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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 孫璐西 | |
dc.contributor.author | Meng-Chieh Hsieh | en |
dc.contributor.author | 謝孟潔 | zh_TW |
dc.date.accessioned | 2021-06-13T03:46:57Z | - |
dc.date.available | 2016-07-26 | |
dc.date.copyright | 2006-08-01 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-26 | |
dc.identifier.citation | 行政院衛生署。2006。中華民國94年國人主要死因統計資料。
李沐勳和李威。2001。常用中草藥手冊。國立中國醫藥研究所。132。 沈宜蓁。2005。龍眼花萃取物抗氧化性之探討。國立台灣大學食品科技研究所碩士論文。 李時珍。明代。本草綱目。實用書局。1041。 洪鼎超。1997。蘇木,木棉花與百香果殼抗氧化成分之研究。東海大學食品科學研究所碩士論文。 郭靜娟。2001。薏苡籽實之抗氧化成分及其抑制自由基傷害之研究。國立台灣大學食品科技研究所碩士論文。 黃弼臣。1966。第四章 龍眼。農業要覽園藝作物輯果樹篇。台灣省政府農林廳。193-204。 秦玲。1988。龍眼單寧成分研究。台北醫學院藥學研究所碩士論文。 張景煇。1996。龍眼,龍眼乾,龍眼花及龍眼蜜中重要香味成份判定之研究。大葉工學院食品工程研究所碩士論文。 許鴻齡、李立聰。1977。龍眼花化學成分之研究。化學 4: 103-105. 樊謙騰。1996。鳳凰花及山竹果殼抗氧化成分與花青素之研究。東海大學食品科學研究所碩士論文。 簡永亮。1999。沙苑子、馬櫻丹花抗氧化成分及沙苑子揮發性成分之研究。東海大學食品科學研究所碩士論文。 Ali BH, Al Wabel N, Blunden G. 2005. Phytochemical, pharmacological and toxicological aspects of Hibiscus sabdariffa L.: a review. Phytother Res 19(5):369-75. Ames BN, Shigenage MK, Gold LS. 1993. DNA lesions, inducible DNA repair, and cell division: the three key factors in mutagenesis and carcinogenesis. Environ Health Perspect 101(suppl. 5):35-44. Arichi H, Kimura Y, Okuda H, Baba K, Kozawa M, Arichi S. 1982. Effects of stilbene components of the roots of polygonum on lipid metabolism. Chem Pharm Bull 30:1766–70. Aviram M, Dornfeld L, Kaplan M, Coleman R, Gaitini D, Nitecki S, Hofman A, Rosenblat M, Volkova N, Presser D, Attias J, Hayek T, Fuhrman B. 2002. Pomegranate juice flavonoids inhibit low-density lipoprotein oxidation and cardiovascular diseases: studies in atherosclerotic mice and in humans. Drugs Exp Clin Res 28(2-3):49-62. Bagchi D, Bagchi M, Stohs SJ, Das DK, Ray SD, Kuszynski CA, Joshi SS, Pruess HG. 2000. Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention. Toxicology 148(2-3):187-97. Bagchi D, Bagchi M, Stohs SJ, Ray SD, Sen CK, Preuss HG. 2002. Cellular protection with proanthocyanidins derived from grape seeds. Ann N Y Acad Sci 957:260-70. Bagchi D, Sen CK, Ray SD, Das DK, Bagchi M, Preuss HG, Vinson JA. 2003. Molecular mechanisms of cardioprotection by a novel grape seed proanthocyanidin extract. Mutat Res 523-524:87-97. Berliner JA, Heinecke JW. 1996. The role of oxidized lipoproteins in atherogenesis. Free Radic Biol Med 20(5):707-27. Bertelli AA, Giovannini L, Giannessi D. 1995. Antiplatelet activity of synthetic and natural resveratrol in red wine. Int J Tissue React 17:1–3. Borek C. 2004. Dietary antioxidants and human cancer. Integr Cancer Ther 3(4):333-41. Bors W, Heller W, Michel C, Saran M. 1990. Flavonoids as antioxidants: Determination of free radical-scavenging efficiencies. Methods Enzymol 186:343-55. Bravo L. 1998. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev 56(11):317-33. Cambell MK. 1999. Biochemistry, 3th edition. Orlando,FL:Harcourt Inc. p606-609. Cao G, Sofic E, Prior RL. 1997. Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radic Biol Med 22(5):749-60. Cao G, Prior RL. 1998. Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clin Chem 44, 1309-15. Chang YC, Huang KX, Huang AC, Ho YC, Wang CJ. 2006. Hibiscus anthocyanins-rich extract inhibited LDL oxidation and oxLDL-mediated macrophages apoptosis. Food Chem Toxicol 44(7):1015-23. Cheng JT, Lin TC, Hsu FL. 1995. Antihypertensive effect of corilagin in the rat. Can J Physiol Pharmacol 73:1425-29. Cuppett S, Schnepf M, Hall Ⅲ C. 1997. Natural antioxidants-Are they reality? In Shahidi F, editor. Natural Antioxidants: Chemistry, Health Effects, and Applications. Champaign, Illinois: AOCS Press. p12-24. Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. 2004. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India 52: 794-804. Diaz MN, Frei B, Vita JA, Keaney JF. 1997. Antioxidants and atherosclerotic heart disease. N Engl J Med 337(6):408-16. Espin JC, Soler-Rivas C, Wichers HJ. 2000. Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. J Agric Food Chem 48:648-56. Fine AM. 2000. Oligomeric proanthocyanidin complexes: history, structure, and phytopharmaceutical applications. Altern Med Rev 5(2):144-51. Foti MC, Daquino C, Geraci C. 2004. Electron-transfer reaction of cinnamic acids and their methyl esters with the DPPH free radical in alcoholic solutions. J Org Chem 2004 69(7):2309-14. Frankel EN, Waterhouse AL, Kinsella JE. 1993. Inhibition of human LDL oxidation by resveratrol. Lancet 341:1103–4. Fuhrman B, Aviram M. 2001. Flavonoids protect LDL from oxidation and attenuate atherosclerosis. Curr Opin Lipidol 12(1):41-8. Guo C, Yang J, Li Y, Xu J, Jiang Y. 2003. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr Res 23: 1719-26. Gutierrez ME, Garcia AF, Africa de Madariaga M, Sagrista ML, Casado FJ, Mora M. 2003. Interaction of tocopherols and phenolic compounds with membrane lipid components: evaluation of their antioxidant activity in a liposomal model system. Life Sci 72(21):2337-60. Hart H, Craine LE, Hart DJ. 1999. Spectroscopy and structure determination. In: Organic chemistry 10th edition. Boston, MA: Houghtion Mifflin Company. p348-381. Hirano R, Sasamoto W, Matsumoto A, Itakura H, Igarashi O, Kondo K. 2001. Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation. J Nutr Sci Vitaminol (Tokyo) 47(5):357-62. Halliwell B and Gutteridge JMC. 1999a. Antioxidant Defences. In Free radicals in Biology and Medicine, 3rd Ed. Oxford University Press. New York, U.S.A. Hirunpanich V, Utaipat A, Morales NP, Bunyapraphatsara N, Sato H, Herunsale A, Suthisisang C. 2006. Hypocholesterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa L. in hypercholesterolemic rats. J Ethnopharmacol 103(2):252-60. Ho KY, Huang JS, Tsai CC, Lin TC, Hsu YF, Lin CC. 1999. Antioxidant activity of tannin components from Vaccinium Vitis-idaea L. J Pharm Pharmacol 51: 1075-78. Hsu FL, Lu FH, Cheng JT. 1994. Influence of acetonylgeraniin, a hydrolyzable tannin from Euphoria longana, on orthostatic hypotension in a rat model. Planta Med 60: 297-300. Huang D, Ou B, Prior RL. 2005. The chemistry behind antioxidant capacity assays. J Agric Food Chem 53(6):1841-56. Huang TH, Peng G, Kota BP, Li GQ, Yamahara J, Roufogalis BD, Li Y. 2005. Anti-diabetic action of Punica granatum flower extract: activation of PPAR-gamma and identification of an active component. Toxicol Appl Pharmacol 207(2):160-9. Jacques D, Haslam E, Bedford GR, Greatbanks D. 1974, Plant proanthocyanidins. Part II. Proanthocyanidin-A2 and its derivatives J Chem Soc Perkin Trans 1. 