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標題: | 具離子電泳功能並可長期植入之神經探針 Design and fabrication of neuroprobes for chronic recording and iontophoresis |
作者: | Chu-Lin Fan 范矩霖 |
指導教授: | 趙福杉(Fu-Shan Jaw) |
共同指導教授: | 楊龍杰(Lung-Jieh Yang) |
關鍵字: | 微機電,神經探針,微流道,生物相容性,聚-對二甲苯, MEMS,neuroprobe,microfluidic channel,biocompatibility,parylene, |
出版年 : | 2007 |
學位: | 碩士 |
摘要: | 在現代的電生理的研究中,常使用神經探針以記錄腦神經電位。本研究使用微機電系統技術,設計與製造新型神經探針,依據其功能的不同,本研究的神經探針可分為兩種:完全以聚-對二甲苯包覆之神經探針與具有微流道之神經探針。
對長期植入生物體內的微元件而言,生物相容性的考量是不可或缺的一項指標,為了讓以矽為基材的神經探針更適合進行長期植入的實驗,本研究成功以具有高度生物相容性的高分子材料:「聚-對二甲苯」將探針完全包覆,本研究發展創新的微元件全包覆技術,將微元件整體包覆聚-對二甲苯並且可允許特定位置開洞,突破微機電製程技術以往僅能進行微元件單面的包覆處理。 將神經探針整合微流道,使得探針不但可以傳輸微流體以刺激生物組織,還可以觀察組織受刺激前後的神經電位變化。本研究整合二條微流道於一神經探針上,相對於之前文獻上的相關的研究,本研究省去利用外接的注射幫浦驅動微流體,而改以在微流道前後端佈置電極,提供電壓下,可在微流道中產生離子電泳以驅動微流道內帶電之試劑,。 本研究成功量測兩種神經探針的電氣規格。並以完全聚-對二甲苯包覆之神經探針植入大鼠的大腦皮質中進行動物實驗,可記錄到大鼠後腳受機械刺激所誘發之大腦神經訊號。具有微流道之神經探針上經酒精與純水測試後,確定其微流道(長7釐米、寬60微米)中無任何阻塞。 Nowadays, neuroprobes are commonly used in electrophysiological studies. Novel neuroprobes are designed and implemented with Micro-Electro-Mechanical-System technology in this thesis. There are two kinds of neuroprobes designed: one is the completely parylene-coated and the other is that with micro-fluidic channels neuroprobes. Biocompatibility is essential for a device to be implanted in a living organism chronically. In order to make the silicon-based probes serve this purpose, the designed neuroprobes were completely coated with parylene a high polymer biocompatible material. The neuroprobe integrated with micro-fluidic channel can not only deliver agents to stimulate the tissues but also record the evoked neural potentials. For each neuroprobe two micro-fluidic channels were integrated. Electrodes placed at the front and the end of micro-fluidic channel were applied with a voltage that can cause iontophoresis to drive the agents. We have measured the electrical specifications of two neuroprobes. Completely parylene-coated neuroprobe was implanted in the primary sensory cortex of a living rat and mechanical stimuli were applied on the contralateral hind paw. The evoked potentials was recorded successfully. We tested micro-fluidic channel neuroprobe both by alcohol and by water, and demonstrated that the micro-fluidic channels (7mm-long and 60µm-wide) could function correctly. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30664 |
全文授權: | 有償授權 |
顯示於系所單位: | 醫學工程學研究所 |
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