請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30408
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝國煌(Kuo-Huang Hsieh) | |
dc.contributor.author | Yi-Ting Lai | en |
dc.contributor.author | 賴奕廷 | zh_TW |
dc.date.accessioned | 2021-06-13T02:03:07Z | - |
dc.date.available | 2014-08-04 | |
dc.date.copyright | 2011-08-04 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-02 | |
dc.identifier.citation | 1. Langer, R.V.J., Tissue engineering. Science, 1993. 260: p. 920-6.
2. 吳豐智, 曾如鈴, 神奇的物質-幾丁質和幾丁聚醣. 化工技術, 1997. 5: p. 196-201. 3. Madhavan, P., Chitin, Chitosan and their Novel Applications, in Science Lectrue Series. 1992: Kochi. p. 1. 4. Muzzarelli, R.A.A., Natural Chelating Polymer, in Pergamon Press. 1973: New York. p. 83. 5. Zikakis, J.P., Chitin, Chitosan and Related Enzymes, in Academic Press. 1984: Orlando. 6. Rinaudo, M., G. Pavlov, and J. Desbrieres, Influence of acetic acid concentration on the solubilization of chitosan. Polymer, 1999. 40(25): p. 7029-7032. 7. Paul W, S.C., Chitosan and alginate wound dressings:A short review. Trends Biomater Artif Organs, 2004. 18: p. 18-23. 8. Brown, M.A., M.R. Daya, and J.A. Worley, Experience with Chitosan Dressings in a Civilian EMS System. The Journal of Emergency Medicine, 2009. 37(1): p. 1-7. 9. Mattioli-Belmonte, M., et al., Chitin Nanofibrils Linked to Chitosan Glycolate as Spray, Gel, and Gauze Preparations for Wound Repair. Journal of Bioactive and Compatible Polymers, 2007. 22(5): p. 525-538. 10. Zhou, Y., et al., Electrospun Water-Soluble Carboxyethyl Chitosan/Poly(vinyl alcohol) Nanofibrous Membrane as Potential Wound Dressing for Skin Regeneration. Biomacromolecules, 2007. 9(1): p. 349-354. 11. Cai, Z.-x., et al., Fabrication of Chitosan/Silk Fibroin Composite Nanofibers for Wound-dressing Applications. International Journal of Molecular Sciences, 2010. 11(9): p. 3529-3539. 12. Kossovich, L.Y., Y. Salkovskiy, and I.V. Kirillova, Electrospun Chitosan Nanofiber Materials as Burn Dressing, in 6th World Congress of Biomechanics (WCB 2010).2010:Springer Berlin Heidelberg.p. 1212-1214. 13. Liu, B.-S., C.-H. Yao, and S.-S. Fang, Evaluation of a Non-Woven Fabric Coated with a Chitosan Bi-Layer Composite for Wound Dressing. Macromolecular Bioscience, 2008. 8(5): p. 432-440. 14. Wichterle O, L.D., Hydrophobic gels in biologic use. Nature, 1960.185:p. 117. 15. Berger, J., et al., Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications. European Journal of Pharmaceutics and Biopharmaceutics, 2004. 57(1): p. 19-34. 16. X. Chen, W.L., W. Zhong, Y. Lu, T. Yu, pH sensitivity and ion sensitivity of hydrogel based on complex-forming chitosan/silk fibroin interpenetrating. J. Appl. Polym. Sci, 1997. 65: p. 2257-2262. 17. Crescenzi, V., et al., New hydrogels based on carbohydrate and on carbohydrate-synthetic polymer networks. Polymer Gels and Networks, 1997. 5(3): p. 225-239. 18. M. N. Khalid, L.H., J. L. Agnely, J. L. Grossiord, G. Couarraze, Swelling properties and mechanical characterization of a semi-interpenetrating chitosan/polyethylene oxide network: Comparison with a chitosan reference gel. STP Pharm. Sci., 1999. 9: p. 359-364. 