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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔣丙煌 | |
| dc.contributor.author | Hai-Ju Wu | en |
| dc.contributor.author | 吳蕙如 | zh_TW |
| dc.date.accessioned | 2021-06-13T00:37:13Z | - |
| dc.date.available | 2009-07-31 | |
| dc.date.copyright | 2007-07-26 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-24 | |
| dc.identifier.citation | 李汪章. 2000. 以事業廢棄物資源化建立企業競爭優勢之研究-以台灣水產業為例. 台北: 元智大學管理研究所碩士論文.
周俊吉. 2001. 膠原蛋白為基材之創傷敷料研究. 國立陽明大學醫學工程研究所碩士論文. 周燕輝. 1987. 皮膚病. 正中書局，台北. 施英隆 & 范宜琮. 2001. 納豆-神奇之保健食品. 生物資源生物技術 4(1):37-45. 范繼宗. 2005. 納豆對活性氧之抗致突變性及抗氧化性. 台北: 台灣大學食品科技研究所碩士班論文. 楊雨滋 & 焦永若. 1995. 單角革單棘魨魚皮資源的開發與利用. 中國皮革 24:16. 蘇娟娟. 2003. 米酒糟蛋白質對人類真皮層纖維母細胞生長的影響. 花蓮: 東華大學生物技術研究所碩士論文. Abamba G. 1993. Poucher's perfumes, Cosmetics and Soaps. Afaq F, Adhami VM, Ahmad N & Mukhtar H. 2003. Inhibition of ultraviolet B-mediated activation of nuclear factor kappaB in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-gallate. Oncogene 22(7):1035-1044. Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh N, Shibuya M & Fukami Y. 1987. Genistein, a specific inhibitor of tyrosine-specific protein kinases. J biol chem 262(12):5592-5595. Alessio HM & Goldfarb AH. 1988. Lipid peroxidation and scavenger enzymes during exercise: adaptive response to training. J Appl Physiol 64(4):1333-1336. Ando H, Kondoh H, Ichihashi M & Hearing VJ. 2007. Approaches to identify inhibitors of melanin biosynthesis via the quality control of tyrosinase. J invest dermatol 127(4):751-761. Arjmandi BH & Smith BJ. 2002. Soy isoflavones' osteoprotective role in postmenopausal women: mechanism of action. J Nutr Biochem 13(3):130-137. Bauza E, Oberto G, Berghi A, Dal CF & Domloge N. 2004. Collagen-like peptide exhibits a remarkable antiwrinkle effect on the skin when topically applied: in vivo study. Int j tissue react 26(3-4):105-111. Beehler BC, Przybyszewski J, Box HB & Kulesz-Martin MF. 1992. Formation of 8-hydroxydeoxyguanosine within DNA of mouse keratinocytes exposed in culture to UVB and H2O2. Carcinogenesis 13(11):2003-2007. Biesalski HK. 2002. Free radical theory of aging. Curr opin clin nutr metabol care 5(1):5-10. Birt DF, Hendrich S & Wang W. 2001. Dietary agents in cancer prevention: flavonoids and isoflavonoids. Pharmacol ther 90(2-3):157-177. Boersma BJ, Barnes S, Kirk M, Wang CC, Smith M, Kim H, Xu J, Patel R & Darley-Usmar VM. 2001. Soy isoflavonoids and cancer -- metabolism at the target site. Mutat res 480-481:121-127. Bowden GT. 2004. Prevention of non-melanoma skin cancer by targeting ultraviolet-B-light signalling. Nature reviews 4(1):23-35. Cai Q & Wei H. 1996. Effect of dietary genistein on antioxidant enzyme activities in SENCAR mice. Nutr cancer 25(1):1-7. Chen Q, Chen QX, Qiu L, Song KK & Huang H. 2003. Inhibitory effect of 4-cyanobenzaldehyde and 4-cyanobenzoic acid on mushroom (Agaricus bisporus) tyrosinase. J protein chem 22(7-8):607-612. Chung JH, Seo JY, Choi HR, Lee MK, Youn CS, Rhie G, Cho KH, Kim KH, Park KC & Eun HC. 2001. Modulation of skin collagen metabolism in aged and photoaged human skin in vivo. J invest dermatol 117(5):1218-1224. Church F, Swaisgood H, Porter D & Catignani G. 1983 Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. J Dairy Sci 