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標題: | 聚乙二醇交聯幾丁聚醣作為創傷敷料之應用研究 Crosslinking chitosan with PEG for wound dressing development study |
作者: | Yi-Ting Lai 賴奕廷 |
指導教授: | 謝國煌(Kuo-Huang Hsieh) |
關鍵字: | 幾丁聚醣,聚乙二醇,交聯,水膠,創傷敷料, chitosan,PEG,cross-link,hydrogel,wound dressing, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | 幾丁聚醣其具有良好的生物性質,例如生物相容性、生物降解性以及抗菌性,且幾丁聚醣對於血液有促進凝結止血的效果。然而幾丁聚醣的低機械性質限制其應用範圍。幾丁聚醣在酸性條件下會形成具有帶正電胺基(amine group)的聚電解質,可和帶負電的聚電解質或生長因子形成錯合物,或是和具有羧基(carboxylic acid)的物質藉由醯胺鍵(amide bond)形成交聯結構,以修飾(modify)幾丁聚醣材料的性質。
本研究選用不同分子量和比例的聚乙二醇交聯幾丁聚醣,探討其物理化學性質和生物性質。除此之外,在動物實驗方面以全層皮膚缺損作為模型發現聚乙二醇交聯幾丁聚醣薄膜有助於Male Balb/c小鼠傷口癒合。從病理組織切片觀察,幾丁聚醣敷料有刺激纖維母細胞的增生能力和抑制發炎細胞的作用,而聚乙二醇添加,對於小鼠背部上皮細胞轉移有增進的效果。 綜合以上,聚乙二醇交聯幾丁聚醣是極具潛力的創傷敷料。 Chitosan has outstanding biological properties, including biocompatible, biodegradable and antibacterial ability. Moreover, chitosan has hemostatic ability for promoting blood to coagulate together. However, the applications of chitosan are limited for its low mechanical properties. Chitosan existing in acid environment becomes a cationic polyelectrolyte due to its amine group, it can easily form polyelectrolyte complexes with other anionic polyelectrolytes and growth factors, or form crosslink structure with carboxylic group by composing amide bond. Through this strategy, the properties of chitosan can be modified and enhanced. In this research, we studied the properties of materials made by cross-linked chitosan with different poly(ethylene glycol)(PEG) molecular weight and various PEG to chitosan ratio. The physicochemical characterization and biological properties were studied for the PEG crosslinked chitosan films. Furthermore, an in vivo animal test using Male Balb/c mice showed rather good wound healing effect of PEG crosslinked chitosan film in the full-thickness skin defect model. From the histological examination, chitosan could suppress the infiltration of inflammatory cells and accelerate fibroblast proliferation while the PEG could enhance epithelial migration. Thus, PEG crosslinked chitosan films can be a potential wound dressing with excellent forming and enhanced wound healing. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30408 |
全文授權: | 有償授權 |
顯示於系所單位: | 化學工程學系 |
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