電紡實驗在韌帶組織工程上的應用

Abstract

韌帶損傷常發生於許多運動及活動中，現在的治療方法不外乎自體移植或人工韌帶，但是病人在自體移植時要承受多餘的痛苦，而人工韌帶的製成尚未達到理想的標準，所以還有很大的進步空間。 電紡織的技術，早就在近七十年前就已經有專利技術了，由於可以克服表面張力而噴射出有奈米等級的纖維絲，在許多組織工程的研究中都漸漸受到重視。幾丁聚醣是已經被FDA認證的聚合物，在臨床上的應用十分廣泛，加上此材料不僅是價格便宜，更有良好的生物相容性、生物可分解性和低生物毒性，是十分理想的支架材料。 這篇文章利用幾丁聚醣為主要材料，以電紡織的技術製作有奈米等級的纖維，由於電紡織所要調控的參數很多，如：溶質和溶劑的選擇、電場的大小、電紡速度、噴嘴的選擇和適當的選擇添加物等，每一細微變化可能都會影響產物的狀態和性質，所以要不斷嘗試不同的參數，使所得的奈米纖維絲可以趨於理想。 在得到理想參數之後，利用高速滾輪將奈米纖維絲做有規律地收集，以做成奈米等級的基質，並經中和處理來增加細胞的貼附能力，最後培養上韌帶細胞加以觀察，希望能夠發展出一個理想的韌帶組織工程材料。

Ligamentary injury usually occurs in many sports or activities. Therapeutic treatments included autograft and artificial ligament reconstruction. Due to the reasons that patients would suffer a lot from autograft, and the artificial ligament did not reach the best standard, there is still some space to improve. Although the electrospinning technique has been patented for seventy years, people have paid more and more attention in the field of tissue engineering due to the ability of making nanofiber. Chitoson, a polymer that has been proved by FDA and been applied in clinical use, it is a suitable supporting material not only due to its inexpensive price, but also its great biocompatibility, biodegradability, and low bio-toxicity. In this article, we use chitoson as the main material to generate nanofiber by electrospinning. There are many parameters in electrospinning, such as the choice of solute and solvent, the size of the electric field, the velocity of electrospinning, the choice of jet and proper addictive. Every subtle change can affect the condition and property of the products; therefore, we have to try different parameters to make the nanofiber more ideal. After getting the ideal parameter, we use high speed rotor regularly collect the nanofiber. This nanofiber was neutralized to increase the ability of cell attachment. We wish to develop an ideal material for ligament tissue engineering by observing the condition of nanofiber which cultured with ligament cell.