66:1219-1227. Dash S, Nath LK, Bhise S & Bhuyan N. 2005. Antioidant and antimicrobial activities of Heracleum nepalense D Don root. Trop J Pharm Res 4(1):341-347. Davies KJ, Quintanilha AT, Brooks GA & Packer L. 1982. Free radicals and tissue damage produced by exercise. Biochem biophys res commun 107(4):1198-1205. Del Maestro R, Thaw HH, Bjork J, Planker M & Arfors KE. 1980. Free radicals as mediators of tissue injury. Acta physiol. Scand 492:43-57. Doerge DR & Chang HC. 2002. Inactivation of thyroid peroxidase by soy isoflavones, in vitro and in vivo. J chromatogr 777(1-2):269-279. El-Chemaly S, Salathe M, Baier S, Conner GE & Forteza R. 2003. Hydrogen peroxide-scavenging properties of normal human airway secretions. Am j respir crit care med 167(3):425-430. Ellouz Y, Bayoudh A, Kammoun S, Gharsallah N & Nasri M. 2001. Production of protease by Bacillus subtilis grown on sardinelle heads and viscera flour. Bioresource technology 80(1):49-51. Eriksson CE & Na A. 1995. Antioxidant agents in raw materials and processed foods. Biochemical Society symposium 61:221-234. Gasowska B, Wojtasek H, Hurek J, Drag M, Nowak K & Kafarski P. 2002. Redox reaction between amino-(3,4-dihydroxyphenyl)methyl phosphonic acid and dopaquinone is responsible for the apparent inhibitory effect on tyrosinase. Eur j biochem 269(16):4098-4104. Gilchrest BA. 1989. Skin aging and photoaging: an overview. J Am Acad Dermatol 21(3 Pt 2):610-613. Guo J, Sun Y & Su Y. 2002. [Preparation of natto and its function in health care]. Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 25(1):61-64. Halliwell B. 1999. Antioxidant defence mechanisms: from the beginning to the end (of the beginning). Free radical research 31(4):261-272. Harman D. 1992. Free radical theory of aging. Mutation research 275(3-6):257-266. Harman D. 2003. The free radical theory of aging. Antioxid redox signal 5(5):557-561. Hou WC, Lee MH, Chen HJ, Liang WL, Han CH, Liu YW & Lin YH. 2001. Antioxidant activities of dioscorin, the storage protein of yam (Dioscorea batatas Decne) tuber. J agric food chem 49(10):4956-4960. Inoue M. 2002. Free radical theory of aging. Nippon Ronen Igakkai zasshi 39(1):36-38. Jenkins RR. 1988. Free radical chemistry. Relationship to exercise. Sports medicine (Auckland, N.Z 5(3):156-170. Kamoun A, Landeau JM, Godeau G, Wallach J, Duchesnay A, Pellat B & Hornebeck W. 1995. Growth stimulation of human skin fibroblasts by elastin-derived peptides. Cell adhesion and communication 3(4):273-281. Kamsler A & Segal M. 2004. Hydrogen peroxide as a diffusible signal molecule in synaptic plasticity. Mol neurobiol 29(2):167-178. Katayama K, Armendariz-Borunda J, Raghow R, Kang AH & Seyer JM. 1993. A pentapeptide from type I procollagen promotes extracellular matrix production. J biol chem 268(14):9941-9944. Katiyar SK, Afaq F, Azizuddin K & Mukhtar H. 2001. Inhibition of UVB-induced oxidative stress-mediated phosphorylation of mitogen-activated protein kinase signaling pathways in cultured human epidermal keratinocytes by green tea polyphenol (-)-epigallocatechin-3-gallate. Toxicol appl pharmacol 176(2):110-117. Katiyar SK, Perez A & Mukhtar H. 2000. Green tea polyphenol treatment to human skin prevents formation of ultraviolet light B-induced pyrimidine dimers in