19. Wang, M., Y. Fang, and D. Hu, Preparation and properties of chitosan-poly(N-isopropylacrylamide) full-IPN hydrogels. Reactive and Functional Polymers, 2001. 48(1-3): p. 215-221. 20. Wang, M., et al., Preparation and properties of chitosan-poly(N-isopropylacrylamide) semi-IPN hydrogels. Journal of Polymer Science Part A: Polymer Chemistry, 2000. 38(3): p. 474-481. 21. Risbud, M., A. Hardikar, and R. Bhonde, Growth modulation of fibroblasts by chitosan-polyvinyl pyrrolidone hydrogel: Implications for wound management? Journal of Biosciences, 2000. 25(1): p. 25-30. 22. Murakami, K., et al., Hydrogel blends of chitin/chitosan, fucoidan and alginate as healing-impaired wound dressings. Biomaterials, 2010. 31(1): p. 83-90. 23. Boucard, N., et al., The use of physical hydrogels of chitosan for skin regeneration following third-degree burns. Biomaterials, 2007. 28(24): p. 3478-3488. 24. Ishihara, M., et al., Photocrosslinkable chitosan as a dressing for wound occlusion and accelerator in healing process. Biomaterials, 2002. 23(3): p. 833-840. 25. Mi, F.-L., et al., Fabrication and characterization of a sponge-like asymmetric chitosan membrane as a wound dressing. Biomaterials, 2001. 22(2): p. 165-173. 26. Mizuno K, Y.K., Yano K, Osada T, Saeki S, Takimoto N ,et al., Effect of chitosan film containing basic fibroblast growth factor on wound healing in genetically diabetic mice. J Biomed Mater Res, 2003. 64A: p. 177-181. 27. Ong, S.-Y., et al., Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties. Biomaterials, 2008. 29(32): p. 4323-4332. 28. Li, L.-H., et al., Preparation, characterization and antimicrobial activities of chitosan/Ag/ZnO blend films. Chemical Engineering Journal, 2010. 160(1): p. 378-382. 29. Sachlos E, C.J., Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. Eur Cells Mater, 2003. 5: p. 29-40. 30. Dagalakis, N., et al., Design of an artificial skin. Part III. Control of pore structure. Journal of Biomedical Materials Research, 1980. 14(4): p. 511-528. 31. Yannas, I.V., et al., Design of an artificial skin. II. Control of chemical composition. Journal of Biomedical Materials Research, 1980. 14(2): p. 107-132. 32. Yannas, I.V. and J.F. Burke, Design of an artificial skin. I. Basic design principles. Journal of Biomedical Materials Research, 1980. 14(1): p. 65-81. 33. Denkbaş, E.B., et al., Norfloxacin-loaded Chitosan Sponges as Wound Dressing Material. Journal of Biomaterials Applications, 2004. 18(4): p. 291-303. 34. Poison, A., A Theory for the Displacement of Proteins and Viruses with Polyethylene Glycol. Preparative Biochemistry, 1977. 7(2): p. 129 - 154. 35. WR Gombotz, W.G., TA Horbett, AS Hoffman, Protein adsorption to and elution from polyether surface. 1992. 36. Working Peter, K., et al., Safety of Poly(ethylene glycol) and Poly(ethylene glycol) Derivatives, in Poly(ethylene glycol). 1997, American Chemical Society. p. 45-57. 37. Abuchowski, A., et al., Alteration of immunological properties of bovine serum albumin by covalent attachment of polyethylene glycol. Journal of Biological Chemistry, 1977. 252(11): p. 3578-3581. 38. Lee, S.B., et al., Preparation and properties of polyelectrolyte complex sponges composed of hyaluronic acid and chitosan and their biological behaviors. Journal of Applied Polymer Science, 2003. 