新型多孔性幾丁聚醣微粒製備法

Abstract

本研究建立一種新型多孔性幾丁聚醣微粒之製程，並將製成之微粒用於負載牛血清白蛋白(bovine serum albumin，簡稱BSA)與槲皮素(quercetin)，最後將其負載能力與先前研究之結果相互比較。 本研究所製成之微粒依製程參數不同，其平均粒徑大小約為350 ~ 70 μm，且粒徑分佈在粒徑大時較為集中(變異係數(C.V.)值約為26.45 %)，而在粒徑小時則較為寬廣(C.V.為53.89 %)。關於微粒吸附特性之探討，在相同BSA初始吸附濃度(3 mg/mL)時，本法所製成微粒之吸附量約為400 ~ 500 mg / g chitosan，約為本研究室先前研究所製成微粒吸附量之2 ~ 3倍。在槲皮素初始吸附濃度為10 μg/mL時，微粒之吸附量約為先前研究在相同初始吸附濃度下的10倍，而達平衡吸附量之90 % 時的平均吸附速率亦為先前研究之3 ~ 4倍。 本研究開發了在無添加任何界面活性劑之情況下，製備出球型多孔性幾丁聚醣微粒之技術。在微粒之吸附能力上，因其多孔結構之特性，亦有相當程度之提升。

In this research, a novel method for the preparation of porous chitosan microparticles was developed and the produced microparticles were used in the BSA and quercetin isothermal adsorption, and the results were compared with previous data. In this study, the mean sizes of the microparticles were around 350 to 70 μm under different operation conditions, and the size distribution was concentrated (coefficient of variance (C.V.) = 26.45 %) for particles with large mean diameters, broad (C.V. = 53.89 %) for small particles. At the same initial concentration (3 mg/mL) of BSA, the amount of BSA adsorbed on the microparticles produced in this study was about 400 ~ 500 mg BSA/g chitosan, which was 2 to 3 times the data of the previous research in our lab. The amount of the quercetin adsorbed on the microparticles was about 10 times the data of the previous research and the average adsorption rate was also enhanced 3 to 4 times. In this study, the spherical porous chitosan microparticles were successfully prepared without adding any surfactant. Also, the adsorption capability ability of the microparticles was improved due to the porous structure.