2663-71. Jiang F, Dusting GJ. 2003. Natural phenolic compounds as cardiovascular therapeutics: potential role of their antiinflammatory effects. Curr Vasc Pharmacol 1(2):135-56. Julkunen-Tiitto R. 1985. Phenolic constituents in the leaves of northern willows: methods for analysis of certain phenolics. J Agric Food Chem 33: 213-7. Kaliora AC, Dedoussis GV, Schmidt H. 2005. Dietary antioxidants in preventing atherogenesis. Atherosclerosis 187(1):1-17. Kandaswami C, Middleton E. 1997. Flavonoid as antioxidant. In Shahidi F, editor. Natural Antioxidants: Chemistry, Health Effects, and Applications. Champaign, Illinois: AOCS Press. p258-270. Karakaya S. 2004. Bioavailability of phenolic compounds. Crit Rev Food Sci Nutr 44:453-64. Kaur G, Jabbar Z, Athar M, Alam MS. 2006. Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food Chem Toxicol 44(7):984-93. Kim HJ, Lee YS. 2005. Identification of new dicaffeoylquinic acids from Chrysanthemum morifolium and their antioxidant activities. Planta Med 71(9):871-6. Kris-Etherton PM, Keen CL. 2002. Evidence that the antioxidant flavonoids in tea and cocoa are beneficial for cardiovascular health. Curr Opin Lipidol 13:41-49. Labieniec M, Gabryelak T. 2006. Interactions of tannic acid and its derivatives (ellagic and gallic acid) with calf thymus DNA and bovine serum albumin using spectroscopic method. J Photochem Photobiol B 82(1):72-8. Latte KP, Kolodziej H. 2000. Antifungal effects of hydrolysable tannins and related compounds on dermatophytes, mould fungi and yeasts. Z Naturforsch 55c:467-472. Leung LK, Su Y, Chen R, Zhang Z, Huang Y, Chen ZY. 2001. Theaflavins in black tea and catechins in green tea are equally effective antioxidants. J Nutr 131(9):2248-51. Lin JH, Tsai CC. 1995. Phenolic constituents from the flowers of Euphoria longana Lam. Chin Pharm J 47: 113-121. Liu RH. 2004. Potential synergy oh phytochemicals in cancer prevention: Mechanism of action. J Nutr 134(12 Suppl):3479S-3485S. Lo AH, Liang YC, Lin-Shiau SY, Ho CT, Lin JK. 2002. Carnosol, an antioxidant in rosemary, suppresses inducible nitric oxide synthase through down-regulating nuclear factor-kappaB in mouse macrophages. Carcinogenesis 23(6):983-91. Loo B, Bachschmid M, Spitzer V, Brey L, Ullrich V, and Luscher TF. 2003. Decreased plasma and tissue levels of vitamin C in a rat model of aging: Implications for antioxidant defense. Biochem and Biophys Res Comm 303:483–87. Lopez-Velez M, Martinez-Martinez F, Del Valle-Ribes C. 2003. The study of phenolic compounds as natural antioxidants in wine. Crit Rev Food Sci Nutr 43(3):233-44. Lou H, Yamazaki Y, Sasaki T, Uchida M, Tanaka H, Oka S. 1999. A-type proanthocyanidins from peanut skins. Phytochemistry 51(2):297-308. Maatta-Riihinen KR, Kahkonen MP, Torronen AR, Heinonen IM. 2005. Catechins and procyanidins in berries of vaccinium species and their antioxidant activity. J Agric Food Chem. 53(22):8485-91. Minakata H, Komura H, Tamura SY, Ohfune Y, Nakanishi K, Kada T. 1985. Antimutagenic unusual amino acids from plants. 1622-1623. Nakatani N. 1997. Antioxidanys from spices and herbs. 1997. In Shahidi F, editor. Natural Antioxidants: Chemistry, Health Effects, and Applications. Champaign, Illinois: AOCS Press. p64-75. Natella F, Belelli F, Gentili V, Ursini F, Scaccini C. 2002. Grape seed proanthocyanidins prevent plasma postprandial oxidative stress in humans. J Agric Food Chem 50:7720-5. Nilsson J, Regnstrom J, Frostegard J, Stiko A. 1992. Lipid oxidation and atherosclerosis. Herz 17(5):263-9. Okabe S, Suganuma M, Imayoshi Y, Taniguchi S, Yoshida T, Fujiki H. 2001. New TNF-R releasing inhibitors, geraniin and corilagin, in leaves of Acer nikoense, Megusurino-ki. Biol Pharm Bull 24:1145-8. Okuyama E, Ebihara H, Takeuchi H, Yamazaki M. 1999. Adenosine, the anxiolytic-like principle of the arillus of Euphoria longana. Planta Med 65: 115-9. Ou B, Hampsch-Woodill M, Prior RL. 2001. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem 49:4619-26. Park KY, Jung GO, Choi J, Lee KT, Park HJ. 2002. Potent antimutagenic and their anti-lipid peroxidative effect of kaikasaponin III and tectorigenin from the flower of Pueraria thunbergiana. Arch Pharm Res 25(3):320-4. Parthasarathy S, Rankin SM. 1992. The role of oxidized LDL in atherogenesis. Prog Lipid Rex 31: 127- 43. Pietta PG. 2000. Flavonoids as antioxidants. J Nat Prod 63(7):1035-42. Pratico D, Delanty N. 2000. Oxidative injury in diseases of the central nervous system: Focus on Alzheimer’s Disease. Am J Med 109:577–585. Prior RL, Cao GH. 2000. Analysis of botanicals and dietary supplements for antioxidant capacity: A review. J. AOAC Int 83 (4):950-6. Prior RL, Hoang H, Gu L, Wu X, Bacchiocca M, Howard L, Hampsch-Woodill M, Huang D, Ou B, Jacob R. 2003. Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORACFL)) of plasma and other biological and food samples. J Agric Food Chem 51:3273-9. Prior RL, Wu X, Schaich K. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53(10):4290-302. Porter LJ, Newmen RH, Foo LY, Wong H. 1982. Polymeric proanthocyanidins. 13C N.M.R. studies of procyanidins. J Chem Soc Perkin Trans 1. 1217-21. Puhl H, Waeg G, Esterbauer H. 1994. Methods to determine oxidation of low-density lipoproteins. Methods Enzymol 233:425-41. Rangkadilok N, Worasuttayangkurn L, Bennett RN, Satayavivad J. 2005. Identification and quantification of polyphenolic compounds in Longan (Euphoria longana Lam.) fruit. J Agric Food Chem 53(5):1387-92. Reed J. 2002. Cranberry flavonoids, atherosclerosis and cardiovascular health. Crit Rev Food Sci Nutr 42(3 Suppl):301-16. Remacle J, Lambert D, Raes M, Pigeolet E, Michiels C and Toussaint O. 1992. Importance of various antioxidant enzymes for cell stability. Confrontation between theoretical and experimental data. Biochem J 286:41-6. Richomond W. 1973. Preparation and properties of a cholesterol oxidase from Nocardia sp. And it’s application to the enzymatic assay of total cholesterol in serum. Cline Chem 19:1350-6 Rotondo S, Rajtar G, Manarinis S. 1998. Effect of trans-resveratrol, a natural polyphenolic compound, on human polymorphonuclear leukocyte function. Br J Pharmacol 123:1691–9. Rudel LL, Sawyer JK, Parks JA. 1991. Dietary fat, Lipoprotein structure, and atherosclerosis in primates. In: Weber PC and Leaf A, editors. Atherosclerosis : its pathogenesis and the role of cholesterol, Atherosclerosis reviews v. 23. New York : Raven Press. P41-50. Shahidi F. 1996. Natural antioxidants: An overview. 1997. In Shahidi F, editor. Natural Antioxidants: Chemistry, Health Effects, and Applications. Champaign, Illinois: AOCS Press. p1-11. Shahidi F. 2000. Antioxidants in food and food antioxidants. Nahrung 44(3):158-63. Shimada K, Fujikawa K, Yahara K, Nakamura T. 1992. Antioxidative properties of Xanthan on the autoxidative of soybean oil in cyclodextrin emulsion. J Agric Food Chem 40:945-8. Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299: 152-78. Sotelo-Felix JI, Martinez-Fong D, Muriel P, Santillan RL, Castillo D, Yahuaca P. 2002. Evaluation of the effectiveness of Rosmarinus officinalis (Lamiaceae) in the alleviation of carbon tetrachloride-induced acute hepatotoxicity in the rat. J Ethnopharmacol 81(2):145-54. Stangl V, Lorenz M, Stangl K. 2006. The role of tea and tea flavonoids in cardiovascular health. Mol Nutr Food Res 50(2):218-28. Stocker R, Keaney JF. 2004. Role of oxidative modification in atherosclerosis. Physiol Rev 84:1381-478. Tsao R, Deng Z. 2004. Separation procedures for naturally occurring antioxidant phytochemicals. J Chromatogr B Analyt Technol Biomed Life Sci 812(1-2):85-99. Tucker JM, Townsend DM. 2005. Alpha-tocopherol: roles in prevention and therapy of human disease. Biomed Pharmacother 59(7):380-7. Willcox JK, Ash SL, Catignani GL. 2004. Antioxidants and prevention of chronic disease . Crit Rev Food Sci Nutr 44(4):275-95. Wong KC. 2000. Longan production in Asia. Food and agriculture organization of the united nations. Bangkok, Thailand. Yuan YV, Kitts DD. 1997. Endogenous antioxidants: Role of antioxidant enzymes in biological Systems. In Shahidi F, editor. Natural Antioxidants: Chemistry, Health Effects, and Applications. Champaign, Illinois: AOCS Press. p258-270. Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555-9. Zhu QY, Huang Y, Tsang D, Chen ZY. 1999. Regeneration of alpha-tocopherol in human low-density lipoprotein by green tea catechin. J Agric Food Chem. 47(5):2020-5. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32397 | - |
dc.description.abstract | 動脈粥狀硬化為造成心血管疾病之主要原因,而低密度脂蛋白(low density lipoprotein, LDL)膽固醇的氧化為啟動動脈粥狀硬化的重要步驟;抗氧化劑可以增加LDL對氧化傷害的抵抗力,因此攝取含有抗氧化成分之食品應有助於預防動脈粥狀硬化之發生。本研究室於前一年度研究顯示,龍眼(Dimocarpus longan Lour.)花具有良好之抗氧化效果,因此本研究以抗氧化活性為導向進行龍眼花活性成分之分離純化,並以「抑制銅離子誘導人類LDL之氧化」為主要抗氧化活性之檢測方法,進而鑑定出有效之抗氧化成分。
龍眼花甲醇萃取物以正己烷、乙酸乙酯、正丁醇及水四種溶劑進行分配萃取,其中以乙酸乙酯區分層之抗氧化效果最佳,其對於清除DPPH自由基之EC50為5.28 ± 1.14 μg/mL。在延緩LDL氧化方面,其效果為同濃度(1 μg/mL)下Trolox之1.14倍,並且具有最高之總酚(701.7 ± 29.3 mg gallic acid /g dry weight of sample)與總類黃酮(213.7 ± 14.8 mg catechin equivalent /g dry weight of sample)含量。 進一步將乙酸乙酯區分層以矽膠管柱層析法予以分離純化,得到20個次區分。由DPPH自由基清除能力試驗可知,中至高極性之次區分(F9~19)具有較好之抗氧化效果。ORAC試驗結果顯示F8~11之抗氧化效果十分優異,其中以F9(以ethyl acetate/n-hexane=60/40, v/v沖提)最佳,ORAC值為25.07 ± 4.08 Trolox equivalent。抑制銅離子誘導人類LDL氧化試驗結果亦顯示,F8~11可有效延緩LDL之氧化,其中以F10(以ethyl acetate/n-hexane=70/30, v/v沖提)之效果特別突出,為同濃度(1 μg/mL)下Trolox之1.72倍。分析各次區分之抗氧化物質含量,發現F9之總酚(970.4 ± 11.2 mg gallic acid /g dry weight of sample)與總類黃酮(732.9 ± 19.0 mg catechin /g dry weight of sample )含量皆為最高;且F8~11之總類黃酮含量趨勢與抗氧化效果之趨勢相符,並皆顯著高於區分前之乙酸乙酯區分層。因此推測龍眼花之抗氧化性可能與酚類中類黃酮物質含量或組成具有高度相關性。 HPLC分析F9發現其僅含一個成分,該化合物以 IR、MS、UV-Vis與1H-NMR、13C-NMR、2D-NMR等光譜分析,鑑定出F9中主要抗氧化活性成分為(-)-epicatechin。以Sephadex LH-20分離F10中成分後,則鑑定出其中兩個主要抗氧化活性成分為(-)-epicatechin與proanthocyanidin A2,兩者皆具抑制LDL氧化效果,分別為同濃度(0.5 μg/mL)下Trolox之1.95與2.04倍。每克龍眼花中(-)-epicatechin與proanthocyanidin A2之含量經HPLC分析,各約為5.58 mg及1.70 mg。本實驗結果顯示龍眼花中具有優異抗氧化效果之成分,有潛力發展為具有保健功效之食材。 | zh_TW |
dc.description.abstract | Atherosclerosis is the major cause of cardiovascular disease, and the oxidation of low density lipoprotein (LDL) cholesterol is the important step to initiate atherosclerosis. Antioxidants can increase the resistance against oxidative damage, so the supplementation of food with antioxidants may help prevent the incidence of atherosclerosis. Previous year study in our laboratory has shown that Longan (Dimocarpus longan Lour.) flower had good antioxidative activity. Therefore, the objective of this study is to conduct antioxidant activity-guided separation and purification of Longan flower by the major antioxidative assay, the inhibition of Cu2+-induced human LDL oxidation, and to identify the effective compounds.