DNA. Clin Cancer Res 6(10):3864-3869. Kawanishi S, Inoue S & Yamamoto K. 1989. Hydroxyl radical and singlet oxygen production and DNA damage induced by carcinogenic metal compounds and hydrogen peroxide. Biol trace elem. res 21:367-372. Khaziev AF, Mikhailova NA & Alsynbaev MM. 2002. Study of the effectiveness of methods of purification of metalloprotease produced by Bacillus subtilis. Zhurnal mikrobiologii, epidemiologii, i immunobiologii (6):75-77. Khaziev AF, Mikhailova NA & Alsynbaev MM. 2003. [Characterization of highly purified metalloprotease produced by Bacillus subtilis]. Zhurnal mikrobiologii, epidemiologii, i immunobiologii (3):91-93. Kiers JL, Van Laeken AE, Rombouts FM & Nout MJ. 2000. In vitro digestibility of bacillus fermented soya bean. Int j food microbiol 60(2-3):163-169. Kim SK, Kim YT, Byun HG, Nam KS, Joo DS & Shahidi F. 2001. Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin. J agric food chem 49(4):1984-1989. Kligman LH. 1986. Photoaging. Manifestations, prevention, and treatment. Dermatologic clinics 4(3):517-528. Kong B & Xiong YL. 2006. Antioxidant activity of zein hydrolysates in a liposome system and the possible mode of action. J agric food chem 54(16):6059-6068. Kubo I & Kinst-Hori I. 1999. Tyrosinase inhibitory activity of the olive oil flavor compounds. J agric food chem 47(11):4574-4578. Kubo I, Kinst-Hori I, Chaudhuri SK, Kubo Y, Sanchez Y & Ogura T. 2000. Flavonols from Heterotheca inuloides: tyrosinase inhibitory activity and structural criteria. Biomed chem 8(7):1749-1755. Leyden JJ. 2002. skin care benefits of copper peptide containing facial cream. Presented at the American Academy of Dermatology 60th Annual Meeting; New Orleans, LA. Li J, Zheng RL, Liu ZM & Jia ZJ. 1992. Scavenging effects of phenylpropanoid glycosides on superoxide and its antioxidation effect. Zhongguo yao li xue bao = Acta pharmacologica Sinica 13(5):427-430. Linsenmayer TF, Gibney E, Gordon MK, Marchant JK, Hayashi M & Fitch JM. 1990. Extracellular matrices of the developing chick retina and cornea. Localization of mRNAs for collagen types II and IX by in situ hybridization. Invest ophthalmol visual sci 31(7):1271-1276. Lintner K. 2002. Promoting production in the extracellular matrix without compromising barrier. Cutis 70(6 Suppl):13-16; discussion 21-13. Lubec G. 1996. The hydroxyl radical: from chemistry to human disease. J Investig Med 44(6):324-346. Lupo MP. 2005. Cosmeceutical peptides. Dermatol Surg 31(7 Pt 2):832-836; discussion 836. Macedo AJ, da Silva WO, Gava R, Driemeier D, Henriques JA & Termignoni C. 2005. Novel keratinase from Bacillus subtilis S14 exhibiting remarkable dehairing capabilities. Appl environ microbiol 71(1):594-596. Markovits J, Linassier C, Fosse P, Couprie J, Pierre J, Jacquemin-Sablon A, Saucier JM, Le Pecq JB & Larsen AK. 1989. Inhibitory effects of the tyrosine kinase inhibitor genistein on mammalian DNA topoisomerase II. Cancer research 49(18):5111-5117. Martone CB, Perez Borla O & Sanchez JJ. 2005. Fishery by-product as a nutrient source for bacteria and archaea growth media. Bioresource technology 96(3):383-387. Mendis E, Rajapakse N, Byun HG & Kim SK. 2005a. Investigation of jumbo squid (Dosidicus gigas) skin gelatin peptides for their in vitro antioxidant effects. Life sciences 77(17):2166-2178. Mendis E, Rajapakse N & Kim SK. 2005b. Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate. J agric food chem 53(3):581-587. Miyachi Y. 1995. Photoaging from an oxidative standpoint. Journal of dermatological science 9(2):79-86. Mullan PB, Gardiner JC, Rosenman K, Zhu Z & Swanson GM. 1996. Skin cancer prevention and detection practices in a Michigan farm population following an educational intervention. J Rural Health 12(4 Suppl):311-320. Myllyharju J & Kivirikko KI. 2004. Collagens, modifying enzymes and their mutations in humans, flies and worms. Trends Genet 20(1):33-43. Njieha FK, Morikawa T, Tuderman L & Prockop DJ. 1982. Partial purification of a procollagen C-proteinase. Inhibition by synthetic peptides and sequential cleavage of type I procollagen. Biochemistry 21(4):757-764. Perrin A, Bauza E, Dal Farra C & Domloge N. 2004. Stimulating effect of collagen-like peptide on the extracellular matrix of human skin: histological studies. Int j tissue react 26(3-4):97-104. Persikov AV, Ramshaw JA & Brodsky B. 2000. Collagen model peptides: Sequence dependence of triple-helix stability. Biopolymers 55(6):436-450. Pervaiz S & Clement MV. 2007. Superoxide anion: Oncogenic reactive oxygen species? Int J Biochem Cell Biol. Pickart L & Thaler MM. 1980. Growth-modulating tripeptide (glycylhistidyllysine): association with copper and iron in plasma, and stimulation of adhesiveness and growth of hepatoma cells in culture by tripeptide-metal ion complexes. J cell physiol 102(2):129-139. Qian ZJ, Jung WK & Kim SK. 2007. Free radical scavenging activity of a novel antioxidative peptide purified from hydrolysate of bullfrog skin, Rana catesbeiana Shaw. Bioresource technology. Rabe JH, Mamelak AJ, McElgunn PJ, Morison WL & Sauder DN. 2006. Photoaging: mechanisms and repair. J Am Acad Dermatol 55(1):1-19. Ramshaw JA, Werkmeister JA & Glattauer V. 1996. Collagen-based biomaterials. Biotechnol genet eng rev 13:335-382. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M & Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radic biol med 26(9-10):1231-1237. Rittie L & Fisher GJ. 2002. UV-light-induced signal cascades and skin aging. Ageing research reviews 1(4):705-720. Rorsman. H. 1979. Dermatology. Chicago, Ill. : Distributed by Year Book Medical. Sakanaka S, Tachibana Y, Ishihara N & Juneja LR. 2005. Antioxidant properties of casein calcium peptides and their effects on lipid oxidation in beef homogenates. J agric food chem 53(2):464-468. Schallmey M, Singh A & Ward OP. 2004. Developments in the use of Bacillus species for industrial production. Can j microbiol 50(1):1-17. Scharffetter-Kochanek K, Wlaschek M, Brenneisen P, Schauen M, Blaudschun R & Wenk J. 1997. UV-induced reactive oxygen species in photocarcinogenesis and photoaging. Biological chemistry 378(11):1247-1257. Schurink M, van Berkel WJ, Wichers HJ & Boeriu CG. 2007. Novel peptides with tyrosinase inhibitory activity. Peptides 28(3):485-495. Shaginian KA, Izotova LS, Iomantac Iu V, Strongin A & Stepanov VM. 1980. Metalloproteinase from Bacillus subtilis: - 'intracellular' and extracellular enzymes. Biokhimiia (Moscow, Russia) 45(11):2083-2095. Simeon A, Emonard H, Hornebeck W & Maquart FX. 