90(4): p. 925-932. 39. Mucha, M., Rheological properties of chitosan blends with poly(ethylene oxide) and poly(vinyl alcohol) in solution. Reactive and Functional Polymers, 1998. 38(1): p. 19-25. 40. Zhang, M., et al., Properties and biocompatibility of chitosan films modified by blending with PEG. Biomaterials, 2002. 23(13): p. 2641-2648. 41. Kolhe, P. and R.M. Kannan, Improvement in Ductility of Chitosan through Blending and Copolymerization with PEG: FTIR Investigation of Molecular Interactions. Biomacromolecules, 2002. 4(1): p. 173-180. 42. Mao, S., et al., Synthesis, characterization and cytotoxicity of poly(ethylene glycol)-graft-trimethyl chitosan block copolymers. Biomaterials, 2005. 26(32): p. 6343-6356. 43. Kulkarni, A.R., et al., A Novel Method for the Synthesis of the PEG-Crosslinked Chitosan with a pH-Independent Swelling Behavior. Macromolecular Bioscience, 2005. 5(10): p. 925-928. 44. Zhang, X., D. Yang, and J. Nie, Chitosan/polyethylene glycol diacrylate films as potential wound dressing material. International Journal of Biological Macromolecules, 2008. 43(5): p. 456-462. 45. Tanuma, H., et al., Preparation and characterization of PEG-cross-linked chitosan hydrogel films with controllable swelling and enzymatic degradation behavior. Carbohydrate Polymers, 2010. 80(1): p. 260-265. 46. Kiuchi, H., W. Kai, and Y. Inoue, Preparation and characterization of poly(ethylene glycol) crosslinked chitosan films. Journal of Applied Polymer Science, 2008. 107(6): p. 3823-3830. 47. Casettari, L., et al., Effect of PEGylation on the Toxicity and Permeability Enhancement of Chitosan. Biomacromolecules, 2010. 11(11): p. 2854-2865. 48. 趙壯飛, 劉江川, 劉鴻文, 呂美華, 醫用組織學彩色圖譜. 2001. 49. Michael H.Ross, L.J.R., Gordon I.Kaye, Histology: A Text and Atlas. 1998. 50. 范如霖, 范文峰, 曹光磊, 皮膚組織病理學. 1972. 51. Telles, D.d.S. A complete diagram of the human skin. Available from: http://en.wikipedia.org/wiki/Skin. 52. Kilbad. This is a hematoxylin and eosin stained slide at 10x of normal skin. Available from: http://en.wikipedia.org/wiki/File:Epidermis-delimited.JPG. 53. Mikael Haggstrom, b.o.w.b.W. Layers of the epidermis. Available from: http://en.wikipedia.org/wiki/File:Epidermal_layers.png. 54. This is a hematoxylin and eosin stained slide of normal epidermis. Available from: http://neuromedia.neurobio.ucla.edu. 55. Tsuboi, R. and D.B. Rifkin, Recombinant basic fibroblast growth factor stimulates wound healing in healing-impaired db/db mice. The Journal of Experimental Medicine, 1990. 172(1): p. 245-251. 56. Harari, J., Surgical Complications and Wound Healing in the Small Animal Practice, ed. J. Harari. Vol. 22. 1993: Blackwell Publishing Ltd. 57. The phases of cutaneous wound healing. Available from:http://www.pilonidal.org/aftercare/wound_healing_indepth.php. 58. Tsao, C.T., et al., Evaluation of chitosan/[gamma]-poly(glutamic acid) polyelectrolyte complex for wound dressing materials. Carbohydrate Polymers, 2011. 84(2): p. 812-819. 59. Adekogbe, I. and A. Ghanem, Fabrication and characterization of DTBP-crosslinked chitosan scaffolds for skin tissue engineering. Biomaterials, 2005. 