鑑別台灣主要稉稻品種的InDel標記之開發

Abstract

為鑑別台灣地區主要良質米稉稻品種，本試驗一共篩選了10 組SNP (Single Nucleotide Polymorphisms) 標記，152組InDel (insertion / deletion) 標記，156 組STS (Sequence tagged sites) 標記。最後在21個稉稻品種中得到具多型性的4組SNP標記、30組InDel標記和25 組STS標記，共59組可在一般電泳膠片中高通量分析之多型性標記；排除顯隱性多型性、需較精細調控的四引子系統及多型性重複的標記後，共有34組共顯性多型性標記。利用此34組獨特多型性可選出五組各含7組標記的最小鑑別組合，可完全鑑別21個稉稻品種。根據所得的共顯性標記應用於混合樣品分析，對於混雜率的檢測能力可以達到10%，對於品種檢查時樣品數較大的分析需求也能具有快速分析的優點。

In order to identify the major japonica rice cultivars in Taiwan, 10 single nucleotide polymorphisms (SNP) markers, 152 insertion/deletion (InDel) markers and 156 sequence tagged sites (STS) markers were screened against 21 japonica rice cultivars. Among the 318 markers screened, 59 of them are polymorphic, including 4 SNP markers, 30 InDel markers, and 25 STS markers. After eliminating dominant markers, tetra primer markers and replications of polymorphisms, there are 34 co-dominant markers showing unique polymorphic patterns. Five minimum sets of markers that can identify each of the 21 japonica rice cultivars completely from these 34 unique polymorphic markers contains only 7 markers. The sensitivity for testing bulk samples reach 10% while using these co-dominant markers, and therefore suitable for analyzing large amount of samples during variety testing.