2000. The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life sciences 67(18):2257-2265. Sire JY & Akimenko MA. 2004. Scale development in fish: a review, with description of sonic hedgehog (shh) expression in the zebrafish (Danio rerio). Int j dev biol 48(2-3):233-247. Sorrell JM & Caplan AI. 2004. Fibroblast heterogeneity: more than skin deep. J Cell Sci 117:667-675. Stadtman ER & Levine RL. 2003. Free radical-mediated oxidation of free amino acids and amino acid residues in proteins. Amino acids 25(3-4):207-218. Svobodova A, Walterova D & Vostalova J. 2006. Ultraviolet light induced alteration to the skin. Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia 150(1):25-38. Sweat F, Puchtler H & Rosenthal SI. 1964. Sirius Red F3ba as a Stain for Connective Tissue. Arch. pathol 78:69-72. Tajima S, Wachi H, Uemura Y & Okamto K. 1997. Modulation by elastin peptide VGVAPG of cell proliferation and elastin expression in human skin fibroblasts. Arch Dermatol Res 289:489-492. Tran LH & Nagano H. 2002. Isolation and characteristics of Bacillus subtilis CN2 and its collagenase production. J food sci 67(3):1184-1187. van der Rest M, Dublet B & Champliaud MF. 1990. Fibril-associated collagens. Biomaterials 11:28-31. Varani J, Perone P, Fligiel SE, Fisher GJ & Voorhees JJ. 2002. Inhibition of type I procollagen production in photodamage: correlation between presence of high molecular weight collagen fragments and reduced procollagen synthesis. J invest dermatol 119(1):122-129. Varela H, Ferrari MD, Belobrajdic L, Vazquez A & Loperena ML. 1997. Skin unhairing proteases of Bacillus subtilis: production and partial characterization. Biotechnology Letters 19(8):755-758. Vayalil PK, Mittal A, Hara Y, Elmets CA & Katiyar SK. 2004. Green tea polyphenols prevent ultraviolet light-induced oxidative damage and matrix metalloproteinases expression in mouse skin. J invest dermatol 122(6):1480-1487. Wickens AP. 2001. Ageing and the free radical theory. Respir physiol 128(3):379-391. Winterbourn CC. 1995. Toxicity of iron and hydrogen peroxide: the Fenton reaction. Toxicol lett 82-83:969-974. Yamaguchi T, Takamura H, Matoba T & Terao J. 1998. HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl. Bioscience, biotechnology, and biochemistry 62(6):1201-1204. Yin L-J. 2005. Effect of rice koji fermentation on the characteristics of mackerel muscle. J Fish Soc 34(4):341-354. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29053 | - |
| dc.description.abstract | 本研究乃探討帶鱗單角革單棘魨魚皮以納豆菌發酵產物的抗氧化能力與其對皮膚纖維母細胞的影響。結果顯示，納豆菌能有效利用帶鱗單角革單棘魨魚皮為培養基質，並產生蛋白質水解物。有關DPPH、ABT+、超氧陰離子、過氧化氫與氫氧自由基的清除能力，均隨著發酵時間而提昇，而發酵時間延長對於清除過氧化氫的能力最有助益。對於還原力與酪氨酸酶活性的抑制效果則以發酵72小時較佳，且發酵72小時後的樣品能促進皮膚纖維母細胞超氧岐化酶酵素的活性，其能力可能來自於魚皮蛋白質水解物。在促進皮膚纖維母細胞增生方面，以發酵24小時最佳，在500μg/mL濃度下可提升約30%的細胞數。各發酵樣品皆能促進皮膚纖維母細胞原膠原蛋白合成，而發酵72小時促進效果最佳。然而，若先以UVA照射皮膚纖維母細胞，使其在受損的狀況下，未發酵之產物對細胞之修復能力有最佳之功效。但是，發酵24與48小時樣品亦兼具有促進受損之皮膚纖維母細胞存活與原膠原蛋白生成之功能。因此，利用納豆菌發酵帶鱗單角革單棘魨魚皮後，確實可得到具有皮膚保健功能的產物，並且在不同的發酵時間下得到的產物，可能可以分別用在皮膚美白與抗老化的保養品中。 | zh_TW |
| dc.description.abstract | The objective of this study was to produce functional products for skin care by fermenting the skin of Aluterus monoceros by Bacillus subtilis natto. The fermentation products were evaluated for their antioxidant properties and effect on procollagen synthesis of skin fibroblast.