26(35): p. 7241-7250. 60. Ciapetti, G., et al., Application of a combination of neutral red and amido black staining for rapid, reliable cytotoxicity testing of biomaterials. Biomaterials, 1996. 17(13): p. 1259-1264. 61. Roberts, M.J., M.D. Bentley, and J.M. Harris, Chemistry for peptide and protein PEGylation. Advanced Drug Delivery Reviews, 2002. 54(4): p. 459-476. 62. Kim, I.Y., et al., Evaluation of semi-interpenetrating polymer networks composed of chitosan and poloxamer for wound dressing application. International Journal of Pharmaceutics, 2007. 341(1-2): p. 35-43. 63. Nordtveit, R.J., K.M. Varum, and O. Smidsrod, Degradation of partially N-acetylated chitosans with hen egg white and human lysozyme. Carbohydrate Polymers, 1996. 29(2): p. 163-167. 64. Tomihata, K. and Y. Ikada, In vitro and in vivo degradation of films of chitin and its deacetylated derivatives. Biomaterials, 1997. 18(7): p. 567-575. 65. Pangburn, S.H., P.V. Trescony, and J. Heller, Lysozyme degradation of partially deacetylated chitin, its films and hydrogels. Biomaterials, 1982. 3(2): p. 105-108. 66. H. Rosen, J.K., K. Leong, R. Langer., ed. Controlled Release Systems: Fabrication Technology. 1988, CRC Press. 83. 67. Wang, J.W. and M.H. Hon, Biodegradation behavior and cytotoxicity of the composite membrane composed of β-dicalcium pyrophosphate and glucose mediated (polyethylene glycol/chitosan). Journal of Materials Science: Materials in Medicine, 2004. 15(2): p. 129-136. 68. Saneinejad, S. and M.S. Shoichet, Patterned glass surfaces direct cell adhesion and process outgrowth of primary neurons of the central nervous system. Journal of Biomedical Materials Research, 1998. 42(1): p. 13-19. 69. Zielinski, B., Chitosan as a matrix for mammalian cell encapsulation. Biomaterials, 1994. 15: p. 1049-56. 70. Davidson, R.L., K.A. O'Malley, and T.B. Wheeler, Polyethylene glycol-induced mammalian cell hybridization: Effect of polyethylene glycol molecular weight and concentration. Somatic Cell and Molecular Genetics, 1976. 2(3): p. 271-280. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30408 | - |
dc.description.abstract | 幾丁聚醣其具有良好的生物性質,例如生物相容性、生物降解性以及抗菌性,且幾丁聚醣對於血液有促進凝結止血的效果。然而幾丁聚醣的低機械性質限制其應用範圍。幾丁聚醣在酸性條件下會形成具有帶正電胺基(amine group)的聚電解質,可和帶負電的聚電解質或生長因子形成錯合物,或是和具有羧基(carboxylic acid)的物質藉由醯胺鍵(amide bond)形成交聯結構,以修飾(modify)幾丁聚醣材料的性質。
本研究選用不同分子量和比例的聚乙二醇交聯幾丁聚醣,探討其物理化學性質和生物性質。除此之外,在動物實驗方面以全層皮膚缺損作為模型發現聚乙二醇交聯幾丁聚醣薄膜有助於Male Balb/c小鼠傷口癒合。從病理組織切片觀察,幾丁聚醣敷料有刺激纖維母細胞的增生能力和抑制發炎細胞的作用,而聚乙二醇添加,對於小鼠背部上皮細胞轉移有增進的效果。 綜合以上,聚乙二醇交聯幾丁聚醣是極具潛力的創傷敷料。 | zh_TW |
dc.description.abstract | Chitosan has outstanding biological properties, including biocompatible, biodegradable and antibacterial ability. Moreover, chitosan has hemostatic ability for promoting blood to coagulate together. However, the applications of chitosan are limited for its low mechanical properties. Chitosan existing in acid environment becomes a cationic polyelectrolyte due to its amine group, it can easily form polyelectrolyte complexes with other anionic polyelectrolytes and growth factors, or form crosslink structure with carboxylic group by composing amide bond. Through this strategy, the properties of chitosan can be modified and enhanced.
