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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27886
標題: | 鑑別台灣主要稉稻品種的InDel標記之開發 Development of InDel Markers for Variety Identification amomg Major Japonica Rice Cultivars in Taiwan |
作者: | Tsai Yi Yen 顏采衣 |
指導教授: | 胡凱康 |
關鍵字: | 稉,稻,分子標記,品種鑑別, japonica rice,marker,variety Identification, |
出版年 : | 2007 |
學位: | 碩士 |
摘要: | 為鑑別台灣地區主要良質米稉稻品種,本試驗一共篩選了10 組SNP (Single Nucleotide Polymorphisms) 標記,152組InDel (insertion / deletion) 標記,156 組STS (Sequence tagged sites) 標記。最後在21個稉稻品種中得到具多型性的4組SNP標記、30組InDel標記和25 組STS標記,共59組可在一般電泳膠片中高通量分析之多型性標記;排除顯隱性多型性、需較精細調控的四引子系統及多型性重複的標記後,共有34組共顯性多型性標記。利用此34組獨特多型性可選出五組各含7組標記的最小鑑別組合,可完全鑑別21個稉稻品種。根據所得的共顯性標記應用於混合樣品分析,對於混雜率的檢測能力可以達到10%,對於品種檢查時樣品數較大的分析需求也能具有快速分析的優點。 In order to identify the major japonica rice cultivars in Taiwan, 10 single nucleotide polymorphisms (SNP) markers, 152 insertion/deletion (InDel) markers and 156 sequence tagged sites (STS) markers were screened against 21 japonica rice cultivars. Among the 318 markers screened, 59 of them are polymorphic, including 4 SNP markers, 30 InDel markers, and 25 STS markers. After eliminating dominant markers, tetra primer markers and replications of polymorphisms, there are 34 co-dominant markers showing unique polymorphic patterns. Five minimum sets of markers that can identify each of the 21 japonica rice cultivars completely from these 34 unique polymorphic markers contains only 7 markers. The sensitivity for testing bulk samples reach 10% while using these co-dominant markers, and therefore suitable for analyzing large amount of samples during variety testing. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27886 |
全文授權: | 有償授權 |
顯示於系所單位: | 農藝學系 |
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