The results showed a dramatic increase in scavenging abilities of DPPH, ABT+, superoxide anion, hydrogen peroxide and hydroxyl free radical of the fermentation products. Increase of fermentation time had most significant effect on hydrogen peroxide scavenging ability as compared to other free radicals. As for the reducing power and tyrosinase inhibition activity, the 72h fermentation product was most effective. The 72h product also improved the superoxide dismutase activity of the skin fibroblast. A 30% increase in skin fibroblast proliferation was observed on treatment with 24 hrs fermentation product at a concentration of 500μg/ml. While, fermentation product from 72 hr exhibited maximum procollagen synthesis by skin fibroblast. On irradiation with UVA, skin fibroblasts treated with un-fermented fish skin extract had the most profound effect on the repairing of the UVA-damaged cells. However, the 24 h- and 48 h- fermented products also showed an improved cell viability and procollagen synthesis then untreated cells. Hence, fermentation of the skin of Aluterus monoceros by B. subtilis natto could produce functional products of skin care, and the products with different fermentation times can be used in skin whitening and anti-ageing cosmetics. | en |
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| dc.description.tableofcontents | 中文摘要------------------------------------------------------------------------------ i
英文摘要------------------------------------------------------------------------------ ii 一、 緒論------------------------------------------------------------------------------ 1 二、 文獻回顧------------------------------------------------------------------------ 2 1. 帶鱗魚皮簡介-------------------------------------------------------------------- 2 2. 納豆菌簡介------------------------------------------------------------------------ 4 3. 皮膚的構造與功能--------------------------------------------------------------- 5 3.1 表皮層----------------------------------------------------------------------------- 5 3.1.1 黑色素與膚色------------------------------------------------------------------ 5 3.2 真皮層----------------------------------------------------------------------------- 7 3.2.1 皮膚纖維母細胞--------------------------------------------------------------- 8 3.2.2 膠原蛋白------------------------------------------------------------------------ 8 3.2.2.1 膠原蛋白結構與組成------------------------------------------------------- 8 3.2.2.2 膠原蛋白生合成------------------------------------------------------------- 9 3.3 皮下組織-------------------------------------------------------------------------- 10 4. 皮膚的老化------------------------------------------------------------------------- 10 4.1 老化學說-------------------------------------------------------------------------- 10 4.2 自由基的種類-------------------------------------------------------------------- 11 4.3 人體抗氧化防禦系統與抗氧化酵素----------------------------------------- 13 4.4 抗氧化物-------------------------------------------------------------------------- 15 4.4.1 蛋白質、胜肽與胺基酸抗氧化能力--------------------------------------- 15 5. 皮膚老化---------------------------------------------------------------------------- 16 5.1 紫外線與皮膚老化-------------------------------------------------------------- 17 5.2 抗氧化物質與皮膚老化-------------------------------------------------------- 18 6. 胜肽鏈與膠原蛋白增生---------------------------------------------------------- 19 三、 材料與方法--------------------------------------------------------------------- 21 實驗架構-------------------------------------------------------------------------------- 21 第一部份：以納豆菌發酵單角革單棘魨魚皮產生發酵產物 （一） 實驗材料---------------------------------------------------------------------- 24 （二） 藥品---------------------------------------------------------------------------- 24 （三） 實驗器材與儀器------------------------------------------------------------- 25 （四） 實驗方法---------------------------------------------------------------------- 26 （i） 單角革單棘魨魚皮基本成份分析------------------------------------- 26 1. 水分含量---------------------------------------------------------------------------- 26 2. 粗灰分------------------------------------------------------------------------------- 26 3. 粗脂肪------------------------------------------------------------------------------ 26 4. 粗蛋白------------------------------------------------------------------------------ 26 （ii） 以納豆菌發酵單角革單棘魨魚皮 1. 菌種活化及保存------------------------------------------------------------------ 27 2. 魚皮發酵物製備------------------------------------------------------------------ 27 3. 菌數測定--------------------------------------------------------------------------- 28 4. 胜肽濃度測定--------------------------------------------------------------------- 28 5. 蛋白質濃度測定------------------------------------------------------------------ 28 6. 