After liquid-liquid partition of Longan flower methanol extract with n-hexane, ethyl acetate, n-butanol and water, the ethyl acetate fraction showed the best antioxidant activity. The EC50 value of the ethyl acetate fraction in scavenging DPPH radicals was 5.28 ± 1.14 μg/mL, and its effect of delaying LDL oxidation is 1.14 times better than Trolox at the same concentration level (1μg/mL). Besides, the ethyl acetate fraction had the highest contents of total polyphenol (701.7 ± 29.3 mg gallic acid /g dry weight of sample) and flavonoid (213.7 ± 14.8 mg catechin equivalent /g dry weight of sample). Silica gel chromatography was employed to fractionate the ethyl acetate fraction of Longan flower methanol extract, and twenty sub-fractions were obtained. DPPH assay showed that the sub-fractions with medium to high polarity had better antioxidative activities. Result of ORAC assay revealed that F8~11 were the more effective sub-fractions and F9 (eluted by ethyl acetate/n-hexane=60/40, v/v) gave the highest ORAC value (25.07 ± 4.08 Trolox equivalent). As for the effect of Cu2+-induced oxidation of human LDL, F8~11 also showed better effect in delaying LDL oxidation. Among them, F10 (eluted by ethyl acetate/n-hexane=70/30, v/v), which had superior effect, was 1.72 times better than Trolox at the same concentration level (1μg/mL). Further analysis of these sub-fractions showed that F9 contained the hightest amounts of total polyphenol (970.4 ± 11.2 mg gallic acid /g dry weight of sample) and total flavonoid (732.9 ± 19.0 mg catechin /g dry weight of sample). The trends of total flavonoid contents and the antioxidative activities of the four sub-fractions (F8~11) were similar, we therefore supposed that the antioxidative activity of Longan flower was highly related to its total flavonoid content or composition. There was only one major compound present in F9 by HPLC analysis and it was identified as (-)-epicatechin by spectrometric analysis of IR, MS, UV-Vis, 1H-NMR, 13C-NMR and 2D-NMR. After separating F10 by Sephadex LH-20, two major components were identified as (-)-epicatechin and proanthocyanidin A2. Both of them had superior effect in delaying LDL oxidation, and the lag time of each compound was 1.95 ( (-)-epicatechin ) and 2.04 ( proanthocyanidin A2 ) times better than Trolox at the same concentration level (0.5μg/mL). The contents of (-)-epicatechin and proanthocyanidin A2 in Longan flower were quantified by HPLC to be 5.58 and 1.70 mg/g dry weight, respectively. This study showed that Longan flower contained components with excellent activity, it thus has good potential to be developed as a functional food. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T03:46:57Z (GMT). No. of bitstreams: 1 ntu-95-R93641007-1.pdf: 7438989 bytes, checksum: a7bcf14eda0917ca2a2c315898686802 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 目錄
