能有效的間接抑制U937細胞之生長，並誘導其分化為正常之人類單核球或巨噬細胞。另外，由初乳與單核細胞所共同培養之條件培養液中，亦可測得具有免疫調節作用之細胞激素IL-1β、IFN-γ及TNF-α。
乳鐵蛋白為初乳中最常被探討具有免疫活性之蛋白質，其在初乳中含量最高，由前人研究發現乳鐵蛋白含量隨著初乳天數增加而明顯降低。利用細胞實驗探討乳鐵蛋白在初乳免疫調節活性中所扮演的角色，實驗結果發現乳鐵蛋白能促進單核細胞的生長，但在間接抑制U937細胞生長方面並不明顯。此外，初乳以酸沉澱法初步分離的結果發現，含有乳鐵蛋白之沉澱物具有對於人類單核細胞生長之功效，而上清液則無效果。 | zh_TW |
| dc.description.abstract | Colostrum is breast milk produced after giving birth and lasts for 2-4 days. Bovine colostrum contains essential nutrients, growth factors, antimicrobial compounds and immune-regulating constituents either not present in milk or present in substantially lower concentrations. Colostrum is thought to protect neonates from infection, as well as to facilitate the immune maturation. In this study the immunomodulatory activities and anti-proliferation effect of human lukemic cell U937 were being invested. Results suggested that colostrum collected on day 1 and 2 after onset of lactation enhanced the growth of peripheral blood mononuclear cells (MNCs) in a dose dependent manner and significantly increased the population of T cell、Tc cell and NK cell. Moreover, day 1 to day 4 colostrums were capable to induce MNCs to secrete IL-1β, IFN-γ and TNF-α, which subsequently inhibited the growth of U937 cells and further induced it’s differentiation into mature monocytes or macrophages.
Lactoferrin is the most prominent immunomodulatory factor within colostrum. It has been proven that lactoferrin is at its maximum in the colostrum obtained at parturition and gradually decrease with time course of lactation. The present investigation demonstrated that lactoferrin, even at very low concentration, could enhance the growth of PBMC. However, its effect on growth inhibition of U937 was not significant. In addition, when colostrum was fractionated by acid precipitation, the MNC growth effect was found in the precipitated fraction containing lactoferrin. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T03:48:39Z (GMT). No. of bitstreams: 1 ntu-95-R93641016-1.pdf: 1080080 bytes, checksum: cccbd13aab776014bd8ac8d50859d9f8 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 中文摘要 I
Abstract II 目錄 III 表次 VI 圖次 VII 附錄次 X 壹、文獻回顧 1 1.1 初乳緒論 1 1.2 初乳之成分 2 1.2.1 酪蛋白 (Casein) 2 1.3 免疫系統 (Immune system) 11 1.3.1 先天或非專一性免疫防禦機制(Innate or non-specific immune response) 11 1.3.2 後天或專一性免疫防禦機制(Acquired or specific immune response) 12 1.3.3 淋巴類的細胞（Lymphoid cells） 13 1.3.4 細胞激素 (Cytokine) 16 1.3.5 人類白血病細胞株U937 20 1.3.6 流式細胞儀 (Flow cytometry) 21 貳、實驗目的與架構 23 2.1 實驗目的 23 2.1 實驗架構 24 2.1.1 實驗I：探討分娩後第一天到第七天之初乳經由間接模式對U937細胞之抑制作用以及對人類單核細胞之生長效果 25 2.1.2 實驗Ⅱ：探討HTST殺菌之初乳經由間接模式對U937細胞之抑制作用以及對人類單核細胞之生長效果 26 2.1.3 實驗III：探討乳鐵蛋白經由間接模式對U937細胞之抑制作用以及對人類單核細胞之生長效果 26 2.1.4 實驗IV：初乳之分離以及區分物免疫活性之探討 27 参、材料與方法 28 3.1 實驗材料 28 3.1.1 初乳 (Colostrum) 28 3.1.2 實驗細胞 28 3.1.3 實驗藥品 28 3.1.4 實驗器材 30 3.1.5 儀器設備 31 3.2 實驗方法 32 3.2.1 初乳經由間接模式對U937細胞抑制作用之實驗 32 3.2.2 評估U937細胞株之分化 - 細胞表面抗原CD11b及CD14之測定 36 3.2.3 樣品-單核細胞條件培養液細胞激素之含量測定 37 3.2.4 測定經由樣品刺激後人類單核細胞之生長比例 39 3.2.5 人類單核細胞表面抗原之分析 40 3.2.6 初乳HTST殺菌法 (High temperature short time pasteurization) 42 3.2.7 以酸沉澱方法分離初乳 42 3.2.8 以膜過濾方法分離初乳 43 3.2.9 以HPLC裝置GPC管柱分離初乳 44 3.2.10 蛋白質之定量 44 3.2.11 蛋白質膠體電泳法 45 3.2.12 統計分析 51 肆、實驗結果 52 4.1初乳經由間接模式對U937細胞之抑制作用以及對人類單核細胞之生長效果 52 4.1.1 初乳間接抑制U937細胞之實驗 52 4.1.2 U937細胞表面抗原CD11b及CD14之測定 53 4.1.3 分析條件培養液中細胞激素的含量 54 4.1.4 分析polymyxin B對細胞激素含量之影響 55 4.1.5 初乳促進人類單核細胞之生長效果 56 4.1.6 人類單核細胞表面抗原之分析 57 4.2 探討經HTST殺菌後之初乳經間接模式對U937細胞之抑制作用以及對人類單核細胞之生長效果 58 4.2.1 以間接模式探討HTST殺菌後之初乳對U937細胞之抑制作用 58 4.2.3 HTST殺菌後之初乳對人類單核細胞之生長效果 59 4.3 探討乳鐵蛋白經由間接模式對U937細胞之抑制作用以及對人類單核細胞之生長效果 60 4.3.1 以間接模式探討乳鐵蛋白對U937細胞之抑制作用 60 4.4 初乳之分離 61 4.4.1 酸沉澱分離法 61 4.4.2 膜過濾分離法 64 4.4.3 GPC分離法 65 伍、討論 67 5.1 初乳之免疫調節作用 67 5.1.1 分娩後第一天到第四天初乳經由間接模式抑制U937細胞生長 67 5.1.2 分娩後第一天到第二天初乳能促進單核細胞生長並提升T細胞、Tc細胞及自然殺手細胞之比率 69 5.2 探討HTST殺菌後之初乳 71 5.3 乳鐵蛋白提升人類單核細胞生長之趨勢 73 5.4 嘗試分離初乳成分 74 5.4.1 酸沉澱分離法 74 5.4.2 膜過濾分離法 75 5.4.3 GPC分離法 76 陸、結論 78 柒、參考文獻 79 | |
| dc.language.iso | zh-TW | |
| dc.title | 初乳之免疫調節作用及對人類白血病細胞(U937)之抑制效果 | zh_TW |
| dc.title | Colostrum’s Immunomodulatory Activities and Inhibitory Effects on Human Leukemic U937 Cells | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許輔,張基郁,江啟銘,廖慧芬 | |
| dc.subject.keyword | 初乳,人類白血病細胞, | zh_TW |
| dc.subject.keyword | Colostrum,Human Leukemic U937 Cells, | en |
| dc.relation.page | 126 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-26 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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