明膠含量測定--------------------------------------------------------------------- 29 第二部份： 發酵物抗氧化能力與對皮膚纖維母細胞的影響 （i） 抗氧化能力分析 （一） 藥品----------------------------------------------------------------------------- 29 （二） 實驗方法----------------------------------------------------------------------- 30 1. 清除DPPH自由基能力測定----------------------------------------------------- 30 2. 清除ABT＋自由基能力測定----------------------------------------------------- 31 3. 清除超氧陰離子能力測定------------------------------------------------------- 31 4. 清除過氧化氫能力測定---------------------------------------------------------- 32 5. 清除氫氧自由基能力測定------------------------------------------------------- 32 6. 還原力測定------------------------------------------------------------------------- 33 7. 抑制酪氨酸酶能力測定---------------------------------------------------------- 33 （ii） 對皮膚纖維母細胞的影響 （一） 實驗細胞株-------------------------------------------------------------------- 34 （二） 藥品----------------------------------------------------------------------------- 34 （三） 實驗方法----------------------------------------------------------------------- 36 1. 細胞實驗樣品製備---------------------------------------------------------------- 36 2. CCD-966SK細胞之冷凍、解凍與培養--------------------------------------- 36 3. 發酵凍乾物對CCD-966SK細胞之毒性與增生------------------------------ 37 4. 在無UVA照射下CCD-966SK細胞之原膠原蛋白合成--------------------- 37 5. UVA照射下CCD-966SK細胞存活率------------------------------------------ 38 5.1 UVA照射裝置架設---------------------------------------------------------------- 38 5.2 實驗步驟---------------------------------------------------------------------------- 38 6. 原膠原蛋白含量測定------------------------------------------------------------- 39 7. 超氧歧化酶活性測定------------------------------------------------------------- 39 8. 過氧化氫酶活性測定------------------------------------------------------------- 40 統計分析--------------------------------------------------------------------------------- 41 四、結果與討論------------------------------------------------------------------------ 42 第一部份：以納豆菌發酵單角革單棘魨魚皮產生發酵產物 1. 帶鱗魚皮基本成份---------------------------------------------------------------- 42 2. 發酵過程及其變化--------------------------------------------------------------- 42 （1） 納豆菌數----------------------------------------------------------------------- 42 （2） pH------------------------------------------------------------------------------- 44 （3） 凍乾產物回收率、外觀與氣味-------------------------------------------- 46 3. 總蛋白質、胜肽與明膠含量---------------------------------------------------- 47 第二部份： 發酵物抗氧化與抑制酪氨酸酶的效果 （A） 抗氧化能力--------------------------------------------------------------------- 51 a. 清除DPPH自由基能力------------------------------------------------------ 51 b. 清除ABT＋自由基能力------------------------------------------------------ 53 c. 清除超氧陰離子能力------------------------------------------------------- 56 d. 清除過氧化氫能力---------------------------------------------------------- 56 e. 清除氫氧自由基能力------------------------------------------------------- 59 f. 還原力------------------------------------------------------------------------- 60 （B） 抑制酪氨酸酶能力------------------------------------------------------------ 64 第三部份： 發酵物對皮膚纖維母細胞之影響 （A） 對皮膚纖維母細胞之毒性與增生------------------------------------------ 67 （B） 對皮膚纖維母細胞超氧歧化沒與過氧化氫酶活性之影響------------ 73 （C） 無UVA照射下對皮膚纖維母細胞原膠原蛋白合成之影響------------ 76 （D） 對UVA照射後皮膚纖維母細胞之存活力影響--------------------------- 80 （E） 對UVA照射後皮膚纖維母細胞原膠原蛋白合成之影響--------------- 80 五、 結論--------------------------------------------------------------------------------- 84 參考文獻----------------------------------------------------------------------------------- 85 附錄----------------------------------------------------------------------------------------- 92 | |
| dc.language.iso | zh-TW | |
| dc.subject | 抗氧化 | zh_TW |
| dc.subject | 皮膚纖維母細胞 | zh_TW |
| dc.subject | 原膠原蛋白 | zh_TW |
| dc.subject | 納豆菌 | zh_TW |
| dc.subject | 單角魨單棘魨 | zh_TW |
| dc.subject | Aluterus monoceros | en |
| dc.subject | antioxidant | en |
| dc.subject | skin fibroblast | en |
| dc.subject | procollagen | en |
| dc.subject | Bacillus subtilis natto | en |
| dc.title | 以納豆菌發酵帶鱗單角革單棘魨魚皮生產具有皮膚保健功能之產物 | zh_TW |
| dc.title | Fermentation of the skin of Aluterus monoceros by Bacillus subtilis natto to manufacture functional products for human skin | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳榮輝,孫璐西,葉安義 | |
| dc.subject.keyword | 皮膚纖維母細胞,原膠原蛋白,納豆菌,單角魨單棘魨,抗氧化, | zh_TW |
| dc.subject.keyword | skin fibroblast,procollagen,Bacillus subtilis natto,Aluterus monoceros,antioxidant, | en |
| dc.relation.page | 91 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-26 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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|---|---|---|---|
| ntu-96-1.pdf 未授權公開取用 | 1.12 MB | Adobe PDF |
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