摘要 Ⅰ 英文摘要 Ⅲ 目錄 Ⅴ 表次 Ⅷ 圖次 Ⅸ 壹、 前言 1 貳、 文獻整理 2 一、 自由基與氧化傷害 2 二、 氧化壓力與疾病 4 1. 癌症 4 2. 心血管疾病 5 3. 阿茲海默症與老化 5 三、 脂蛋白及動脈粥狀硬化 6 1. 脂蛋白 6 2. 低密度脂蛋白 7 3. 低密度脂蛋白的氧化 7 4. 動脈粥狀硬化 9 5. 抗氧化劑與動脈粥狀硬化之關聯 11 四、 抗氧化物質的防禦 12 1. 內源性抗氧化物質 14 2. 飲食中提供的抗氧化物質 17 (1) 維生素C 17 (2) 維生素E 17 (3) 類胡蘿蔔素 18 (4) Coenzyme Q10 18 (5) 多酚類化合物 18 a. 類黃酮 19 b. 酚酸 21 c. 原花青素 23 五、 天然食材中之抗氧化物質 25 1. 茶 25 2. 葡萄與紅酒 27 3. 香辛料與草本植物 28 4. 花 30 六、 龍眼花 33 1. 植物概況 33 2. 龍眼成分與功效 34 3. 龍眼花成分與功效 35 參、 研究目的與實驗架構 38 一、 研究目的 38 二、 實驗架構 39 肆、 材料與方法 42 一、 實驗材料 42 二、 化學藥品與試劑 42 (一) 化學藥品 42 (二) 溶劑 43 (三) 酵素套組與電泳膠片 44 (四) 層析材料 44 三、 儀器設備 45 (一) 樣品前處理相關儀器設備 45 (二) 化學分析相關儀器設備 45 (三) 結構鑑定相關儀器設備 46 四、 實驗方法 47 (一) 龍眼花中水分之含量分析 47 (二) 甲醇萃取物之製備 47 (三) 甲醇萃取物之溶劑分配萃取 47 1. 小量製備 47 2. 大量製備 48 (四) 抗氧化性檢測方法 48 1. DPPH自由基清除能力之測定 48 2. 抑制銅離子誘導人類LDL氧化試驗 49 3. 氧自由基吸收能力之測定(ORAC) 52 (五) 抗氧化成分分析 53 1. 總酚類化合物 (Total polyphenol) 含量之測定 53 2. 總類黃酮 (Total flavonoid) 含量之測定 53 (六) Silica gel管柱層析 54 1. 小量管柱層析(供抗氧化活性測試用) 54 2. 大量製備(製備活性次區分) 55 (七) 薄層層析法 56 (八) Sephadex LH-20管柱層析 57 (九) 高效能液相層析分析 57 (十) 化合物的結構鑑定與分析 58 五、 統計分析 59 伍、 結果與討論 60 一、 龍眼花之甲醇萃取 60 二、 龍眼花甲醇萃取物之溶劑分配萃取 60 三、 龍眼花甲醇萃取物不同溶劑分配萃取區分層之抗氧化性試 驗與分析 61 1. DPPH自由基清除能力試驗 61 2. 抑制銅離子誘導人類LDL氧化試驗 65 3. 總酚與總類黃酮含量分析 70 四、 龍眼花甲醇萃取物乙酸乙酯區分層之矽膠管柱層析 73 五、 龍眼花甲醇萃取物乙酸乙酯區分層經矽膠管柱層析後各次 區分之抗氧化性試驗與分析 73 1. DPPH自由基清除能力試驗 73 2. 氧自由基吸收能力(ORAC)測試 77 3. 抑制銅離子誘導人類LDL氧化試驗 81 4. 總酚與總類黃酮含量分析 84 六、 高效能液相層析法之分析 87 七、 龍眼花M-EA次區分之大量製備以及抗氧化試驗與分析結 果 87 八、 次區分F10’之Sephadex LH-20管柱層析 89 九、 純化物P1與P2之抑制銅離子誘導人類LDL氧化試驗 89 十、 純化合物之結構分析與鑑定 93 1. F9與P1之鑑定 94 2. P2之鑑定 103 十一、 兩種主要抗氧化成分之定量分析 112 陸、 結論 115 柒、 參考文獻 116 捌、 附錄 123 | |
dc.language.iso | zh-TW | |
dc.title | 龍眼花抗氧化成分之研究 | zh_TW |
dc.title | Studies on the Antioxidative Components of
Longan (Dimocarpus longan Lour.) Flower | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林哲輝,蘇正德,郭悅雄,江文章 | |
dc.subject.keyword | 抗氧化,龍眼花,epicatechin,proanthocyanidin A2, | zh_TW |
dc.subject.keyword | antioxidant,Longan flower,epicatechin,proanthocyanidin A2, | en |
dc.relation.page | 134 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-26 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
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