In this research, we studied the properties of materials made by cross-linked chitosan with different poly(ethylene glycol)(PEG) molecular weight and various PEG to chitosan ratio. The physicochemical characterization and biological properties were studied for the PEG crosslinked chitosan films. Furthermore, an in vivo animal test using Male Balb/c mice showed rather good wound healing effect of PEG crosslinked chitosan film in the full-thickness skin defect model. From the histological examination, chitosan could suppress the infiltration of inflammatory cells and accelerate fibroblast proliferation while the PEG could enhance epithelial migration. Thus, PEG crosslinked chitosan films can be a potential wound dressing with excellent forming and enhanced wound healing. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T02:03:07Z (GMT). No. of bitstreams: 1 ntu-100-R98524082-1.pdf: 3257141 bytes, checksum: 8f29b2237c77afb3065f172e23c62cea (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 中文摘要 I
Abstract II 致謝 III 目次 IV 圖次 VII 表次 IX 第一章 簡介 1 第二章 文獻回顧 2 2-1 幾丁聚醣之簡介 2 2-2 幾丁聚醣於創傷敷料之應用 4 2-2-1 纖維型幾丁聚醣於創傷敷料之應用 6 2-2-2 水膠型幾丁聚醣於創傷敷料之應用 7 2-2-3 薄膜型幾丁聚醣於創傷敷料之應用 11 2-2-4 泡綿型幾丁聚醣於創傷敷料之應用 13 2-3 聚乙二醇之簡介 14 2-4皮膚構造之簡介[48-50] 17 2-5 傷口癒合機制之簡介[55] 19 第三章 實驗藥品與實驗儀器 22 3-1 實驗藥品 22 3-2 實驗儀器 24 第四章 實驗方法 25 4-1 聚乙二醇交聯幾丁聚醣製備方法 25 4-2 材料性質測試 28 4-2-1 傅立葉轉換紅外線光譜儀 28 4-2-2 熱重分析儀 28 4-2-3 微差掃描卡計儀 28 4-2-4 拉伸測試 28 4-2-5 澎潤比 28 4-2-6 水氣透過率 29 4-3 生物性質測試 30 4-3-1 生物降解性測試 30 4-3-2 細胞相容性測試 30 4-4 動物實驗 32 4-4-1 小傷口切傷動物實驗 32 4-4-2 大傷口切傷動物實驗 33 4-4-3 組織切片觀察 33 第五章 結果與討論 34 5-1 材料性質測試 34 5-1-1 傅立葉轉換紅外線光譜分析 34 5-1-2 熱性質分析 38 5-1-2-1 熱重分析 38 5-1-2-2 微差掃描卡計分析 40 5-1-3 拉伸測試分析 42 5-1-4 澎潤比分析 44 5-1-5 水氣透過率分析 46 5-2生物性質測試 48 5-2-1生物降解性測試分析 48 5-2-2細胞相容性測試分析 50 5-3動物實驗 51 5-3-1小傷口切傷動物實驗分析 51 5-3-1-1 小傷口切傷動物實驗之表面癒合肉眼觀察 51 5-3-1-2 正常皮膚之病理組織切片觀察 53 5-3-1-3 小傷口切傷動物實驗病理組織切片觀察 54 5-3-2大傷口切傷動物實驗分析 62 5-3-2-1 大傷口切傷之表面癒合肉眼觀察 62 5-3-2-2 大傷口切傷傷口之病理組織切片觀察 64 第六章 結論 71 參考文獻 72 | |
dc.language.iso | zh-TW | |
dc.title | 聚乙二醇交聯幾丁聚醣作為創傷敷料之應用研究 | zh_TW |
dc.title | Crosslinking chitosan with PEG for wound dressing development study | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳銘芳(Ming-Fang Wu),張志豪(Chih-Hao Chang) | |
dc.subject.keyword | 幾丁聚醣,聚乙二醇,交聯,水膠,創傷敷料, | zh_TW |
dc.subject.keyword | chitosan,PEG,cross-link,hydrogel,wound dressing, | en |
dc.relation.page | 76 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-02 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 3.18 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。