預防齲齒含氟貼片的研發

Abstract

摘要

在影響口腔健康的眾多原因之中，齲齒佔有極重要的地位；雖然目前市面上用於預防齲齒之含氟製品琳瑯滿目，然而台灣地區學童罹患齲齒之情形卻日益嚴重，因此，本研究將研發預防齲齒之含氟貼片，以提供一個具有良好成效且在使用上更加便利的齲齒預防方法 製備含氟貼片之溶液以氟化鈉(NaF)及增黏劑聚丙烯酸(carbopol)為主要成分，而經由實驗發現，40000 ppm NaF及3g carbopol/100ml H2O可使貼片之氟化物具有良好之質傳效率，同時其黏度亦足夠提供貼片所需之貼附力。本實驗利用可調式刮刀，將自行配製的含氟溶液於保鮮膜上刮出約500μm厚之薄膜，並加以裁剪製成含氟貼片。 在體外實驗中(in vitro)，以SEM/EDX測量牙齒標本在不同的貼附時間後，吸附於牙釉質表面的氟濃度，而由牙表面之氟濃度曲線可知，經過約8小時的貼片貼附，牙釉質表面之氟濃度可達到飽和；同時藉由鈣離子電極的檢測可發現，釋氟貼片在每四天使用一次，每次使用4小時，且持續使用2週後，對於牙齒標本抑制去礦化之能力可獲得顯著的提升；而針對具有初期齲齒之牙齒，貼片的使用可促進唾液(再礦化溶液)中鈣離子的沉積，且由本實驗證實，經過4小時的單次貼附並於再礦化溶液進行3天的浸泡，便可觀察到良好的再礦化效果。

Abstract

Dental caries plays a very important role on the health of the oral cavity, and it is one of the main reasons causing buccal diseases. Although there are various fluoride-containing commodities on the market, the prevalence of dental caries in Taiwan is always at a high level. Thus, we hope that research and development of fluoride strips can provide a more convenient and safe way to decrease the occurrence of dental caries. Sodium fluoride and carbopol(poly acrylic acid) are the most important components in the gel used to make fluoride-containing strips. We found that 40000 ppm NaF and 3g carbopol/100ml H2O provide appropriate viscosity for attaching to teeth and exhibit good diffusion rate of fluoride. We made fluoride-containing strips by spreading 500μm-thickness film on the PE membrane with the scraper and cut it into small size according to what we need. We measure fluoride concentration on the enamel surface by using SEM/EDX, and find that the fluoride adsorbed on the teeth achieves to the maximum after 8-hours sticking times. Then, we analyze effects of the fluoride-containing strips on demineralization-inhibition and remineralization-promotion by using the calcium electrode. We can know that fluoride strips can show excellent efficacy on demineralization-inhibition of dental enamel after 2 weeks by using it 4 hours a time, and two times a week. Besides, the ability of fluoride strips for remineralization-promotion of dental enamel is also confirmed after 4-hours sticking time and soaking teeth in remineralizing solution for 3 days. Thus, we can conclude that fluoride-containing strips have remarkable effects on prophylaxis of dental caries by in vitro study, and the advantage of convenience and safety makes it possess developmental potential.