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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 胡凱康 | |
dc.contributor.author | Tsai Yi Yen | en |
dc.contributor.author | 顏采衣 | zh_TW |
dc.date.accessioned | 2021-06-12T18:26:00Z | - |
dc.date.available | 2009-08-28 | |
dc.date.copyright | 2007-08-28 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-08-10 | |
dc.identifier.citation | 吳岱融,2004。以SSR分子標記測量水稻品種內之遺傳均一性,國立台灣大學農藝所碩士論文。
吳東鴻,2006。水稻於良種繁殖制度下之遺傳變異,國立台灣大學農藝所碩士論文。 陳恆,2002。水稻台粳品種間之親緣關係,國立台灣大學農藝所碩士論文。 農業統計年報,2006農業統計年報,行政院農業委員會。 Ayliffe, M.A., G.J. Lawrence, J.G. Ellis, and A.J. Pryor. 1994. Heteroduplex Molecules Formed between Allelic Sequences Cause Nonparental Rapd Bands. Nucleic Acids Research 22: 1632-1636. Doyle, J.J., J. L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15. Feltus, F.A., J. Wan, S.R. Schulze, J.C. Estill, N. Jiang, and A.H. Paterson. 2004. An SNP resource for rice genetics and breeding based on subspecies Indica and Japonica genome alignments. Genome Research 14: 1812-1819. Graham, R., M. Liew, C. Meadows, E. Lyon, and C.T. Wittwer. 2005. Distinguishing different DNA heterozygotes by high-resolution melting. Clin Chem 51: 1295-8. International Rice Genome Sequencing Project . 2007 http://rgp.dna.affrc.go.jp/IRGSP/) International Rice Genome Sequencing Project . 2005. The map-based sequence of the rice genome. Nature 436: 793-800. Jung, J.K. 2005. Development of Single Nucleotide Polymorphism Markers for Discrimination of Rice (Oryza sativa L.) Cultivar., Master Theses, Seoul National University, Korea. Kurata, N., Y. Nagamura, K. Yamamoto, Y. Harushima, N. Sue, J. Wu, B.A. Antonio, A. Shomura, T. Shimizu, S.Y. Lin, and et al. 1994. A 300 kilobase interval genetic map of rice including 883 expressed sequences. Nat Genet 8: 365-72. Laffont, J. L., K. M. Remund, D. Wright, R. D. Simpson and S. Gregoire. 2005. Testing for adventitious presence of transgenic material in conventional seed or grain lots using quantitative laboratory methods: statistical procedures and their implementation. Seed Science Research 15 (3): 197-204. Liu, R.H., and J.L. Meng. 2003. [MapDraw: a microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data]. Yi Chuan 25: 317-21. Little,S. (1997) ARMS analysis of point mutatons. In Taylor,G.R. (ed.) Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA. CRC Press, Boca Raton, FL, pp. 45–51. Monna, L., R. Ohta, H. Masuda, A. Koike, and Y. Minobe. 2006. Genome-wide searching of single-nucleotide polymorphisms among eight distantly and closely related rice cultivars (Oryza sativa L.) and a wild accession (Oryza rufipogon Griff.). DNA Res 13: 43-51. Nasu, S., J. Suzuki, R. Ohta, K. Hasegawa, R. Yui, N. Kitazawa, L. Monna, and Y. Minobe. 2002. Search for and analysis of single nucleotide polymorphisms (SNPs) in rice (Oryza sativa, Oryza rufipogon) and establishment of SNP markers. DNA Res 9: 163-71. Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey, and A.R. . 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. . Mol Breed 2: 225-238. Remund, K. M., D. A. Dixon, D. L. Wright and L. R. Holden. 2001. Statistical considerations in seed purity testing for transgenic traits. Seed Science Research 11 (2): 101-119. Rice genome research program. 2007. http://rgp.dna.affrc.go.jp/E/index.html Shen, Y.J., H. Jiang, J.P. Jin, Z.B. Zhang, B. Xi, Y.Y. He, G. Wang, C. Wang, L. Qian, X. Li, Q.B. Yu, H.J. Liu, D.H. Chen, J.H. Gao, H. Huang, T.L. Shi, and Z.N. Yang. 2004. Development of genome-wide DNA polymorphism database for map-based cloning of rice genes. Plant Physiol 135: 1198-205. Singh, V.K., A.K. Mangalam, S. Dwivedi, and S. Naik. 1998. Primer premier: Program for design of degenerate primers from a protein sequence. Biotechniques 24: 318-319. Smith, J.S.C., E. C. L. Chin, H. Shu, O. S. Smith, S. J. Wall, M. L. Senior, S. E. Michell, S. Kresovick, and J. Ziegle. 1997. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L):Comparison with data from RFLPs and pedigrees. . Theor Appl Genet 95: 163-173. Wittwer, C.T., G.H. Reed, C.N. Gundry, J.G. Vandersteen, and R.J. Pryor. 2003. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem 49: 853-60. Wu, J.Y., H.K. Wu, and M.C. Chung. 2002. Co-dominant RAPD markers closely linked with two morphological genes in rice (Oryza sativa L.). Botanical Bulletin of Academia Sinica 43: 171-180. Yamamoto, K., and T. Sasaki. 1997. Large-scale EST sequencing in rice. Plant Mol Biol 35: 135-44. Ye, S., S. Dhillon, X. Ke, A.R. Collins, and I.N. Day. 2001. An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res 29: E88-8. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27886 | - |
dc.description.abstract | 為鑑別台灣地區主要良質米稉稻品種,本試驗一共篩選了10 組SNP (Single Nucleotide Polymorphisms) 標記,152組InDel (insertion / deletion) 標記,156 組STS (Sequence tagged sites) 標記。最後在21個稉稻品種中得到具多型性的4組SNP標記、30組InDel標記和25 組STS標記,共59組可在一般電泳膠片中高通量分析之多型性標記;排除顯隱性多型性、需較精細調控的四引子系統及多型性重複的標記後,共有34組共顯性多型性標記。利用此34組獨特多型性可選出五組各含7組標記的最小鑑別組合,可完全鑑別21個稉稻品種。根據所得的共顯性標記應用於混合樣品分析,對於混雜率的檢測能力可以達到10%,對於品種檢查時樣品數較大的分析需求也能具有快速分析的優點。 | zh_TW |
dc.description.abstract | In order to identify the major japonica rice cultivars in Taiwan, 10 single nucleotide polymorphisms (SNP) markers, 152 insertion/deletion (InDel) markers and 156 sequence tagged sites (STS) markers were screened against 21 japonica rice cultivars. Among the 318 markers screened, 59 of them are polymorphic, including 4 SNP markers, 30 InDel markers, and 25 STS markers. After eliminating dominant markers, tetra primer markers and replications of polymorphisms, there are 34 co-dominant markers showing unique polymorphic patterns. Five minimum sets of markers that can identify each of the 21 japonica rice cultivars completely from these 34 unique polymorphic markers contains only 7 markers. The sensitivity for testing bulk samples reach 10% while using these co-dominant markers, and therefore suitable for analyzing large amount of samples during variety testing. | en |
dc.description.provenance | Made available in DSpace on 2021-06-12T18:26:00Z (GMT). No. of bitstreams: 1 ntu-96-R94621120-1.pdf: 10619029 bytes, checksum: a7ebd1944bb49c065315179fada55afd (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 內容目錄 i
圖片目錄 iii 表格目錄 v 摘要 vi ABSTRACT vii 壹、 前言 1 貳、 前人研究 2 參、 材料與方法 5 一、 材料 5 二、 水稻葉片DNA抽取 7 三、 稉稻品種間單一核苷酸多型性的篩選 7 四、 稉稻品種間插入、缺失 (InDel) 多型性之引子篩選 10 五、 稉稻品種間STS之引子篩選 12 六、 混合樣品分析 13 七、 資料分析 14 肆、 結果與討論 15 一、 篩選所得之多型性標記 15 SNP 15 InDel 22 STS 24 稉稻間多型性分子標記 24 二、 最小鑑別組合 30 三、 參試之稉稻、秈稻相似性比較 31 四、 檢測混合樣品 33 五、 比較SNP與InDel標記 35 伍、 結論 38 陸、 參考文獻 39 柒、 附錄 42 附錄一、所篩選之STS標記 42 附錄二、34組多型性標記電泳結果 46 | |
dc.language.iso | zh-TW | |
dc.title | 鑑別台灣主要稉稻品種的InDel標記之開發 | zh_TW |
dc.title | Development of InDel Markers for Variety Identification amomg Major Japonica Rice Cultivars in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蘇宗振,王裕文 | |
dc.subject.keyword | 稉,稻,分子標記,品種鑑別, | zh_TW |
dc.subject.keyword | japonica rice,marker,variety Identification, | en |
dc.relation.page | 48 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-08-13 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
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